Abstract:
:The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, RealSeq®-AC provides greatly reduced miRNA sequencing bias and allows the identification of the largest variety of miRNAs in biological samples. This reduced bias also allows robust quantification of miRNAs present in samples across a wide range of RNA input levels.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Barberán-Soler S,Vo JM,Hogans RE,Dallas A,Johnston BH,Kazakov SAdoi
10.1186/s13059-018-1488-zsubject
Has Abstractpub_date
2018-09-03 00:00:00pages
105issue
1eissn
1474-7596issn
1474-760Xpii
10.1186/s13059-018-1488-zjournal_volume
19pub_type
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