当前位置： SCI文献检索 > Molecular Medicine Reports期刊下所有文献 > Role of autophagy in a model of obesity: A long‑term high fat diet induces cardiac dysfunction.

Role of autophagy in a model of obesity: A long‑term high fat diet induces cardiac dysfunction.

Abstract：

:Obesity may induce end‑organ damage through metabolic syndrome, and autophagy serves a vital role in the pathogenesis of metabolic syndrome. The purpose of the present study was to define the roles of autophagy and mitophagy in high fat diet (HFD)‑induced cardiomyopathy. Male, 8 week‑old C57BL/6 mice were fed either a HFD (60% kcal) or a diet of normal chow (NC; 10% kcal) for 42 weeks. Glucose tolerance tests were performed during the feeding regimes. Blood samples were collected for assaying serum triglyceride with the glycerol‑3‑phosphate oxidase phenol and aminophenazone (PAP) method and total cholesterol was tested with the cholesterol oxidase‑PAP method. Myocardial function was assessed using echocardiography and hemodynamic analyses. Western blot analysis was employed to evaluate endoplasmic reticulum stress (ERS), autophagy and mitochondrial function. Electron microscopy was used to assess the number of lipid droplets and the degree of autophagy within the myocardium. The body weight and adipose tissue weight of mice fed the HFD were increased compared with the NC mice. The serum levels of blood glucose, total cholesterol and triglyceride were significantly increased following 42 weeks of HFD feeding. The results of the glucose tolerance tests additionally demonstrated metabolic dysregulation in HFD mice. In addition, HFD mice exhibited hemodynamic and echocardiographic evidence of impaired diastolic and systolic function, including alterations in the cardiac output, end‑diastolic pressure, end‑diastolic volume and left ventricular relaxation time constant (tau) following HFD intake. Furthermore, a HFD resulted in increased ERS, and a downregulation of the autophagy and mitophagy level. The present study investigated cardiac function in obese HFD‑fed mice. These results aid the pursuit of novel therapeutic targets to combat obesity‑associated cardiomyopathy.

journal_name

Mol Med Rep

journal_title

Molecular medicine reports

authors

Che Y,Wang ZP,Yuan Y,Zhang N,Jin YG,Wan CX,Tang QZ

doi

10.3892/mmr.2018.9301

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

3251-3261

issue

eissn

1791-2997

issn

1791-3004

journal_volume

pub_type

杂志文章

在线工具

Role of autophagy in a model of obesity: A long‑term high fat diet induces cardiac dysfunction.