Abstract：
:Sexual assault evidence samples require the use of a specific process known as a differential digestion to separate sperm from nonsperm cells prior to DNA extraction. An automated differential digestion process was developed using a selective degradation technique, which uses DNase I to digest the remaining nonsperm DNA in the sperm fraction. The use of DNase on pristine samples, as well as aged and degraded samples, was assessed to ensure that the quantity and quality of the sperm DNA were not compromised or adversely affected. Samples processed using the selective degradation technique yielded comparable DNA yield and DNA typing data to the conventional differential digestion process. The automated process utilized 96-well plates for high throughput and incorporated microscope slide preparations for confirmation of sperm. It reduced processing time by about sixfold and was paramount in the elimination of the Oakland Police Department Criminalistics Laboratory's sexual assault kit backlog.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Wong H,Mihalovich Jdoi
10.1111/1556-4029.13877subject
Has Abstractpub_date
2019-03-01 00:00:00pages
539-550issue
2eissn
0022-1198issn
1556-4029journal_volume
64pub_type
杂志文章abstract：:A simple, rapid radioimmunoassay employing 125-I-labeled secobarbital derivative has been developed and has been shown to be capable of detecting at the nanogram level a variety of barbiturates in urine as well as in plasma. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Adermatoglyphia is a very rare autosomal-dominant condition that is genetically inherited and causes an individual to be born without conventional ridge detail on either their palmar or plantar surfaces (the fingers and palms of the hands and the toes and the soles of the feet). While adermatoglyphia has been the focu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14597
更新日期：2021-01-01 00:00:00
abstract：:Four cases of homicide, in which silenced firearms were used, are reported and supplemented by data from experimental investigations regarding wound features, marks, traces, and ballistic behavior. Wound features are largely determined by the construction of the silencer. In one case, even a muzzle imprint was produce...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:Genipin, a hydrolytic product of geniposide extracted from gardenia fruit, was thoroughly studied as a potential fingerprint reagent, and optimal conditions for fingerprint development have been determined. Latent fingerprints on paper items that have been treated with a non-ink running formulation containing 0.17% of...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Passive infrared technology was used to film diurnal and nocturnal scavenging behavior of brown rats and gray squirrels at the University of Tennessee's Anthropological Research Facility. This direct documentation demonstrated that brown rats modified fat-laden cancellous bone while gray squirrels generally gnawed the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00467.x
更新日期：2007-07-01 00:00:00
abstract：:Alcohol-related amnesia--alcohol blackout--is a common claim of criminal defendants. The generally held belief is that during an alcohol blackout, other cognitive functioning is severely impaired or absent. The presentation of alcohol blackout as scientific evidence in court requires that the science meets legal relia...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：10.1111/1556-4029.12134
更新日期：2013-07-01 00:00:00
abstract：:Cardiovascular disease is the leading cause of sudden death in the world. The etiology of sudden cardiac death involves a wide range of diseases, but seldom pericardial cysts. A pericardial cyst is an uncommon cyst usually located in the middle mediastinum and rarely in the posterior part. They are usually harmless an...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.13826
更新日期：2019-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12795
更新日期：2015-09-01 00:00:00
abstract：:In late June 1990, the Mono County Sheriffs Department in Bridgeport, CA contacted the Physical Anthropology Human Identification Laboratory (PAHIL) at California State University, Chico to seek assistance in the identification of a recently discovered skull. To assist with possible identification, the cranium receive...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:The estimation of postmortem interval (PMI) based on successional patterns of adult insects is largely limited, due to the lack of potential PMI markers. Sex and size of adult insects could be easily used for such estimation. In this study, sex- and size-related patterns of carrion attendance by adult insects were ana...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13376
更新日期：2017-09-01 00:00:00
abstract：:A sample of 31 criminal defendants who were charged with murder but without major mental disorder was studied. The sample was derived from a large urban multi-ethnic, multi-cultural community pool. Subject characteristics and information about the homicide and decedents are described. Suggestions for further study are...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:The authors present the medico-legal investigations and identification after the aircrash of the Airbus A320 upon the Mount Sainte-Odile (France). The identification team comprising investigators from the gendarmerie, forensic pathologists, odontologists, and scientists of the Institute from Legal Medecine rapidly ret...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:In cases of acute fatal child abuse, certain injuries, including cutaneous blunt force trauma, skull fractures, subdural hematomas, intra-abdominal hemorrhage, and retinal hemorrhages are common and well described in the pediatric and forensic literature. These gross findings at autopsy, when taken into consideration ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00883.x
更新日期：2008-11-01 00:00:00
abstract：:The allele frequency distributions of three STR loci amplified by PCR have been studied in four Israeli communities: Ashkenazi Jews and three non-Ashkenazi groups, namely Moroccan, Yemenite, and Ethiopian Jews. The loci analyzed were CSF1PO, TPOX, and HUMTHO1. The typing was performed in sequencing polyacrylamide gels...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:From the works of Broca and Krogman to modern-day Jantz and Buikstra, the orbit has been used for both quantitative and qualitative sex and race estimation. This study evaluates the practical value of these estimations. Orbital height and breadth were measured to determine the orbital index and assess differences betw...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01864.x
更新日期：2011-11-01 00:00:00
abstract：:A state law has placed an obligation on the criminal investigation laboratories to screen and quantitate all preparations, both pharmaceutical and illicit, that fall under the provisions of "Excepted Substances." Without complete screens on these preparations, there is room for erroneous indictments as well as possibl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:A sensitive, specific and simple color test for the improvised explosive urea nitrate is described. It is based on the formation of a red pigment upon the reaction between urea nitrate and p-dimethylaminocinnamaldehyde (p-DMAC) under neutral conditions. Urea itself, which is the starting material for urea nitrate, doe...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:Ignitable liquid residues recovered from a fire scene will often show signs of weathering as a result of exposure to the heat of the fire. In addition, when the substrate is rich in organic matter, both weathering and microbial degradation may be observed. In this study, 20 μL aliquots of fresh gasoline samples were i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01989.x
更新日期：2012-01-01 00:00:00
abstract：:Knowledge concerning the distribution of methadone in postmortem human tissue and the effect of postmortem redistribution on methadone is today limited making the choice of a suitable substitute for femoral blood difficult when this is not available. Cardiac blood, femoral blood, muscle, and brain tissue concentration...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12627
更新日期：2015-01-01 00:00:00
abstract：:Progress in the understanding of individuals who commit arson has been on a slow but steady course over the past two decades. From our review of court-ordered outpatient forensic psychiatric evaluation of individuals charged with arson over a five-year period, preliminary prototypical profiles of the psychotic, mental...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:We have previously reported a new triplex amplification and typing system by silver staining for three short tandem repeat (STR) loci, 9q2h2 (D2S3020), D15S233, and D14S299 without "microvariant" alleles such as .1, .2, and, .3 alleles in the Japanese population. In the present study, we established a new quadruplex s...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:The ratio of blood-alcohol concentration (BAC) to breath-alcohol concentration (BrAC) was determined for 799 individuals apprehended for driving under the influence of alcohol (DUI) in Sweden. The BrAC was determined with an infrared analyzer (Intoxilyzer 5000S) and venous BAC was measured by headspace gas chromatogra...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:This study examined the effects of heat on the amplification of DNA from the dental pulp of Sus scrofa molars and investigated the protection afforded to the pulp tissue by the dental enamel, alveolar process, and soft tissue of the head. Segments of defleshed maxilla and mandible encasing the first molar (n = 60) wer...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01286.x
更新日期：2010-03-01 00:00:00
abstract：:The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01818.x
更新日期：2011-09-01 00:00:00
abstract：:The increased marketing of fake perfumes has encouraged us to investigate how to identify such products by their chemical characteristics and multivariate analysis. The aim of this study was to present an alternative approach to distinguish original from fake perfumes by means of the investigation of sodium, potassium...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13070
更新日期：2016-07-01 00:00:00
abstract：:The present study reexamines the accuracy of the supero-inferior femoral neck diameter for the determination of sex using a modern sample of French individuals. In 1998, Seidemann et al. used this univariate method for sex determination with the Hamann-Todd collection. Stojanowski and Seidemann in 1999 tested previous...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:In recent years, there has been a renewed interest in forensic sciences about forensic age estimation in living subjects by means of radiological methods. This research was conducted on digital thorax X-rays to test the usefulness of some radiological changes in the clavicle and first rib. The sample consisted in a to...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01626.x
更新日期：2011-01-01 00:00:00
abstract：:The analysis of bloodstain patterns can assist investigators in understanding the circumstances surrounding a violent crime. Bloodstains are routinely subjected to pattern analysis, which is inherently dependent upon the ability of the examiner to locate and visualize bloodstain patterns on items of evidence. Often, t...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02171.x
更新日期：2012-11-01 00:00:00
abstract：:Internal standards are commonly used for the quantitative determination of drugs of abuse and their metabolites (drug/metabolite) in biological fluids and tissues by the selective ion monitoring (SIM) gas chromatography/mass spectrometry (GC/MS) procedure. Analogs of drugs/metabolites that are labeled with three or mo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Soil vestiges might provide information about a crime scene. The Rietveld method with X-ray diffraction data (RM-XRD) is a nondestructive technique that makes it possible to characterize minerals present in the soils. Soil clays from the metropolitan region of Curitiba (Brazil) were submitted to DCB treatment and anal...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13476
更新日期：2018-01-01 00:00:00
