Abstract:
:Synthetic biology aims at (re-)programming living cells like computers to perform new functions for a variety of applications. Initial work rested on transcription factors, but regulatory RNAs have recently gained much attention due to their high programmability. However, functional circuits mainly implemented with regulatory RNAs are quite limited. Here, we report the engineering of a fundamental arithmetic logic unit based on de novo riboregulation to sum two bits of information encoded in molecular concentrations. Our designer circuit robustly performs the intended computation in a living cell encoding the result as fluorescence amplitudes. The whole system exploits post-transcriptional control to switch on tightly silenced genes with small RNAs, together with allosteric transcription factors to sense the molecular signals. This important result demonstrates that regulatory RNAs can be key players in synthetic biology, and it paves the way for engineering more complex RNA-based biocomputers using this designer circuit as a building block.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Rosado A,Cordero T,Rodrigo Gdoi
10.1371/journal.pgen.1007548subject
Has Abstractpub_date
2018-07-19 00:00:00pages
e1007548issue
7eissn
1553-7390issn
1553-7404pii
PGENETICS-D-18-00497journal_volume
14pub_type
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