Effect of Autophagy Inhibition on the Protection of Ischemia Preconditioning against Myocardial Ischemia/Reperfusion Injury in Diabetic Rats.

Abstract:

Background:Ischemia preconditioning (IPC) remains the most powerful intervention of protection against myocardial ischemia/reperfusion injury (IRI), but diabetes can weaken or eliminate its cardioprotective effect and detailed mechanisms remain unclear. In this study, we aimed to explore whether changes of autophagy in the diabetic condition are attributable to the decreased cardioprotective effect of IPC. Methods:Sixty diabetic male Sprague-Dawley rats were randomly divided into the control (C), IRI, rapamycin (R), wortmannin (W), rapamycin + IPC (R + IPC), and wortmannin + IPC (W + IPC) groups. The in vivo rat model of myocardial IRI was established by ligaturing and opening the left anterior descending coronary artery via the left thoracotomy. Durations of ischemia and reperfusion are 30 min and 120 min, respectively. Blood samples were taken at 120 min of reperfusion for measuring serum concentrations of troponin I (TnI) and creatine kinase isoenzyme MB (CK-MB) using the enzyme-linked immunosorbent assay. The infarct size was assessed by Evans blue and triphenyltetrazolium chloride staining. The expressions of LC3-II, beclin-1, phosphoinositide 3-kinase (PI3K), mammalian target of rapamycin (mTOR), and P-Akt/Akt ratio in the ischemic myocardium were assessed by Western blotting. Results:Compared to the IRI group, infarct size (56.1% ± 6.1% vs. 75.4 ± 7.1%, P < 0.05), serum cTnI (0.61 ± 0.21 vs. 0.95 ± 0.26 ng/ml, P < 0.05), and CK-MB levels (6.70 ± 1.25 vs. 11.51 ± 2.35 ng/ml, P < 0.05) obviously decreased in the W + IPC group. Compared with the C group, myocardial expressions of LC3-II (0.46 ± 0.04 and 0.56 ± 0.04 vs. 0.36 ± 0.04, P < 0.05) and beclin-1 (0.34 ± 0.08 and 0.38 ± 0.07 vs. 0.24 ± 0.03, P < 0.05) evidently increased, and myocardial expressions of mTOR (0.26 ± 0.08 and 0.25 ± 0.07 vs. 0.38 ± 0.06, P < 0.05), PI3K (0.29 ± 0.04 and 0.30 ± 0.03 vs. 0.38 ± 0.02, P < 0.05), and P-Akt/Akt ratio (0.49 ± 0.10 and 0.48 ± 0.06 vs. 0.72 ± 0.07, P < 0.05) markedly decreased in the IRI and R groups, indicating an increased autophagy. Compared with the IRI group, myocardial expression of beclin-1 (0.26 ± 0.03 vs. 0.34 ± 0.08, P < 0.05) significantly decreased, and myocardial expressions of mTOR (0.36 ± 0.04 vs. 0.26 ± 0.08, P < 0.05), PI3K (0.37 ± 0.03 vs. 0.29 ± 0.04, P < 0.05), and P-Akt/Akt ratio (0.68 ± 0.05 vs. 0.49 ± 0.10, P < 0.05) increased obviously in the W + IPC group, indicating a decreased autophagy. Conclusions:Increased autophagy in the diabetic myocardium is attributable to decreased cardioprotection of IPC, and autophagy inhibited by activating the PI3K-Akt-mTOR signaling pathway can result in an improved protection of IPC against diabetic myocardial IRI. :自噬抑制对缺血预处理保护糖尿病大鼠心肌缺血/再灌注损伤的影响摘要背景:缺血预处理(Ischemic preconditioning, IPC)仍然是心肌缺血/再灌注损伤(Ischemia/reperfusion injury,IRI)最强效的保护干预措施,但糖尿病可减弱甚至消除其心肌保护作用,并且具体机制尚不明确。本研究旨在探讨糖尿病条件下自噬改变是否参与了降低的IPC心肌保护作用。 方法:将60只雄性糖尿病Sprague-Dawley大鼠随机分为对照组(C组)、IRI组、雷帕霉素组(R组)、渥曼青霉素组(W组)、雷帕霉素+IPC组(R+IPC组)和渥曼青霉素+IPC组(W+IPC组)。通过左侧开胸结扎和开放左冠状动脉前降支建立大鼠在体心肌IRI模型,心肌缺血和再灌注的时间分别是30分钟和120分钟。再灌注120 min时采集血样,采用酶联免疫吸附法(enzyme-linked immunosorbent essay, ELISA)检测血清心肌肌钙蛋白I(cardiac troponin I,cTnI)和肌酸激酶同工酶MB(creatine kinase isoenzyme MB,CK-MB);采用伊文氏蓝和2,3,5-三苯基氯化四氮唑双染色法测定心肌梗死面积;采用Western-blotting检测LC3-Ⅱ、Beclin-1、mTOR、PI3K心肌表达情况和P-Akt/Akt比率。 结果:与IRI组相比,W+IPC组的心肌梗死面积(56.1%±6.1%比75.4±7.1%,P <0.05),血清cTnI浓度(0.61±0.21比0.95±0.26 ng/ml,P <0.05)、血清CK-MB浓度(6.70±1.25比11.51±2.35 ng/ml,P <0.05)均明显降低;与C组相比,IRI和R组LC3-Ⅱ(0.46±0.04和0.56±0.04比0.36±0.04,P <0.05)和beclin-1(0.34±0.08和0.38±0.07比0.24±0.03,P <0.05)心肌表达明显增强,mTOR(0.26±0.08和0.25 ± 0.07 比 0.38 ± 0.06, P < 0.05)和PI3K(0.29±0.04和0.30±0.03比0.38±0.02,P <0.05)心肌表达以及P-Akt/Akt比率(0.49±0.10和0.48 ± 0.06比0.72 ± 0.07, P < 0.05)明显降低,表明自噬表达增强。与IRI组相比,W+IPC组beclin-1(0.26±0.03比0.34±0.08,P <0.05)心肌表达明显降低,mTOR(0.36±0.04比0.26±0.08,P <0.05)和PI3K(0.37±0.03比0.29±0.04,P <0.05)心肌表达以及P-Akt/Akt比率(0.68±0.05比0.49±0.10,P <0.05)明显增加,表明自噬表达降低。 结论:糖尿病心肌自噬表达增强可减弱IPC的心肌保护作用,激活PI3K-Akt-mTOR信号转导通路抑制自噬可改善IPC对糖尿病心肌IRI的保护作用。.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Liu YY,Sun C,Xue FS,Yang GZ,Li HX,Liu Q,Liao X

doi

10.4103/0366-6999.235867

subject

Has Abstract

pub_date

2018-07-20 00:00:00

pages

1702-1709

issue

14

eissn

0366-6999

issn

2542-5641

pii

ChinMedJ_2018_131_14_1702_235867

journal_volume

131

pub_type

杂志文章
  • Uncommon characteristics of the structure and development of Trichosporon asahii.

    abstract:BACKGROUND:Trichosporon asahii (T. asahii) is one of the most important pathogenic fungus in the genus of trichosporon. Although the species identification of T. asahii was based upon the complicated results of morphologic, biochemical and biologic examination, the morphology characteristic is still the first clue to t...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang WL,Yang RY,Ao JH

    更新日期:2009-08-05 00:00:00

  • Extended parietal cell vagotomy in the treatment of perforation, hemorrhage and stenosis due to duodenal ulcer.

    abstract::Ninety-five patients with perforation, hemorrhage or stenosis due to duodenal ulcer were treated by extended parietal cell vagotomy. Postoperative follow-up ranged from 3.5 to 10 years (mean 6 years) in 88 patients (92%) with acute perforation (60), hemorrhage (8) and stenosis (20). There was no operative mortality. U...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li SY,An P,Liang ZJ,Yuan SJ,Yu J

    更新日期:1992-04-01 00:00:00

  • Role of plasma C-reactive protein in predicting in-stent restenosis in patients with stable angina after coronary stenting.

    abstract:BACKGROUND:The role of plasma high sensitivity C-reactive protein (hs-CRP) in in-stent restenosis (ISR) remains controversial. We investigated plasma hs-CRP level at both admission and follow-up in patients with stable angina (SA) after successful coronary stenting in order to clarify the predictive value of hs-CRP for...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Xu YL,Li JJ,Xu B,Zhu CG,Yang YJ,Chen JL,Qiao SB,Yuan JQ,Qin XW,Ma WH,Yao M,Liu HB,Wu YJ,Chen J,You SJ,Dai J,Xia R,Gao RL

    更新日期:2011-03-01 00:00:00

  • Supplementary inferior phrenic artery embolization in the interventional treatment of hemoptysis.

    abstract:BACKGROUND:Transcatheter bronchial artery embolization (BAE) is widely used for the treatment of hemoptysis and the immediate success rate is high, but there are still some hemorrhage recurrences. One of the common reasons for failure of BAE is collateral branches as blood supply. The inferior phrenic artery (IPA) is o...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Liu FY,Wang MQ,Fan QS,Duan F,Wang ZJ,Song P

    更新日期:2009-03-05 00:00:00

  • Preparation of collagen-based materials for wound dressing.

    abstract:OBJECTIVE:To describe the methods which were used to develop collagen-based materials for wound dressing. METHODS:Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wu Z,Sheng Z,Sun T,Geng M,Li J,Yao Y,Huang Z

    更新日期:2003-03-01 00:00:00

  • Value of Combining Left Atrial Diameter and Amino-terminal Pro-brain Natriuretic Peptide to the CHA2DS2-VASc Score for Predicting Stroke and Death in Patients with Sick Sinus Syndrome after Pacemaker Implantation.

    abstract:BACKGROUND:The CHA2DS2-VASc score is used clinically for stroke risk stratification in patients with atrial fibrillation (AF). We sought to investigate whether the CHA2DS2-VASc score predicts stroke and death in Chinese patients with sick sinus syndrome (SSS) after pacemaker implantation and to evaluate whether the pre...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.211881

    authors: Mo BF,Lu QF,Lu SB,Xie YQ,Feng XF,Li YG

    更新日期:2017-08-20 00:00:00

  • Autophagy in 5-Fluorouracil Therapy in Gastrointestinal Cancer: Trends and Challenges.

    abstract:OBJECTIVE:5-Fluorouracil (5-FU)-based combination therapies are standard treatments for gastrointestinal cancer, where the modulation of autophagy is becoming increasingly important in offering effective treatment for patients in clinical practice. This review focuses on the role of autophagy in 5-FU-induced tumor supp...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:10.4103/0366-6999.176069

    authors: Tang JC,Feng YL,Liang X,Cai XJ

    更新日期:2016-02-20 00:00:00

  • The clinical and epidemiological significance of serum PreS1/anti-PreS1 in HBV infection.

    abstract:OBJECTIVE:To investigate whether PreS1 antigen and/or anti-PreS1 antibody in serum might have some significance different from routine hepatitis B virus (HBV) markers in hepatitis B virus infection. METHODS:Forty-six acute hepatitis B patients and 377 chronic persistent hepatitis patients were examined to assess the c...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Mi Z,Feng F,Zhang X,Lian Z,Wang H,Tong Y

    更新日期:1999-04-01 00:00:00

  • Preliminary results of combined carotid endarterectomy and off-pump coronary artery bypass grafting in patients with coexistent carotid and coronary artery diseases.

    abstract:BACKGROUND:Coexistent carotid and coronary artery diseases are common and patients with them remain at a high risk for perioperative stroke or myocardial infarction after coronary bypass surgery. The aim of this study was to investigate the effect of combined carotid endarterectomy (CEA) and off-pump coronary artery by...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chen XJ,Chen X,Xie DH,Shi KH,Xu M

    更新日期:2009-12-20 00:00:00

  • Effect of RNA Interference Targeting STAT3 Gene Combined with Ultrasonic Irradiation and SonoVue Microbubbles on Proliferation and Apoptosis in Keratinocytes of Psoriatic Lesions.

    abstract:Background:Signal transducer and activator of transcription 3 (STAT3) was strongly expressed and activated in psoriatic keratinocytes (KCs) and correlated with the severity of psoriasis. The study aimed to investigate the effects of STAT3 small interfering RNA (siRNA) combined with ultrasonic irradiation and SonoVue mi...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.239297

    authors: Ran LW,Wang H,Lan D,Jia HX,Yu SS

    更新日期:2018-09-05 00:00:00

  • Cement vertebroplasty combined with ethanol injection in the treatment of vertebral hemangioma.

    abstract:BACKGROUND:A number of methods have been used in the treatment of symptomatic and aggressive vertebral hemangioma, but none of them is optimal. Vertebral hemangioma treated with cement vertebroplasty or ethanol injection alone showed relatively good results despite their limitations. METHODS:Between February 2002 and ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chen L,Zhang CL,Tang TS

    更新日期:2007-07-05 00:00:00

  • Beneficial effects of a diabetes specific formula on insulin sensitivity and free fatty acid in patients with type 2 diabetes mellitus.

    abstract:BACKGROUND:This prospective, randomized, controlled study was designed to investigate the effects of a diabetes specific formula (Diason low energy: 313.8 kJ/100 ml), compared with a standard formula, on insulin sensitivity, serum C peptide, serum lipids and free fatty acid (FFA) in type 2 diabetics. METHODS:In total ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: Li YX,Zeng JB,Yu K,Sun Q,Liu QY,Qin W,Zhang Q,Yu JC,Wang H

    更新日期:2008-04-20 00:00:00

  • Effect of glutamine enriched nutrition support on surgical patients with gastrointestinal tumor: a meta-analysis of randomized controlled trials.

    abstract:BACKGROUND:Associations between glutamine (Gln) enriched nutrition support and surgical patients with gastrointestinal (GI) tumor remain controversy. The purpose of this meta-analysis was to assess the effect of Gln enriched nutrition support on surgical patients with GI tumor in term of relevant biochemical indices, i...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,meta分析

    doi:10.4103/0366-6999.149219

    authors: Kang K,Shu XL,Zhang YS,Liu XL,Zhao J

    更新日期:2015-01-20 00:00:00

  • Murine calcium-activated chloride channel family member 3 induces asthmatic airway inflammation independently of allergen exposure.

    abstract:BACKGROUND:Expression of murine calcium-activated chloride channel family member 3 (mCLCA3) has been reported to be increased in the airway epithelium of asthmatic mice challenged with ovalbumin (OVA). However, its role in asthmatic airway inflammation under no OVA exposure has not yet been clarified. METHODS:mCLCA3 p...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Mei L,He L,Wu SS,Zhang B,Xu YJ,Zhang ZX,Zhao JP,Zhang HL

    更新日期:2013-01-01 00:00:00

  • Radiographic measurement of the prevertebral soft tissue of cervical vertebrae.

    abstract::The radiographic measurement of the prevertebral soft tissue of cervical vertebrae was performed in 87 normal adults. According to the results of the measurement, 10 mm and 20 mm were used as the upper limit normal values of the retropharyngeal and retrotracheal space respectively. We conclude that although the widene...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Dai LY,Jia LS

    更新日期:1994-06-01 00:00:00

  • Endovascular treatment of cerebral arteriovenous malformations with Onyx embolization.

    abstract:BACKGROUND:In the past 5 years, new treatment materials and techniques offering a different concept in endovascular treatment have been described for cerebral arteriovenous malformations (CAVMs). The aim of this study was to assess the endovascular treatment of CAVMs by using a liquid embolic material, Onyx (Micro Ther...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: He HW,Jiang CH,Liu HB,Li YX,Zhang JB,Wu ZX

    更新日期:2005-12-20 00:00:00

  • Clinical and pathological studies of borderline gastrointestinal stromal tumors.

    abstract:BACKGROUND:Borderline gastrointestinal stromal tumors (GISTs) are intermediate tumors between benign and malignant variants; however, the clinical and pathological features of borderline GISTs remain poorly defined. This study aimed to characterize GISTs and to identify a set of borderline criteria for practical use. ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Shi Y,Hou YY,Lu SH,Zhou Y,Xu JF,Ji Y,Hou J,Xu C,Liu YL,Tan YS,Zhu XZ

    更新日期:2010-09-01 00:00:00

  • High-frequency Oscillations and the Seizure Onset Zones in Neocortical Epilepsy.

    abstract:BACKGROUND:To study the characters of high-frequency oscillations (HFOs) in the seizure onset zones (SOZ) and the nonseizure onset zones (NSOZ) in the electrocorticography (ECoG) of patients with neocortical epilepsy. METHODS:Only patients with neocortical epilepsy who were seizure-free after surgery as determined wit...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.159342

    authors: Sun YP,Wang YP,Wang ZH,Wu FY,Tang LO,Zhang SW,Pei HT,Wang Y,Huang ZY,Xue Q,Xu CP,Tai JL

    更新日期:2015-07-05 00:00:00

  • Value of polymerase chain reaction assay in diagnosis of intestinal tuberculosis and differentiation from Crohn's disease.

    abstract::It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intestinal tuberculosis from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were su...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Gan H,Ouyang Q,Bu H,Li S,Chen D,Li G,Yang X

    更新日期:1995-03-01 00:00:00

  • Passive graft perfusion in off-pump coronary artery bypass grafting.

    abstract:BACKGROUND:Myocardial protection during off-pump coronary artery bypass grafting (OPCABG) is a multifactorial problem in which maintaining stable systemic hemodynamics is very important. In this study passive graft perfusion (PGP) was applied to investigate the effect during and after OPCABG as evaluated by cardiac tro...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: Lu F,Ji BY,Liu JP,Liu MZ,Wang GY,Hu SS

    更新日期:2007-02-05 00:00:00

  • Spinal release of the amino acids with a time course in a rat model of postoperative pain.

    abstract:BACKGROUND:The mechanisms underlying postoperative pain remain unclear. Neurotransmitters of excitatory and inhibitory amino acids play an important role in the transmission and modulation of pain in the spinal dorsal horn. This study aimed to investigate the changes of release of excitatory and inhibitory amino acids ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang Y,Yue Y,Shi L,Wu AS,Feng CS,Ni C

    更新日期:2007-11-20 00:00:00

  • Oral contrast agents lead to underestimation of dose calculation in volumetric-modulated arc therapy planning for pelvic irradiation.

    abstract:BACKGROUND:The effects of oral contrast agents (OCAs) on dosimetry have not been studied in detail. Therefore, this study aimed to examine the influence of OCAs on dose calculation in volumetric-modulated arc therapy plans for rectal cancer. METHODS:From 2008 to 2016, computed tomography (CT) images were obtained from...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000001025

    authors: Jing H,Tian Y,Tang Y,Wang SL,Jin J,Song YW,Liu YP,Fang H,Chen B,Qi SN,Tang Y,Lu NN,Yang Y,Li N,Li YX

    更新日期:2020-09-05 00:00:00

  • Inflammation unmasks gabapentin's effect on A delta-fiber evoked excitatory postsynaptic currents in substantia gelatinosa neurons of rat spinal cord.

    abstract:OBJECTIVE:To study the analgesic mechanism of gabapentin, an anticonvulsant, during antinociceptive clinical treatment. METHODS:Whole-cell voltage-clamp recordings were taken from adult rat spinal cord slices to investigate the effect of gabapentin on primary afferent A delta-fiber evoked excitatory postsynaptic curre...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Liu Z,Xu R,Yang K

    更新日期:2003-06-01 00:00:00

  • Trocar-site hernia after gynecological laparoscopic surgery: a 20-year, single-center experience.

    abstract:BACKGROUND:Trocar-site hernia (TSH) is a serious complication after laparoscopic procedures. Although it is a rare entity, it can have life-threatening consequences. This study aimed to retrospectively analyze the potential associated factors for TSH following gynecologic laparoscopy and summarize prevention strategies...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000000510

    authors: Zhu YP,Liang S,Zhu L,Sun ZJ,Lang JH

    更新日期:2019-11-20 00:00:00

  • Pathological demography of native patients in a nephrology center in China.

    abstract:OBJECTIVE:To analysis the pathological demography in Chinese patients undergoing renal biopsy from our nephrology center. METHODS:Between January 1979 and October 2000 in Jinling Hospital, Nanjing, China, 10,002 attempts of percutaneous renal were performed in patients with renal disease from 33 provinces of China. Th...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chen H,Tang Z,Zeng C,Hu W,Wang Q,Yu Y,Yao X,Wang J,Zhu M,Zhou H,Liu H,Liu Z,Li L

    更新日期:2003-09-01 00:00:00

  • Pulmonary endarterectomy for chronic thromboembolic pulmonary hypertension: preliminary exploration in China.

    abstract:BACKGROUND:Pulmonary endarterectomy is safe and effective surgical treatment for chronic thromboembolic pulmonary hypertension. This study aimed to evaluate the efficacy of pulmonary endarterectomy in treatment of thromboembolic pulmonary hypertension. METHODS:A retrospective study of 15 patients who underwent pulmona...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Gu S,Liu Y,Su PX,Zhai ZG,Yang YH,Wang C

    更新日期:2010-04-20 00:00:00

  • Intracoronary stent implantation under intracoronary ultrasound guidance with aspirin and ticlopidine therapy.

    abstract:OBJECTIVE:To observe the immediate angiographic and intravascular ultrasound (IVUS) results and their effects on one month clinical outcomes in forty-one patients who submitted to coronary stent deployment with IVUS guidance. METHODS:All patients were allocated to coronary stent implantation with high inflation pressu...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zhang D,Cai X,Shen W,Schiele F,Bassand JP

    更新日期:2001-03-01 00:00:00

  • Genetic variants in three genes and smoking show strong associations with susceptibility to exudative age-related macular degeneration in a Chinese population.

    abstract:BACKGROUND:The present study was undertaken to replicate the associations of representative polymorphisms in three genes (complement factor H (CFH), complement factor B (BF) and HtrA serine peptidase 1 (HTRA1)) with exudative age-related macular degeneration (AMD) in a Han Chinese population, and to test if the modifia...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chu J,Zhou CC,Lu N,Zhang X,Dong FT

    更新日期:2008-12-20 00:00:00

  • Generation and analysis of 113 adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags.

    abstract:OBJECTIVE:To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST). METHODS:A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags (ES...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Bian G,Yu X,Wu Z

    更新日期:2002-10-01 00:00:00

  • Effects of propofol, midazolam and thiopental sodium on outcome and amino acids accumulation in focal cerebral ischemia-reperfusion in rats.

    abstract:OBJECTIVE:To investigate the effects of propofol, midazolam and thiopental sodium on outcomes and amino acid accumulation in focal cerebral ischemia-reperfusion in rats. METHODS:Male Sprague Dawley (SD) rats were scheduled to undergo 3-hour middle cerebral artery occlusion by intraluminal suture and 24-hour reperfusio...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Chen L,Gong Q,Xiao C

    更新日期:2003-02-01 00:00:00