Abstract:
:This article reviews recent developments in methods of sample preparation and analytical methodologies for the quantification of anthocyanins and their extraction from food sources. Various methods for sample extraction and purification are highlighted and evaluated. The use of UV-diode array, along with improved liquid chromatography (LC) and mass spectrometry (MS) and/or the combination of both methods have facilitated the identification of analytes. The use of one-dimensional and two-dimensional HPLC has significantly improved resolution with a shorter amount of time. Other LC × LC combinations to improve orthogonality are also discussed. The most efficient anthocyanin extraction method from food sources is pressurized liquid extraction. Moreover, electrospray ionization (ESI) and MS2/time-of-flight are currently the most popular instruments used for identification of anthocyanins; being positive mode of ESI the most widely used procedure for anthocyanin identification. Several databases for mass spectrometry polyphenol identification have been described for reference.
journal_name
Food Chemjournal_title
Food chemistryauthors
Ongkowijoyo P,Luna-Vital DA,Gonzalez de Mejia Edoi
10.1016/j.foodchem.2018.01.055subject
Has Abstractpub_date
2018-06-01 00:00:00pages
113-126eissn
0308-8146issn
1873-7072pii
S0308-8146(18)30064-5journal_volume
250pub_type
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