当前位置： SCI文献检索 > Journal of Minimally Invasive Gynecology期刊下所有文献 > Of Mice and Women: A Laparoscopic Mouse Model for Endometriosis.

Of Mice and Women: A Laparoscopic Mouse Model for Endometriosis.

Abstract：

STUDY OBJECTIVE:To demonstrate how a novel laparoscopic approach allows the development of a mouse model for endometriosis after seeding menstrual endometrium from donor mice into the abdominal cavity of syngeneic recipient mice. DESIGN:A step-by-step video description of the techniques used to adapt the estrous cycle of mice towards a menstrual cycle and to subsequently induce endometriosis via laparoscopic seeding of menstrual endometrium. SETTING:University research institute. ETHICS:All animal experiments were ethically approved by KU Leuven, Belgium (ethical approval number: P031/2013). INTERVENTIONS, MEASUREMENTS, AND MAIN RESULTS:Oophorectomized female C57BL/6JRj mice received a series of estrogen injections. Next, a progesterone pellet was administered, together with a second series of estrogen injections. In addition, decidualization of the endometrium was induced with an intrauterine sesame oil stimulus. Four days later the progesterone pellet was removed and menstruation started [1]. Five hours after the progesterone pellet was removal the uterus was harvested, and the menstrual endometrium was dissected and seeded into the abdominal cavity of syngeneic recipient mice to induce endometriosis [2] using a laparoscopic approach [3]. Uterus and lesions were removed from the recipient mice 1 week after induction, and tissues were immunohistochemically stained for H&E, vimentin, and cytokeratin. CONCLUSION:In this video we show a novel methodology to induce endometriosis in mice using laparoscopic inoculation of syngeneic menstrual endometrium, mimicking Sampson's theory of retrograde menstruation [4]. Compared with currently available rodent models, our model offers a less invasive and more physiologic way for fundamental and preclinical endometriosis research, with a high endometriosis incidence and lesion take rate.

journal_name

J Minim Invasive Gynecol

journal_title

Journal of minimally invasive gynecology

authors

Peterse D,Binda MM,O DF,Vanhie A,Fassbender A,Vriens J,D'Hooghe TM

doi

10.1016/j.jmig.2017.10.008

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

578-579

issue

eissn

1553-4650

issn

1553-4669

pii

S1553-4650(17)31232-3

journal_volume

pub_type

杂志文章

在线工具

Of Mice and Women: A Laparoscopic Mouse Model for Endometriosis.