Antimicrobial effectiveness of combinations of oxidant and chelating agents in infected dentine: an ex vivo confocal laser scanning microscopy study.

Abstract:

AIM:To evaluate the intratubular antimicrobial activity of several oxidant and chelating agents associated with or without surfactants in experimentally infected root canals, using confocal laser scanning microscopy. METHODOLOGY:Twenty-four dentine blocks from bovine incisors were contaminated for 5 days with Enterococcus faecalis (ATCC- 29212). Ten contaminated dentine specimens were irrigated for 5 min with 5.25% NaOCl followed by 17% EDTA for 2 min, and the other 10 with Hypoclean for 5 min followed by Tetraclean NA for 2 min. The remaining four specimens were used as positive and negative controls (two samples each). Then, dentine blocks were stained with Live/Dead BacLight for analysis of the remaining live or dead bacteria using confocal laser scanning microscopy. Comparison between and within groups was performed using the Mann-Whitney test for independent samples and the Wilcoxon signed-rank test, respectively. RESULTS:After exposure to irrigants, the positive control group had a median of 67.41% of viable bacteria (95% CI: 48.15, 78.9), whilst the NaOCl+EDTA group and Hypoclean+Tetraclean NA group had 3.77% (1.28, 15.92) and 0.87% (-0.42, 4.30) of viable bacteria, respectively. These results were significantly different from each other, both overall and distinct by region (cervical and medium third), or depth (superficial and deep layer) (P < 0.01 in all cases). CONCLUSIONS:The use of adjunctive agents reducing the surface tension associated with oxidant and chelating agents improved the antimicrobial activity of irrigating solutions and intratubular decontamination against Enterococcus faecalis, possibly due to better removal of the smear layer and deeper penetration into dentinal tubules.

journal_name

Int Endod J

authors

Giardino L,Del Fabbro M,Cesario F,Fernandes FS,Andrade FB

doi

10.1111/iej.12863

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

448-456

issue

4

eissn

0143-2885

issn

1365-2591

journal_volume

51

pub_type

杂志文章
  • Endodontic-periodontal management of two rooted maxillary lateral incisor associated with complex radicular lingual groove by using spiral computed tomography as a diagnostic aid: a case report.

    abstract:AIM:To present the successful endodontic and periodontal management of a two rooted maxillary lateral incisor tooth with a complex radicular lingual groove and severe periodontal destruction using spiral computed tomography as a diagnostic aid. SUMMARY:A 30-year-old male patient presented with a chief complaint of mob...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2011.01868.x

    authors: Gandhi A,Kathuria A,Gandhi T

    更新日期:2011-06-01 00:00:00

  • Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 2. Root-canal-filling materials.

    abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2591.2003.00637.x

    authors: Hauman CH,Love RM

    更新日期:2003-03-01 00:00:00

  • Short-term tissue response to potential root-end filling materials in infected root canals.

    abstract::The short-term tissue responses to two potential root-end filling materials, a light-cured glass ionomer cement (Vitrebond) and a reinforced zinc oxide-eugenol cement (Kalzinol), were compared with that to amalgam using a previously devised experimental model. In 24 premolar teeth of beagle dogs (47 roots), a collecti...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1997.00077.x

    authors: Chong BS,Pitt Ford TR,Kariyawasam SP

    更新日期:1997-07-01 00:00:00

  • Effectiveness in cleaning oval-shaped root canals using Anatomic Endodontic Technology, ProFile and manual instrumentation: a scanning electron microscopic study.

    abstract:AIM:To compare in vitro the cleanliness of root canal walls in oval-shaped root canals following automated or manual instrumentation. METHODOLOGY:Forty-five oval-shaped single-rooted maxillary and mandibular premolars with straight canals were divided into three groups of 15. Automated canal preparation was performed ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2005.00938.x

    authors: Zmener O,Pameijer CH,Banegas G

    更新日期:2005-06-01 00:00:00

  • Thermal sensitivity of endodontically treated teeth.

    abstract:CASE REPORTS:The problem of thermal sensitivity following non-surgical root-canal treatment is explored and case reports are presented. Possible causes for post-treatment discomfort from endodontic and restorative aetiologies are discussed, as are the mechanisms to explain the patients' painful experiences. Treatment o...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1999.00197.x

    authors: Tidwell E,Witherspoon DE,Gutmann JL,Vreeland DL,Sweet PM

    更新日期:1999-03-01 00:00:00

  • Effect of delayed scanning on imaging and on the diagnostic accuracy of vertical root fractures in two photostimulable phosphor plates digital systems.

    abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12547

    authors: Nascimento HA,Visconti MA,Ferreira LM,Suarez MA,Haiter Neto F,Freitas DQ

    更新日期:2016-10-01 00:00:00

  • A comparative study of root canal preparation using FlexMaster and HERO 642 rotary Ni-Ti instruments.

    abstract:AIM:To compare several parameters of root canal preparation using two different rotary nickel-titanium (Ni-Ti) instruments: FlexMaster (VDW, Munich, Germany) and HERO 642 (Micro-Mega, Besançon, France). METHODOLOGY:Fifty extracted human mandibular molars with root canal curvatures between 20 and 40 degrees were embedd...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.2003.00664.x

    authors: Hülsmann M,Gressmann G,Schäfers F

    更新日期:2003-05-01 00:00:00

  • Failure characteristics of endodontically treated premolars restored with a post and direct restorative material.

    abstract::Ninety-one extracted maxillary premolar teeth were restored with a prefabricated post and amalgam, composite resin or glass-cermet core. Each group was again divided into three groups of 9-13 teeth to be subjected to an increasing load in one of three standardized directions (10, 45 and 90 degrees to the long axis of ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1992.tb00774.x

    authors: Huysmans MC,Peters MC,Plasschaert AJ,van der Varst PG

    更新日期:1992-05-01 00:00:00

  • Scanning electron microscope appearances of Lightspeed instruments used clinically: a pilot study.

    abstract::The cutting heads of the first 18 instruments in six sets of clinically used Lightspeed instruments (sizes 20-65) were examined in a scanning electron microscope (SEM). Three sets had been used by full-time faculty and discarded as follows: instrument sizes 20-32.5 after 18 treated root canals; instrument sizes 35-47....

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:

    authors: Marending M,Lutz F,Barbakow F

    更新日期:1998-01-01 00:00:00

  • Involvement of SDF-1 and monocyte chemoattractant protein-1 in hydrogen peroxide-induced extracellular matrix degradation in human dental pulp cells.

    abstract:AIM:To determine whether chemokines such as SDF-1 and monocyte chemoattractant protein-1 (MCP-1) are responsible for hydrogen peroxide (H2 O2 )-induced extracellular matrix (ECM) degradation and to identify the underlying mechanism in human dental pulp cells (HDPCs). METHOD:Human dental pulp cells were exposed to 0.4 ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12147

    authors: Kim DS,Kang SI,Lee SY,Noh KT,Kim EC

    更新日期:2014-03-01 00:00:00

  • Biocompatibility of root filling pastes used in primary teeth.

    abstract:AIM:To evaluate the biocompatibility of two pastes designed to fill the root canals of primary teeth. METHODOLOGY:A study group of 54 mice received subcutaneous tissue implants of polyethylene tubes containing CTZ or calcium hydroxide paste or, as a negative control, empty tubes. Biocompatibility was evaluated on days...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12328

    authors: Lima CC,Conde Júnior AM,Rizzo MS,Moura RD,Moura MS,Lima MD,Moura LF

    更新日期:2015-05-01 00:00:00

  • Effect of oral premedication on the anaesthetic efficacy of inferior alveolar nerve block in patients with irreversible pulpitis - A systematic review and network meta-analysis of randomized controlled trials.

    abstract::This systematic review (SR; PROSPERO database: CRD42017075160) and network meta-analysis (NMA) identified the most effective oral premedication for anaesthetic success of inferior alveolar nerve blocks (IANB) in cases of irreversible pulpitis. Medline and Ebscohost databases were searched up until 10/2017. Randomized ...

    journal_title:International endodontic journal

    pub_type: 杂志文章,meta分析

    doi:10.1111/iej.12912

    authors: Pulikkotil SJ,Nagendrababu V,Veettil SK,Jinatongthai P,Setzer FC

    更新日期:2018-09-01 00:00:00

  • Dentine sialoprotein expression in gingival crevicular fluid during trauma-induced root resorption.

    abstract:AIM:To detect and quantify dentine sialoprotein (DSP) in the gingival crevicular fluid (GCF) of luxated teeth. METHODOLOGY:Eighteen subjects were enroled and distributed as follows. Group I (n = 6, positive control): subjects with primary second molar teeth undergoing physiological root resorption. Group II (n = 6, ne...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12001

    authors: Kumar V,Logani A,Shah N

    更新日期:2013-04-01 00:00:00

  • Incidence of root fractures and methods used for post removal.

    abstract:AIM:This study was undertaken to determine the incidence of root fractures during post removal, as well as the methods used and the success rates achieved with various post removal devices and techniques. METHODOLOGY:Patient records from a specialist endodontic practice were examined. A total of 1600 teeth from which ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.2002.00457.x

    authors: Abbott PV

    更新日期:2002-01-01 00:00:00

  • Comparison of centring ability and transportation between four nickel titanium instrumentation techniques by micro-computed tomography.

    abstract:AIM:To compare the centring ability and transportation of ProTaper Next (PTN), ProTaper Universal (PTU), Race 123 and RevoS using micro-computed tomography (μCT). METHODOLOGY:Sixty mesial root canals of thirty mandibular molars were divided virtually into coronal, middle and apical thirds, and two reproducible referen...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12658

    authors: Saberi N,Patel S,Mannocci F

    更新日期:2017-06-01 00:00:00

  • Antimicrobial 2-hydroxyisocaproic acid and chlorhexidine resist inactivation by dentine.

    abstract:AIM:To compare the antibacterial activity of 2-hydroxyisocaproic acid (HICA) with currently used root canal medicaments and to examine their interactions with potential inhibitors in nutrient-deficient and nutrient-rich conditions. METHODOLOGY:First, the antibacterial activity of single concentrations of HICA, calcium...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12465

    authors: Sakko M,Tjäderhane L,Sorsa T,Hietala P,Rautemaa R

    更新日期:2016-04-01 00:00:00

  • Comparison of two vertical condensation obturation techniques: Touch 'n Heat modified and System B.

    abstract:AIM:The aims of this study were firstly to compare the area of canal occupied by gutta-percha, sealer or voids using the System B heating device with that obtained by a modified vertical condensation technique using the Touch 'n Heat: and secondly to compare the temperature changes at the root canal wall and external r...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1999.00215.x

    authors: Silver GK,Love RM,Purton DG

    更新日期:1999-08-01 00:00:00

  • Micro-CT analysis of chronic apical periodontitis induced by several specific pathogens.

    abstract:AIM:To conduct three-dimensional micro-CT analysis of bone destruction, periapical sclerotic changes and inflammatory root resorption (IRR) to compare the differences between Enterococcus faecalis (Ef)- and Porphyromonas gingivalis (Pg)-induced chronic apical periodontitis (CAP). METHODOLOGY:Mono-species bacteria-indu...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13095

    authors: Chen S,Lei H,Luo Y,Jiang S,Zhang M,Lv H,Cai Z,Huang X

    更新日期:2019-07-01 00:00:00

  • Variation in the microleakage produced by four different techniques in root fillings in a simulated root canal model.

    abstract::An in-vitro investigation was performed to assess the extent of apical microleakage in simulated canals in clear resin blocks. Four different methods of producing dye penetration were employed: passive dye penetration alone, centrifugation alone, vacuum plus passive dye penetration, and increased pressure plus passive...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1996.tb01362.x

    authors: Pathomvanich S,Edmunds DH

    更新日期:1996-05-01 00:00:00

  • Behaviour of human dental pulp cells cultured in a collagen hydrogel scaffold cross-linked with cinnamaldehyde.

    abstract:AIM:To investigate the effects of the cross-linking agent cinnamaldehyde (CA) on differentiation of human dental pulp cells (hDPCs) cultured in a collagen hydrogel, which may be useful as a scaffold for regenerative endodontic therapy. METHODOLOGY:The odontogenic potential of hDPCs exposed to CA was examined using alk...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12592

    authors: Kwon YS,Lee SH,Hwang YC,Rosa V,Lee KW,Min KS

    更新日期:2017-01-01 00:00:00

  • Effect of bleaching agents on bonding to pulp chamber dentine.

    abstract:AIM:To determine the effect of intracoronal bleaching agents on adhesion of bonding agents to pulp chamber dentine. METHODOLOGY:Forty extracted human maxillary anterior teeth were randomly divided into four groups of 10 teeth each. Bleaching agents were sealed in pulp chambers for 7 days, as in clinical use. Group 1 (...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2004.00931.x

    authors: Timpawat S,Nipattamanon C,Kijsamanmith K,Messer HH

    更新日期:2005-04-01 00:00:00

  • Tissue responses to Hydron, assessed by intraosseous implantation.

    abstract::Teflon tubes containing freshly mixed, polymerized Hydron root canal filling material, fully set AH26 or Teflon were implanted into the mandible of guinea pigs and assessed histologically at 2 days, 1, 2, 4, 12 and 26 weeks. None of the materials tested elicited signs of overt or significant tissue damage, and polymer...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1992.tb00749.x

    authors: Reid RJ,Wilson DF,Chau KK,Heithersay GS,Heijkoop PS

    更新日期:1992-07-01 00:00:00

  • Comparative evaluation of antimicrobial efficacy of Syzygium aromaticum, Ocimum sanctum and Cinnamomum zeylanicum plant extracts against Enterococcus faecalis: a preliminary study.

    abstract:AIM:To evaluate the antimicrobial efficacy of Ocimum sanctum, Cinnamomum zeylanicum, Syzygium aromaticum and 3% sodium hypochlorite (NaOCl) against Enterococcus faecalis in planktonic suspension and biofilm phenotypes. METHODOLOGY:The antibacterial efficacy of different concentrations of aqueous ethanolic extracts of ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12058

    authors: Gupta A,Duhan J,Tewari S,Sangwan P,Yadav A,Singh G,Juneja R,Saini H

    更新日期:2013-08-01 00:00:00

  • A simple mounting model for consistent determination of the accuracy and repeatability of apex locators.

    abstract:AIM:To develop a precise and simple mounting model (MM) for evaluating apex locators and to compare the repeatability of the MM with a conventional visual method (VM). METHODOLOGY:Electronic working length determination was performed in 32 maxillary central incisors using two methods: (i) the MM method and (ii) a conv...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2006.01052.x

    authors: ElAyouti A,Löst C

    更新日期:2006-02-01 00:00:00

  • Focal cemento-osseous dysplasia involving a mandibular lateral incisor.

    abstract:AIM:To report a case of focal cemento-osseous dysplasia (FCOD) affecting a single tooth misdiagnosed as an inflammatory periapical lesion. SUMMARY:The patient, a black 47-year-old woman complained of pain affecting the right side of the mandible. Routine X-ray examination discovered a periapical radiolucency on the ma...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2003.00736.x

    authors: Galgano C,Samson J,Küffer R,Lombardi T

    更新日期:2003-12-01 00:00:00

  • Effect of root canal irrigation protocols on the dislocation resistance of mineral trioxide aggregate-based materials: A systematic review of laboratory studies.

    abstract::The aim of this systematic review was to address the question: Do different irrigating protocols have an impact on the dislocation resistance of mineral trioxide aggregate (MTA)-based materials? The review was performed using a well-defined search strategy in three databases (PubMed, Scopus, Web of Science) to include...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1111/iej.12898

    authors: Neelakantan P,Ahmed HMA,Wong MCM,Matinlinna JP,Cheung GSP

    更新日期:2018-08-01 00:00:00

  • Comparative evaluation of the shaping ability of ProTaper Next, iRaCe and Hyflex CM rotary NiTi files in severely curved root canals.

    abstract:AIM:To compare the shaping ability of ProTaper Next, iRaCe and Hyflex CM rotary NiTi files during the preparation of severely curved root canals in extracted human molar teeth. METHODOLOGY:Sixty mandibular molars with mesio-buccal canals having angles of curvature ranging from 25° to 35° were divided according to the ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12291

    authors: Saber SE,Nagy MM,Schäfer E

    更新日期:2015-02-01 00:00:00

  • A tactile method for canal length determination in teeth with open apices.

    abstract:AIM:To present a tactile method for working length determination in teeth with open apices and to determine its accuracy and repeatability. METHODOLOGY:Ninety teeth with 129 root canals were prepared to create open apices. The correct working length (CWL) for each canal was determined by introducing a file into the ro...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2009.01636.x

    authors: ElAyouti A,Dima E,Löst C

    更新日期:2009-12-01 00:00:00

  • Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain.

    abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...

    journal_title:International endodontic journal

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1365-2591.2001.00357.x

    authors: Sim TP,Knowles JC,Ng YL,Shelton J,Gulabivala K

    更新日期:2001-03-01 00:00:00

  • Digital radiographs displayed on different devices: effect on the detection of vertical root fractures.

    abstract:AIM:To evaluate whether the type of display device affects the detection of vertical root fractures (VRFs) on digital radiographs in unfilled canals and canals with fibreglass posts. METHODOLOGY:Forty single-rooted human teeth were decoronated, and the root canals were prepared. The teeth were divided into 2 groups: c...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12466

    authors: Vasconcelos TV,Santaella GM,Nascimento HA,Rovaris K,Ambrosano GM,Freitas DQ

    更新日期:2016-04-01 00:00:00