Abstract：
BACKGROUND:Isotopically labeled relative and absolute quantification (iTRAQ) were applied together with liquid chromatogram-tandem mass spectrometry (LC-MS/MS) technique to screen and differentiate normal tissues and differential proteins of placenta tissue in the pre-eclampsia group, and to seek for a pre-eclampsia biomarker. METHODS:Thirty patients with severe pre-eclampsia (pre-eclampsia group) and 30 patients with normal full-term pregnancy (control group), who were admitted by the Obstetrical Department of The Affiliated Hospital of Qingdao University from December 2014 to June 2015, were collected for this study. Part of the placenta tissue was sampled following cesarean section to extract the total protein that was degenerated, reduced and enzymatically hydrolyzed. Following iTRAQ, mass spectroscopy was used for differentiation to obtain differential proteins in the expression. RESULTS:A total of 234 differential proteins were identified. The comparison between the severe pre-eclampsia group and the control group indicated over 1.5-folds of expression abundance difference (upregulation ratio >1.50, or downregulation ratio <0.67). The difference was statistically significant in 24 protein points in total, among which the expression of 14 protein points was upregulated compared with that of the control group, and the expression of 10 protein points was downregulated. CONCLUSIONS:iTRAQ combined with the LC-MS/MS technique can effectively screen differential proteins in placenta tissues of patients with pre-eclampsia.
journal_name
Minerva Medjournal_title
Minerva medicaauthors
Qi WH,Zheng MY,Li C,Xu L,Xu JEdoi
10.23736/S0026-4806.17.05080-7subject
Has Abstractpub_date
2017-10-01 00:00:00pages
389-395issue
5eissn
0026-4806issn
1827-1669pii
S0026-4806.17.05080-7journal_volume
108pub_type
杂志文章相关文献
MINERVA MEDICA文献大全abstract：:The aim of the study was to evaluate the clinical efficacy of the association of heparin and sulodexide on experimental pain and on "small sport traumatology". In the experimental part of the study, cutaneous pain threshold and subcutaneous tissue ticking were measured after the induction of an algogenic focus in deep...
journal_title：Minerva medica
pub_type： 临床试验,杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Calm breathing requires little effort on the part of the inspiratory muscles to overcome the elastic and non-elastic resistances of the chest-lung system. Spontaneous expiration is passive and does not engage the respiratory muscles. In the event of restriction or obstruction, however, there are changes in these two t...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:The Monfalcone area (Italy) is a small industrial territory (60,000 inhabitants), characterized by a high incidence of asbestos-related diseases. In a review of the biopsy and necropsy records collected at the Monfalcone Hospital during the period October 1979-September 1985 two cases of Kaposi's sarcoma were found. O...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1986-06-23 00:00:00
abstract：:A group of 104 workers were examined. They were employed in selecting rags and separating the lining from wollen fabrics to be used again as thread waste in the textile industry. The aim of the study was to point out tendon and joint related disorders of the upper limb due to repetitive and forced movements. Twenty-ei...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1995-11-01 00:00:00
abstract：:The combination acetominophen/tramadol implies the use of two molecules which are different in the pharmacological structure but share a biological similarity in kinetics. Their respective actions are directed to different components of the nociceptive afference. Hematological clinical practice daily challenges diseas...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：2008-12-01 00:00:00
abstract：:The term therapeutic endoscopic ultrasound (EUS) refers to direct ultrasound guided placement of pharmaceuticals, devices and biological agents for the purpose of treating a disease or symptoms. The vast majority of EUS procedures in the world are performed for diagnostic purposes, although these lead directly to ther...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：2007-08-01 00:00:00
abstract：:A rare case of skin tumour, Merkel cell carcinoma, is reported. The histopathological and clinical features and surgical treatment are reported. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:Data relating to the diffusion of venereal diseases in the Fiuli and Venezia-Giulia region over the period 1946-71 are presented. The picture is much the same as that observed elsewhere in Italy and in other European and non-European countries. This was more true of the provinces of Trieste and Gorizia than those of U...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1975-04-25 00:00:00
abstract：:Aims of our study were: to evaluate small airway function of subjects with past or present silica dust exposure and normal spirometric values; to investigate whether small airway disease is related to radiographic signs of silicosis, to cumulative dust exposure (ES) and to cigarette smoking. Maximal expiratory flow at...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1986-11-30 00:00:00
abstract：: ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：10.23736/S0026-4806.19.06251-7
更新日期：2019-09-27 00:00:00
abstract：:In the light of the international literature and personal observation, the diagnostic techniques employed in Pneumocystis Carinii Pneumonia (PCP), a major opportunistic infection of acquired immunodeficiency syndrome (AIDS) are reviewed. ...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1988-06-01 00:00:00
abstract：:Current estimates of the prevalence of chronic pancreatitis, one of the most common causes of exocrine pancreatic insufficiency, are in the range of 3-10 per 100,000 people in many parts of the world. Alcohol consumption is a very important risk factor for exocrine pancreatic insufficiency and is involved in nearly ha...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：10.23736/S0026-4806.19.06043-9
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND:The frequency of advanced non-small cell lung cancer (NSCLC) increases with age and more effective and less toxic chemotherapy schedules are needed in elderly patients. Cisplatin-based regimens are considered the best treatment for advanced NSCLC, although they produce only a modest advantage in overall surv...
journal_title：Minerva medica
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Multivaried and trend analyses were applied to a set of 27 periodic haematochemical checks on 37 patients given surgery for biliopancreatic pathology. Preoperative and postoperative data were separately analysed. The software employed was specially designed for the purpose. The discriminating function was used in the ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1983-02-25 00:00:00
abstract：:In this review, the Authors analyzes the more recent data in the Literature about the major cardiovascular risks in diabetic patients affected by systemic arterial hypertension. The more effective pharmacological and not pharmacological treatment is suggested. Particularly the advantages and more important side-effect...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1992-11-01 00:00:00
abstract：:Patients with peripheral arterial disease (PAD) are at increased risk for all-cause mortality, cardiovascular mortality, and mortality from coronary artery disease. Smoking should be stopped and hypertension, dyslipidemia, diabetes mellitus, and hypothyroidism treated. Statins decrease the incidence of intermittent cl...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:Colorectal cancer (CRC) is one of the most common carcinomas throughout the world. Although the diagnostic and therapeutic strategies have made some progression in the treatment of CRC patients, the mortality of CRC remains relatively high. Hence, it is an urgency to find out the detailed mechanisms of how C...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：:The clinical polymorphism of brucellosis is well known fact. It is thought personally that respiratory system localizations are more frequent than have so far been reported in the literature and serious differential diagnosis problems arise with tuberculosis, neoplasia and pneumoconiosis. After discussing such problem...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1977-05-09 00:00:00
abstract：:Birth of an infant at term to a diabetic woman with a severe form of Friedreich's disease is reported. The aetiopathogenesis of the cardiac deformities that led to the death of the child on day seven of life is discussed. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1976-02-11 00:00:00
abstract：:103 patient suffering from thyroid solitary nodule have been examined with gray-scale ultrasonography. 79 patients have been histologically confirmed. Research has been done with multi-transducer computerized water-bath equipment ( OCTOSON ). The results are discussed and a classification of the thyroid solitary nodul...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1984-05-12 00:00:00
abstract：:Personal experience of pacemakers in patients suffering from (symptomatic and asymptomatic) 2nd degree AVB and 3rd degree AVB is reported. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:40 hyperlipidaemic patients (26 type II a and b, 14 type IV) and 50 healthy controls were studied. The patients were not suffering from any other pathology of note and were under no drug treatment. Blood viscosity at natural Ht and at Ht 45% and plasma viscosity were measured. Red blood cell viscosity and deformabilit...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1987-06-30 00:00:00
abstract：:The object of the present study is to review all that in the last years has been discovered about growth hormone-releasing hormone (GHRH), in order to point out both its physiopathological characteristics and its possible diagnostic and therapeutic use. In the first section are summarily reviewed the different studies...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1989-04-01 00:00:00
abstract：:Buckley's syndrome is a nosologic entity characterized by the presence from birth of extremely elevated levels of circulating immunoglobulin E and an important deficiency of polymorphonuclear chemotaxis. The consequences of such alterations are represented by the premature rising of recurrent cutaneous, pulmonary and ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:The synthetic mucolytic neltene-xine is an amide derivative of ambroxol and thiophencarboxylic acid. The aim of this open, randomised, controlled study versus sobrerol was to evaluate the efficacy and tolerability of neltenexine (oral granules in sachets) as compared with sobrerol (oral granules in sachets),...
journal_title：Minerva medica
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2001-08-01 00:00:00
abstract：AIM:Adequate postoperative analgesia is a prerequisite for successful ambulatory surgery and continues to be a challenge for anesthesiologists. The goal of the study was to analyze what are determinants of patients' overall satisfaction in postoperative pain management after ambulatory hand surgery. METHODS:Patients u...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：:Lung transplant is a therapeutic option for end stage lung diseases, but only a limited number of lung grafts is considered suitable for transplantation. It has been recently suggested an approach to improve and maximize donor lung suitability, namely ventilation strategies to prevent lung damage and preserve function...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：2016-06-01 00:00:00
abstract：:Idiopathic hypereosinophilic syndrome is a term labelling clinical illnesses characterized by blood hypereosinophilia and widespread infiltration of organs and tissues by mature eosinophils. Although any tissue can be affected the involvement of the nervous system and of the myocardium usually indicates worse prognosi...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1985-10-13 00:00:00
abstract：:A patient with severe Henoch-Schoenlein purpura was successfully treated by combined plasmapheresis and cyclophosphamide therapy. PE proved to be effective in the symptomatic treatment of the disease. Its possible role in the prevention and treatment of HS nephritis is suggested. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1982-04-28 00:00:00
abstract：:The occurrence of two new cancers (a diffuse centrocytic lymphoma and a basal cell cancer) in a patient successfully treated for Hodgkin's disease 29 years before is reported. The centrocytic lymphoma mainly involved the skin, the basal cell carcinoma developed on the dorsum of the right hand. The patient had received...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
