Abstract:
:Stress induces changes of modified nucleosides in tRNA, and these changes can influence codon-anticodon interaction and therefore the translation of target proteins. Certain nucleoside modification genes are associated with regulation of stress tolerance and immune response in plants. In this study, we found a dramatic increase of 2'-O-methyladenosine (Am) nucleoside in rice seedlings subjected to salt stress and abscisic acid (ABA) treatment. We identified LOC_Os03g61750 (OsTRM13) as a rice candidate methyltransferase for the Am modification. OsTRM13 transcript levels increased significantly upon salt stress and ABA treatment, and the OsTrm13 protein was found to be located primarily to the nucleus. More importantly, OsTRM13 overexpression plants displayed improved salt stress tolerance, and vice versa, OsTRM13 RNA interference (RNAi) plants showed reduced tolerance. Furthermore, OsTRM13 complemented a yeast trm13Δ mutant, deficient in Am synthesis, and the purified OsTrm13 protein catalysed Am nucleoside formation on tRNA-Gly-GCC in vitro. Our results show that OsTRM13, encoding a rice tRNA nucleoside methyltransferase, is an important regulator of salt stress tolerance in rice.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Wang Y,Li D,Gao J,Li X,Zhang R,Jin X,Hu Z,Zheng B,Persson S,Chen Pdoi
10.1093/jxb/erx061subject
Has Abstractpub_date
2017-03-01 00:00:00pages
1479-1491issue
7eissn
0022-0957issn
1460-2431pii
3091893journal_volume
68pub_type
杂志文章abstract::Sexual reproduction in higher plants uses pollination, involving interactions between pollen and pistil. Self-incompatibility (SI) prevents self-fertilization, providing an important mechanism to promote outbreeding. SI is controlled by the S-locus; discrimination occurs between incompatible pollen, which is rejected,...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm195
更新日期:2008-01-01 00:00:00
abstract::In the present study abscisic acid-induced stomatal closing, and malate effects on stomatal apertures were analysed in the presence of guard cell ion channel regulators. A recent study has suggested that abscisic acid (ABA) activation of protein kinases and/or inhibition of protein phosphatases may be central to activ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/48.Special_Issue.539
更新日期:1997-03-01 00:00:00
abstract::Low-temperature stress is an important factor affecting the growth and development of rice (Oryza sativa L.) in temperate and high-elevation areas. Cold stress may cause various seedling injuries, delayed heading and yield reduction due to spikelet sterility. In this study, 181 microsatellite marker loci were used to ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg243
更新日期:2003-11-01 00:00:00
abstract::Citric acid is the predominant organic acid of citrus fruit. Degradation of citric acid occurs during fruit development, influencing fruit acidity. Associations of CitAco3 transcripts and citric acid degradation have been reported for citrus fruit. Here, transient overexpression of CitAco3 significantly reduced the ci...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx187
更新日期:2017-06-15 00:00:00
abstract::While marine phytoplankton rival plants in their contribution to global primary productivity, our understanding of their photosynthesis remains rudimentary. In particular, the kinetic diversity of the CO2-fixing enzyme, Rubisco, in phytoplankton remains unknown. Here we quantify the maximum rates of carboxylation (k c...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw163
更新日期:2016-05-01 00:00:00
abstract::Potassium (K+) is the major cationic inorganic nutrient utilized for osmotic regulation, cell growth, and enzyme activation in plants. Inwardly rectifying K+ channel 1 (AKT1) is the primary channel for root K+ uptake in plants, but the function of HvAKT1 in barley plants under drought stress has not been fully elucida...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa354
更新日期:2020-10-22 00:00:00
abstract::In eukaryotes, the RHO superfamily of small G-proteins is implicated in the regulation of cell polarity and growth. Rho of Plants (ROPs)/RACs are plant-specific Rho family proteins that have been shown to regulate cell polarity, auxin transport and responses, ABA signalling, and response to pathogens. A hallmark of RO...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv057
更新日期:2015-03-01 00:00:00
abstract::Both tracheary elements and fiber cells undergo programmed cell death (PCD) during xylem development. In this study we investigated the role of papain-like cysteine protease CEP1 in PCD in the xylem of Arabidopsis. CEP1 was located in the cell wall of xylem cells, and CEP1 expression levels in inflorescence stems incr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery356
更新日期:2019-01-01 00:00:00
abstract::Maintenance of a functional root is fundamental to plant survival in response to some abiotic stresses, such as water deficit. In this study, we found that overexpression of Arabidopsis class 1 phytoglobin (AtPgb1) alleviated the growth retardation of polyethylene glycol (PEG)-induced water stress by reducing programm...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx371
更新日期:2017-11-28 00:00:00
abstract::Auxin is an essential regulator of plant growth and development, and auxin signaling components are conserved among land plants. Yet, a remarkable degree of natural variation in physiological and transcriptional auxin responses has been described among Arabidopsis thaliana accessions. As intraspecies comparisons offer...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw457
更新日期:2017-01-01 00:00:00
abstract::C4 photosynthesis involves a close collaboration of the C3 and C4 metabolic cycles across the mesophyll and bundle-sheath cells. This study investigated the coordination of C4 photosynthesis in maize plants subjected to two salinity (50 and 100mM NaCl) treatments and one shade (20% of full sunlight) treatment. Photosy...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru130
更新日期:2014-07-01 00:00:00
abstract::Rapid alkalinization factor (RALF) is a peptide signal that plays a basic role in cell biology and most likely regulates cell expansion. In this study, transgenic Arabidopsis thaliana lines with high and low levels of AtRALF1 transcripts were used to investigate this peptide's mechanism of action. Overexpression of th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru099
更新日期:2014-05-01 00:00:00
abstract::Recent studies have suggested that aluminium (Al) induces programmed cell death (PCD) in plants. To investigate possible mechanisms, fluorescence techniques were used to monitor the behaviour of mitochondria in vivo, as well as the activation of caspase-3-like activity during protoplast PCD induced by Al. A quick burs...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq279
更新日期:2011-01-01 00:00:00
abstract::The history of tomato (Solanum lycopersicum L.) improvement includes genetic bottlenecks, wild species introgressions, and divergence into distinct market classes. This history makes tomato an excellent model to investigate the effects of selection on genome variation. A combination of linkage mapping in two F(2) popu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq367
更新日期:2011-03-01 00:00:00
abstract::When plants develop, cell proliferation and cell expansion are tightly controlled in order to generate organs with a determinate final size such as leaves. Several studies have demonstrated the importance of the cell proliferation phase for leaf growth, illustrating that cell-cycle regulation is crucial for correct le...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert388
更新日期:2014-06-01 00:00:00
abstract::Polarized growth of pollen tubes is a critical step for successful reproduction in angiosperms and is controlled by ROP GTPases. Spatiotemporal activation of ROP (Rho GTPases of plants) necessitates a complex and sophisticated regulatory system, in which guanine nucleotide exchange factors (RopGEFs) are key components...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert323
更新日期:2013-12-01 00:00:00
abstract::The spatial and temporal distribution of expression of two cytosolic members of the AtHsp90 gene family was assessed during early development. In stressed transgenic plants bearing the AtHsp90-3 promoter, beta-glucuronidase (GUS) activity was strong in meristematic tissues. Expression was also detected in vascular tis...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri035
更新日期:2005-02-01 00:00:00
abstract::In grapevine, stimulation of defence responses was evidenced in response to various types of abiotic stresses in both leaves and berries, as revealed by the increasing expression of genes encoding defence-related proteins or the stimulation of their corresponding activities. However, the capability of inflorescences t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern361
更新日期:2009-01-01 00:00:00
abstract::Growing potato tubers or freshly harvested mature tubers have a dormant apical bud. Normally, this dormancy is spontaneously broken after a period of maturation of the tuber, resulting in the growth of a new sprout. Here it is shown that in in vitro-cultured growing and maturing tubers, ethanol can rapidly break this ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri245
更新日期:2005-09-01 00:00:00
abstract::Topoisomerase I (topo I) is a nuclear enzyme which plays a fundamental role in several pathways involving changes in DNA topology. The topo I-mediated reaction is accomplished by the transient covalent binding of the enzyme to DNA (topo I-DNA complex). Stabilization of the topo I-DNA complex, leading to irreversible d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj194
更新日期:2006-01-01 00:00:00
abstract::The composition of carotenoids, along with anthocyanins and chlorophyll, accounts for the distinctive range of colour found in the Actinidia (kiwifruit) species. Lutein and beta-carotene are the most abundant carotenoids found during fruit development, with beta-carotene concentration increasing rapidly during fruit m...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp218
更新日期:2009-01-01 00:00:00
abstract::The relative role of the maternal source and the filial sink in controlling the composition of maize (Zea mays L.) kernels is unclear and may be influenced by the genotype and the N supply. The objective of this study was to determine the influence of assimilate supply from the vegetative source and utilization of ass...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp324
更新日期:2010-01-01 00:00:00
abstract::The hydrophobic cuticular membrane of land plants performs a number of important roles during fruit development, including protection from a range of abiotic and biotic stresses. The components of the fleshy fruit cuticle are synthesized and secreted from the epidermal cells. While the biosynthetic and transport pathw...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru225
更新日期:2014-08-01 00:00:00
abstract::Coffee (Coffea spp.), a globally traded commodity, is a slow-growing tropical tree species that displays an improved photosynthetic performance when grown under elevated atmospheric CO2 concentrations ([CO2]). To investigate the mechanisms underlying this response, two commercial coffee cultivars (Catuaí and Obatã) we...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv463
更新日期:2016-01-01 00:00:00
abstract::The efficient exclusion of excess Na from the cytoplasm and vacuolar Na(+) accumulation are the main mechanisms for the adaptation of plants to salt stress. This is typically carried out by transmembrane transport proteins that exclude Na(+) from the cytosol in exchange for H(+), a secondary transport process which is...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp011
更新日期:2009-01-01 00:00:00
abstract::Oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the regulation of abscisic acid (ABA) synthesis in higher plants. ABA has been associated with dormancy and flower senescence, while also regulating plant adaptive responses to various environmental stresses....
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err293
更新日期:2012-01-01 00:00:00
abstract::The nucleus contains the cell's genetic material, which directs cellular activity via gene regulation. The physical barrier of the nuclear envelope needs to be permeable to a variety of macromolecules and signals. The most prominent gateways for the transport of macromolecules are the nuclear pore complexes (NPCs). Th...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ers258
更新日期:2013-02-01 00:00:00
abstract::Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert006
更新日期:2013-02-01 00:00:00
abstract::It has been suggested that antioxidants play a role in regulating or modulating senescence dynamics of plant tissues. Ethylene has been shown to promote early plant senescence while controlled atmospheres (CA; reduced O2 levels and elevated CO2 levels) can delay its onset and/or severity. In order to examine the possi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.344.645
更新日期:2000-03-01 00:00:00
abstract::With the aim of increasing the cysteine level in rice (Oryza sativa L.) and thus improving its nutritional quality, transgenic rice plants were generated expressing an Escherichia coli serine acetyltransferase isoform (EcSAT), the enzyme synthesizing O-acetylserine, the precursor of cysteine. The gene was fused to the...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers253
更新日期:2012-10-01 00:00:00