Abstract:
:An expression vector containing the murine c-ets-2 protooncogene cDNA was introduced into NIH 3T3 cells by DNA transfection. The cells transfected with this construct showed foci of densely growing, morphologically altered cells, when grown either in low-serum (0.05%) or in serum-free medium. The c-ets-2-derived foci contained additional copies of the c-ets-2 gene, Northern blot analysis demonstrated overexpression of a c-ets-2-specific 2.5-kilobase RNA, and ets-specific antiserum recognized a 56-kDa protein. Overexpression of the c-ets-2-encoded protein stimulated cell proliferation and abolished their serum requirement. The c-ets-2 transfected cells formed colonies in semisolid medium and induced tumors in nude mice, indicating that c-ets-2 can be a transforming gene when overexpressed in these cells. This work demonstrates that a member of the c-ets gene family has transforming and mitogenic activity. In addition, the role of c-ets-2 in cell proliferation and its location in the minimal Down syndrome region on chromosome 21 implicates its involvement in the phenotypic changes associated with Down syndrome.
journal_name
Proc Natl Acad Sci U S Aauthors
Seth A,Watson DK,Blair DG,Papas TSdoi
10.1073/pnas.86.20.7833subject
Has Abstractpub_date
1989-10-01 00:00:00pages
7833-7issue
20eissn
0027-8424issn
1091-6490journal_volume
86pub_type
杂志文章abstract::Array technologies have made it straightforward to monitor simultaneously the expression pattern of thousands of genes. The challenge now is to interpret such massive data sets. The first step is to extract the fundamental patterns of gene expression inherent in the data. This paper describes the application of self-o...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1999-03-16 00:00:00
abstract::Vaccination is the most effective medical intervention ever introduced and, together with clean water and sanitation, it has eliminated a large part of the infectious diseases that once killed millions of people. A recent study concluded that since 1924 in the United States alone, vaccines have prevented 40 million ca...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2014-08-26 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1997-04-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2011-10-25 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1987-08-01 00:00:00
abstract::In the retina, dopamine plays a central role in neural adaptation to light. Progress in the study of dopaminergic amacrine (DA) cells has been limited because they are very few (450 in each mouse retina, 0.005% of retinal neurons). Here, we applied transgenic technology, single-cell global mRNA amplification, and cDNA...
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更新日期:2004-04-06 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:1993-08-15 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2020-05-26 00:00:00
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更新日期:2009-10-20 00:00:00
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更新日期:1994-06-21 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1998-03-17 00:00:00
abstract::Increasing concentrations of estradiol (E2) ranging from 0.01 to 10 nM were found to inhibit partially but significantly the lactogenic effect of ovine prolactin (oPRL) on alpha-lactalbumin production in primate mammary tissues maintained in organ culture for 9 days. E2 at 10 nM inhibited by 38% (mean) PRL-stimulated ...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2005-06-28 00:00:00