Regulation of podocyte lesions in diabetic nephropathy via miR-34a in the Notch signaling pathway.

Abstract:

BACKGROUND:The activation of the Notch signaling pathway has been shown to play an important role in diabetic nephropathy (DN) development. Besides, Notch-1 is a target gene in miR-34a. However, the regulation of the podocyte lesions involved in DN by miR-34a has not been identified. METHODS:This study utilized miR-34a mimics and small interfering RNA transfection to construct miR-34a overexpression and lower-expression model to investigate the effect of miR-34a on the regulation of the Notch signaling pathway and podocyte lesions in DN. Western blotting and real-time quantitative polymerase chain reaction were applied for the quantitative testing of mRNA and protein expression. Apoptosis of podocyte was detected by TUNEL staining. RESULTS:In high-glucose (HG) conditions, miR-34a overexpression inhibited the expression of Notch 1, Jagged 1, NICD, Hes 1, and Hey 1 proteins. Further, cleaved caspase-3, Bax, and phosphorylation of p53 (p-p53) were reduced significantly. Therefore, miR-34a overexpression inhibited the Notch signaling pathway and podocyte lesions induced by HG. β-arrestin was slightly reduced in HG conditions. Meanwhile, miR-34a overexpression could remit the inhibition. CONCLUSION:Results from this study provide evidence that miR-34a may offer a new approach for the treatment of diabetes.

journal_name

Medicine (Baltimore)

journal_title

Medicine

authors

Zhang X,Song S,Luo H

doi

10.1097/MD.0000000000005050

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

e5050

issue

44

eissn

0025-7974

issn

1536-5964

pii

00005792-201611010-00005

journal_volume

95

pub_type

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