Abstract:
:The staining properties of AT-specific dyes Hoechst 33342 and DAPI as revealed by Hoechst 33342/mithramycin and mithramycin/DAPI bivariate human flow karyotype patterns are different for chromosomes rich in heterochromatin. The peak corresponding to chromosome Y of a given cell line is higher on the A/T axis with mithramycin/DAPI staining than with mithramycin/Hoechst. The chromosome 1 was found slightly more fluorescent in mithramycin/DAPI than in mithramycin/Hoechst as judged by the slight displacement of its area on the Hoechst-DAPI axis. The other peaks did not show major differences. On the same flow karyotypes, chromosomal rearrangements were detected.
journal_name
Hum Genetjournal_title
Human geneticsauthors
Bernheim A,Miglierina Rdoi
10.1007/BF00286716subject
Has Abstractpub_date
1989-09-01 00:00:00pages
189-93issue
2eissn
0340-6717issn
1432-1203journal_volume
83pub_type
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