Metaproteomics of saliva identifies human protein markers specific for individuals with periodontitis and dental caries compared to orally healthy controls.

Abstract:

BACKGROUND:The composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. To identify characteristics of diseased and healthy saliva we thus wanted to compare saliva metaproteomes from patients with periodontitis and dental caries to healthy individuals. METHODS:Stimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals. The proteins in the saliva samples were subjected to denaturing buffer and digested enzymatically with LysC and trypsin. The resulting peptide mixtures were cleaned up by solid-phase extraction and separated online with 2 h gradients by nano-scale C18 reversed-phase chromatography connected to a mass spectrometer through an electrospray source. The eluting peptides were analyzed on a tandem mass spectrometer operated in data-dependent acquisition mode. RESULTS:We identified a total of 35,664 unique peptides from 4,161 different proteins, of which 1,946 and 2,090 were of bacterial and human origin, respectively. The human protein profiles displayed significant overexpression of the complement system and inflammatory markers in periodontitis and dental caries compared to healthy controls. Bacterial proteome profiles and functional annotation were very similar in health and disease. CONCLUSIONS:Overexpression of proteins related to the complement system and inflammation seems to correlate with oral disease status. Similar bacterial proteomes in healthy and diseased individuals suggests that the salivary microbiota predominantly thrives in a planktonic state expressing no disease-associated characteristics of metabolic activity.

journal_name

PeerJ

journal_title

PeerJ

authors

Belstrøm D,Jersie-Christensen RR,Lyon D,Damgaard C,Jensen LJ,Holmstrup P,Olsen JV

doi

10.7717/peerj.2433

subject

Has Abstract

pub_date

2016-09-14 00:00:00

pages

e2433

issn

2167-8359

pii

2433

journal_volume

4

pub_type

杂志文章

相关文献

PeerJ文献大全
  • Genetic divergence and fine scale population structure of the common bottlenose dolphin (Tursiops truncatus, Montagu) found in the Gulf of Guayaquil, Ecuador.

    abstract::The common bottlenose dolphin, Tursiops truncatus, is widely distributed along the western coast of South America. In Ecuador, a resident population of bottlenose dolphins inhabits the inner estuarine area of the Gulf of Guayaquil located in the southwestern part of the country and is under threat from different human...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4589

    authors: Bayas-Rea RLÁ,Félix F,Montufar R

    更新日期:2018-04-09 00:00:00

  • Meta-analysis reveals that seed-applied neonicotinoids and pyrethroids have similar negative effects on abundance of arthropod natural enemies.

    abstract:BACKGROUND:Seed-applied neonicotinoids are widely used in agriculture, yet their effects on non-target species remain incompletely understood. One important group of non-target species is arthropod natural enemies (predators and parasitoids), which contribute considerably to suppression of crop pests. We hypothesized t...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2776

    authors: Douglas MR,Tooker JF

    更新日期:2016-12-07 00:00:00

  • The effects of growth rate and biomechanical loading on bone laminarity within the emu skeleton.

    abstract::The orientation of vascular canals in primary bone may reflect differences in growth rate and/or adaptation to biomechanical loads. Previous studies link specific canal orientations to bone growth rates, but results between different taxa are contradictory. Circumferential vascular canals (forming laminar bone) have b...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7616

    authors: Kuehn AL,Lee AH,Main RP,Simons ELR

    更新日期:2019-09-25 00:00:00

  • Worldwide inequality in access to full text scientific articles: the example of ophthalmology.

    abstract:Background:The problem of access to medical information, particularly in low-income countries, has been under discussion for many years. Although a number of developments have occurred in the last decade (e.g., the open access (OA) movement and the website Sci-Hub), everyone agrees that these difficulties still persist...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7850

    authors: Boudry C,Alvarez-Muñoz P,Arencibia-Jorge R,Ayena D,Brouwer NJ,Chaudhuri Z,Chawner B,Epee E,Erraïs K,Fotouhi A,Gharaibeh AM,Hassanein DH,Herwig-Carl MC,Howard K,Kaimbo Wa Kaimbo D,Laughrea PA,Lopez FA,Machin-Mastromatteo

    更新日期:2019-10-30 00:00:00

  • The secret life of deep-sea shrimps: ecological and evolutionary clues from the larval description of Systellaspis debilis (Caridea: Oplophoridae).

    abstract::Currently there are 21 shrimp species in the northeastern Atlantic and Mediterranean Sea which are considered to belong to the superfamily Oplophoroidea, but the larval development is unknown for most of them. The complete larval development of Systellaspis debilis (Milne-Edwards, 1881), here described and illustrated...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7334

    authors: Bartilotti C,Dos Santos A

    更新日期:2019-09-18 00:00:00

  • 15-LOX metabolites and angiogenesis: angiostatic effect of 15(S)-HPETE involves induction of apoptosis in adipose endothelial cells.

    abstract::Inflammation is critical in the dysregulated growth of adipose tissue and associated vascular dysfunctions. 15-Lipoxygenase metabolites, important mediators of inflammation in adipose tissue during obese conditions, may contribute to codependence of inflammation and angiogenesis in adipose tissue. We have already repo...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.635

    authors: Soumya SJ,Binu S,Helen A,Reddanna P,Sudhakaran PR

    更新日期:2014-10-21 00:00:00

  • Toward an integrated approach to crop production and pollination ecology through the application of remote sensing.

    abstract::Insect pollinators provide an essential ecosystem service by transferring pollen to crops and native vegetation. The extent to which pollinator communities vary both spatially and temporally has important implications for ecology, conservation and agricultural production. However, understanding the complex interaction...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5806

    authors: Willcox BK,Robson AJ,Howlett BG,Rader R

    更新日期:2018-10-19 00:00:00

  • No evidence that crayfish carcasses produce detectable environmental DNA (eDNA) in a stream enclosure experiment.

    abstract::Environmental DNA (eDNA) is an emerging tool for monitoring invasive and imperiled species, particularly at low densities. However, the factors that control eDNA production, transport, and persistence in aquatic systems remain poorly understood. For example, the extent to which carcasses produce detectable eDNA is unk...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9333

    authors: Curtis AN,Larson ER

    更新日期:2020-06-11 00:00:00

  • Involvement of cortical midline structures in the processing of autobiographical information.

    abstract::The term autobiographical self has been used to refer to a mental state that permits reflection on self-identity and personality and the answer to related questions (Damasio, 1998). It requires the retrieval and integrated assembly of memories of facts and events that define an individual's biography. The neural mecha...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.481

    authors: Araujo HF,Kaplan J,Damasio H,Damasio A

    更新日期:2014-07-22 00:00:00

  • Threshold-like associations as a function of disturbance.

    abstract::According to the intelligence-creativity threshold hypothesis, there should be a positive association between intelligence and creative potential up to a certain point, the threshold, after which a further increase in intelligence should have no association with creativity. In the present simulation study, the measure...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7891

    authors: Sorjonen K,Ingre M,Melin B

    更新日期:2019-10-21 00:00:00

  • Comparative functional genomic analysis of Alzheimer's affected and naturally aging brains.

    abstract:Background:Alzheimer's disease (AD) is a prevalent progressive neurodegenerative human disease whose cause remains unclear. Numerous initially highly hopeful anti-AD drugs based on the amyloid-β (Aβ) hypothesis of AD have failed recent late-phase tests. Natural aging (AG) is a high-risk factor for AD. Here, we aim to g...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8682

    authors: Peng YS,Tang CW,Peng YY,Chang H,Chen CL,Guo SL,Wu LC,Huang MC,Lee HC

    更新日期:2020-03-20 00:00:00

  • A relationship between species richness and evenness that depends on specific relative abundance distribution.

    abstract::Although many ecologists focus on the relationship between species richness (S) and evenness (E), conflicts between observation and theory are difficult to reconcile. Empirical S-E relationships were not consistent, while relationships show strong correlation between S and E. Since E essentially depended on the relati...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4951

    authors: Su Q

    更新日期:2018-06-12 00:00:00

  • Herbarium-based studies on taxonomy, biogeography and ecology of Psilochilus (Orchidaceae).

    abstract:BACKGROUND:Psilochilus is a poorly studied orchid genus distributed from southern Mexico to south-eastern Brazil. A taxonomic revision of this Neotropical endemic based on morphological data is presented. MATERIAL AND METHODS:Over 170 dried herbarium specimens and flowers preserved in liquid of Psilochilus were analyz...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2600

    authors: Kolanowska M,Naczk AM,Jaskuła R

    更新日期:2016-11-08 00:00:00

  • A six-year grazing exclusion changed plant species diversity of a Stipa breviflora desert steppe community, northern China.

    abstract::Excluding grazers is one of most efficient ways to restore degraded grasslands in desert-steppe communities, but may negatively affect the recovery of plant species diversity. However, diversity differences between grazed and fenced grasslands in desert-steppe are poorly known. In a Stipa breviflora desert steppe comm...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4359

    authors: Wang X,Yang X,Wang L,Chen L,Song N,Gu J,Xue Y

    更新日期:2018-02-13 00:00:00

  • Does the sex difference in competitiveness decrease in selective sub-populations? A test with intercollegiate distance runners.

    abstract::Sex differences in some preferences and motivations are well established, but it is unclear whether they persist in selective sub-populations, such as expert financial decision makers, top scientists, or elite athletes. We addressed this issue by studying competitiveness in 1,147 varsity intercollegiate distance runne...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.884

    authors: Deaner RO,Lowen A,Rogers W,Saksa E

    更新日期:2015-04-21 00:00:00

  • Direct imaging of APP proteolysis in living cells.

    abstract::Alzheimer's disease is a multifactorial disorder caused by the interaction of genetic, epigenetic and environmental factors. The formation of cytotoxic oligomers consisting of Aβ peptide is widely accepted as being one of the main key events triggering the development of Alzheimer's disease. Aβ peptide production resu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3086

    authors: Parenti N,Del Grosso A,Antoni C,Cecchini M,Corradetti R,Pavone FS,Calamai M

    更新日期:2017-04-12 00:00:00

  • Fluctuation and diversity of Hydromedusae (Hydrozoa, Cnidaria) in a highly productive region of the Gulf of Mexico inferred from high frequency plankton sampling.

    abstract::Hydrozoa medusae undergo blooms and seasonal fluctuations; however the drivers of such fluctuations are unknown. To understand how medusa populations fluctuate in response to seasonal factors such as temperature, salinity, dissolved oxygen, and chlorophyll a, and to enhance our taxonomic knowledge of Hydrozoa in Galve...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7848

    authors: Pruski S,Miglietta MP

    更新日期:2019-10-08 00:00:00

  • Sampling designs matching species biology produce accurate and affordable abundance indices.

    abstract::Wildlife biologists often use grid-based designs to sample animals and generate abundance estimates. Although sampling in grids is theoretically sound, in application, the method can be logistically difficult and expensive when sampling elusive species inhabiting extensive areas. These factors make it challenging to s...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.227

    authors: Harris G,Farley S,Russell GJ,Butler MJ,Selinger J

    更新日期:2013-12-17 00:00:00

  • Structure and expression analysis of seven salt-related ERF genes of Populus.

    abstract::Ethylene response factors (ERFs) are plant-specific transcription factors (TFs) that play important roles in plant growth and stress defense and have received a great amount of attention in recent years. In this study, seven ERF genes related to abiotic stress tolerance and response were identified in plants of the Po...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10206

    authors: Huang J,Wang S,Wang X,Fan Y,Han Y

    更新日期:2020-10-20 00:00:00

  • Beetles in bamboo forests: community structure in a heterogeneous landscape of southwestern Amazonia.

    abstract::Amazonian bamboo forests dominated by large woody bamboo plants in the genus Guadua cover approximately 180,000 km2 and represent a key resource for many organisms. In southwestern Amazonia, native bamboo forests differ in structure, biodiversity, and growth dynamics from other forest types in the region. However, wit...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5153

    authors: Jacobs JM,von May R,Kavanaugh DH,Connor EF

    更新日期:2018-07-03 00:00:00

  • Diagnostic model of combined ceRNA and DNA methylation related genes in esophageal carcinoma.

    abstract::Esophageal cancer is a common malignant tumor in the world, and the aim of this study was to screen key genes related to the development of esophageal cancer using a variety of bioinformatics analysis tools and analyze their biological functions. The data of esophageal squamous cell carcinoma from the Gene Expression ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8831

    authors: Guan X,Yao Y,Bao G,Wang Y,Zhang A,Zhong X

    更新日期:2020-03-31 00:00:00

  • The effect of pharmacological inhibition of Serine Proteases on neuronal networks in vitro.

    abstract::Neurons are embedded in an extracellular matrix (ECM), which functions both as a scaffold and as a regulator of neuronal function. The ECM is in turn dynamically altered through the action of serine proteases, which break down its constituents. This pathway has been implicated in the regulation of synaptic plasticity ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6796

    authors: Van De Vijver S,Missault S,Van Soom J,Van Der Veken P,Augustyns K,Joossens J,Dedeurwaerdere S,Giugliano M

    更新日期:2019-04-23 00:00:00

  • An adaptive singular spectrum analysis method for extracting brain rhythms of electroencephalography.

    abstract::Artifacts removal and rhythms extraction from electroencephalography (EEG) signals are important for portable and wearable EEG recording devices. Incorporating a novel grouping rule, we proposed an adaptive singular spectrum analysis (SSA) method for artifacts removal and rhythms extraction. Based on the EEG signal am...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3474

    authors: Hu H,Guo S,Liu R,Wang P

    更新日期:2017-06-28 00:00:00

  • White-handed gibbons discriminate context-specific song compositions.

    abstract::White-handed gibbons produce loud and acoustically complex songs when interacting with their neighbours or when encountering predators. In both contexts, songs are assembled from a small number of units although their composition differs in context-specific ways. Here, we investigated whether wild gibbons could infer ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9477

    authors: Andrieu J,Penny SG,Bouchet H,Malaivijitnond S,Reichard UH,Zuberbühler K

    更新日期:2020-08-03 00:00:00

  • The effect of cigarette smoking habits on the outcome of dental implant treatment.

    abstract::The aim of this study was to analyze the influence of smoking habits and other possibly relevant factors on dental implant survival. The study population included all patients who underwent dental implants between the years 1999 and 2008 at a large military dental clinic and were examined in the periodic medical exami...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.546

    authors: Twito D,Sade P

    更新日期:2014-09-02 00:00:00

  • Recruitment of cognitive control regions during effortful self-control is associated with altered brain activity in control and reward systems in dieters during subsequent exposure to food commercials.

    abstract::Engaging in effortful self-control can sometimes impair people's ability to resist subsequent temptations. Existing research has shown that when chronic dieters' self-regulatory capacity is challenged by prior exertion of effort, they demonstrate disinhibited eating and altered patterns of brain activity when exposed ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6550

    authors: Lopez RB,Courtney AL,Wagner DD

    更新日期:2019-02-28 00:00:00

  • Identification of limiting climatic and geographical variables for the distribution of the tortoise Chelonoidis chilensis (Testudinidae): a baseline for conservation actions.

    abstract::Background. Just as for most other tortoise species, the once common Chaco tortoise, Chelonoidis chilensis (Testudinidae), is under constant threat across it distribution in Argentina, Bolivia and Paraguay. Despite initial qualitative description of the species distribution and further individual reports of new locati...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1298

    authors: Ruete A,Leynaud GC

    更新日期:2015-10-01 00:00:00

  • Boat anchoring contributes substantially to coral reef degradation in the British Virgin Islands.

    abstract::Community decline is often linked to anthropogenic activities. Coral reef declines, for example, have been linked to overfishing and climate change, but impacts of coastal development, pollution, and tourism have received increasing attention. Here, we isolated the impact of one little-studied aspect of recreational a...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7010

    authors: Flynn RL,Forrester GE

    更新日期:2019-05-23 00:00:00

  • Worldwide scientific productions with immunotherapy of sepsis: a bibliometric analysis.

    abstract:Background:Sepsis represents a significant healthcare problem worldwide and causes a high number of deaths every year but remains to be fully understood. During and after sepsis, the host immune response is complex and involves an initial excessive host inflammatory response to infection that is closely related to tiss...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7116

    authors: Wan R,Li L,Xing C,Peng R,Gao L

    更新日期:2019-06-17 00:00:00

  • New records of a lost species and a geographic range expansion for sengis in the Horn of Africa.

    abstract::The Somali Sengi or Somali Elephant-shrew ('Elephantulus' revoilii, Macroscelidea, Mammalia) has been considered a "lost species" and is primarily known from about 39 museum specimens, with no new vouchered occurrence records since the early 1970s. The scientific literature contains no data concerning living Somali Se...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9652

    authors: Heritage S,Rayaleh H,Awaleh DG,Rathbun GB

    更新日期:2020-08-18 00:00:00