Contactin‑associated protein‑like 2 expression in SH‑SY5Y cells is upregulated by a FOXP2 mutant with a shortened poly‑glutamine tract.

Abstract:

:The forkhead box protein P2 (FOXP2) gene encodes an important transcription factor that contains a polyglutamine (poly‑Q) tract and a forkhead DNA binding domain. It has been observed that FOXP2 is associated with speech sound disorder (SSD), and mutations that decrease the length of the poly‑Q tract were identified in the FOXP2 gene of SSD patients. However, the exact role of poly‑Q reduction is not well understood. In the present study, constructs expressing wild‑type and poly‑Q reduction mutants of FOXP2 were generated by polymerase chain reaction (PCR) using lentiviral vectors and transfected into the SH‑SY5Y neuronal cell line. Quantitative reverse transcription (qRT)‑PCR and western blotting indicated that infected cells stably expressed high levels of FOXP2. Using this cell model, the impact of FOXP2 on the expression of contactin‑associated protein‑like 2 (CNTNAP2) were investigated, and CNTNAP2 mRNA expression levels were observed to be significantly higher in cells expressing poly‑Q‑reduced FOXP2. In addition, the expression level of CASPR2, a mammalian homolog of Drosophila Neurexin IV, was increased in cells expressing the FOXP2 mutant. Demonstration of regulation by FOXP2 indicates that CNTNAP2 may also be involved in SSD.

journal_name

Mol Med Rep

authors

Zhao Y,Liu X,Sun H,Wang Y,Yang W,Ma H

doi

10.3892/mmr.2015.4483

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

8162-8

issue

6

eissn

1791-2997

issn

1791-3004

journal_volume

12

pub_type

杂志文章
  • Morphological study of the TK cholangiocarcinoma cell line with three-dimensional cell culture.

    abstract::Cholangiocarcinoma is an intractable carcinoma originating from the bile duct epithelium. To gain an understanding of the cell biology of cholangiocarcinoma, in vitro cell culture is valuable. However, well‑characterized cell lines are limited. In the present study, the morphology of the TK cholangiocarcinoma cell lin...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.1939

    authors: Akiyoshi K,Kamada M,Akiyama N,Suzuki M,Watanabe M,Fujioka K,Ikeda K,Mizuno S,Manome Y

    更新日期:2014-04-01 00:00:00

  • Effects of GnRH agonists on the expression of developmental follicular anti-mullerian hormone in varying follicular stages in cyclic mice in vivo.

    abstract::Gonadotrophin‑releasing hormone (GnRH) agonists (GnRHa) have been widely used to induce a state of downregulation for in vitro fertilization, and its direct effects on the pituitary are well known. However, the effects of GnRHa on the expression of anti‑mullerian hormone (AMH) by follicles in varying stages in vivo re...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3993

    authors: Huang J,Wang X,Li Z,Ma R,Xiao W

    更新日期:2015-09-01 00:00:00

  • microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells.

    abstract::Dengue virus (DENV) causes self‑limiting dengue fever (DF), severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It is generally considered that cytokine storm leads to the increased plasma leakage characteristic of DHF/DSS. In the present study, peripheral blood m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1288

    authors: Qi Y,Li Y,Zhang L,Huang J

    更新日期:2013-03-01 00:00:00

  • proBDNF inhibits the proliferation and migration of OLN‑93 oligodendrocytes.

    abstract::In contrast with mature brain‑derived neurotrophic factor (mBDNF), proBDNF induces cell apoptosis. However, the function of proBDNF in oligodendrocytes remains unclear. In the present study, the OLN‑93 oligodendroglia cell line was utilized as an in vitro model to analyse the functions of proBDNF in oligodendroglia. p...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9407

    authors: Liu S,Guo W,Zhou H,Tang L,Feng S,Zhong JH,Zhou XF

    更新日期:2018-10-01 00:00:00

  • MicroRNA-181 inhibits glioma cell proliferation by targeting cyclin B1.

    abstract::Small non‑coding RNAs from the microRNA family (miRs) are important elements in the posttranscriptional control of gene expression. miRs are known to regulate numerous cellular processes and are of crucial importance during development and in pathological conditions, including tumor initiation and progression. In the ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2423

    authors: Wang F,Sun JY,Zhu YH,Liu NT,Wu YF,Yu F

    更新日期:2014-10-01 00:00:00

  • Rebamipide upregulates mucin secretion of intestinal goblet cells via Akt phosphorylation.

    abstract::Mucin is produced and secreted by epithelial goblet cells and is a key component of the innate immune system, acting as a barrier in the intestinal tract. However, no studies have been conducted investigating the increase in mucin secretion to enhance the intestinal barrier function. The present study investigated whe...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7647

    authors: Yasuda-Onozawa Y,Handa O,Naito Y,Ushiroda C,Suyama Y,Toyokawa Y,Murakami T,Yasuda T,Ueda T,Majima A,Hotta Y,Doi T,Tanaka M,Horii Y,Higashimura Y,Mizushima K,Morita M,Uehara Y,Horie H,Fukui A,Dohi O,Okayama T,Y

    更新日期:2017-12-01 00:00:00

  • TIPE2 governs macrophage polarization via negative regulation of mTORC1.

    abstract::Macrophages can be polarized into the inflammatory M1 lineage or the immunomodulatory M2 lineage, depending on the differential tissue microenvironment signaling, specific pathogens or cytokine stimulation. Tumor necrosis factor α‑induced protein 8‑like protein 2 (TIPE2) has been demonstrated to negatively regulate in...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7991

    authors: Jiang Y,Li Q,Zhang Y,Gao Y,Jiang L,Chen Z

    更新日期:2018-01-01 00:00:00

  • Increased burden of rare deleterious variants of the KCNQ1 gene in patients with large‑vessel ischemic stroke.

    abstract::The impact of rare and damaging variants in genes associated with platelet function in large‑vessel ischemic stroke (LVIS) remains unknown. The aim of this study was to investigate the contribution of some of these variants to the genetic susceptibility to LVIS in Polish patients using a deep re‑sequencing of 54 selec...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9987

    authors: Janicki PK,Eyileten C,Ruiz-Velasco V,Pordzik J,Czlonkowska A,Kurkowska-Jastrzebska I,Sugino S,Imamura Kawasawa Y,Mirowska-Guzel D,Postula M

    更新日期:2019-04-01 00:00:00

  • miR-194-5p serves a suppressive role in human keloid fibroblasts via targeting NR2F2.

    abstract::Keloids are a skin fibrotic disease that cause a number of problems for reconstructive surgeons. MicroRNAs (miRs) are crucial for the development of keloids. The present study aimed to investigate the function of the miR‑194‑5p/nuclear receptor subfamily 2 group F member 2 (NR2F2) interactome in human keloid fibroblas...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11695

    authors: Xu Q,Jiang S

    更新日期:2021-01-01 00:00:00

  • Curcumin suppresses cardiac fibroblasts activities by regulating the proliferation and cell cycle via the inhibition of the p38 MAPK/ERK signaling pathway.

    abstract::Cardiac fibrosis is a deleterious effect of many cardiovascular diseases. Previous studies have shown that curcumin has exhibited protective effects on cardiovascular diseases. The aim of the present study was to evaluate the effects of curcumin on the activity of human cardiac fibroblasts (CFs) and to elucidate the u...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9120

    authors: Fang G,Chen S,Huang Q,Chen L,Liao D

    更新日期:2018-08-01 00:00:00

  • A thirteen‑gene set efficiently predicts the prognosis of glioblastoma.

    abstract::Glioblastoma multiforme (GBM) is the most common type of brain cancer; it usually recurs and patients have a short survival time. The present study aimed to construct a gene expression classifier and to screen key genes associated with GBM prognosis. GSE7696 microarray data set included samples from 10 recurrent GBM t...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9801

    authors: Yang H,Jin L,Sun X

    更新日期:2019-03-01 00:00:00

  • Bioinformatics analysis of mRNA and miRNA microarray to identify the key miRNA‑gene pairs in small‑cell lung cancer.

    abstract::Small‑cell lung cancer (SCLC) is a type of lung cancer with early metastasis, and high recurrence and mortality rates. The molecular mechanism is still unclear and further research is required. The aim of the present study was to examine the pathogenesis and potential molecular markers of SCLC by comparing the differe...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10441

    authors: Mao Y,Xue P,Li L,Xu P,Cai Y,Chu X,Jiang P,Zhu S

    更新日期:2019-09-01 00:00:00

  • Expression of C1q in the serum of patients with non‑severe aplastic anemia, and its association with disease severity.

    abstract::A type of aplastic anemia (AA), non-severe aplastic anemia (NSAA) is defined as AA that does not meet the diagnostic criteria of severe aplastic anemia (SAA). Complement component 1q (C1q) has an important role in the pathogenesis of various autoimmune diseases; however, the role of C1q in the immune pathogenesis of N...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9754

    authors: Ding S,Liu C,Li Y,Liu H,Liu Z,Chen T,Zhang T,Shao Z,Fu R

    更新日期:2019-02-01 00:00:00

  • Role of glycoprotein 78 and cidec in hepatic steatosis.

    abstract::Hepatic glycoprotein (gp78), a membrane-anchored E3 ubiquitin ligase, has been reported to be involved in regulating lipid and energy metabolism in animals, and cell death‑inducing DFFA‑like effector c (cidec) has emerged as an important regulator of metabolism, which has been implicated in the process of fat differen...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6834

    authors: Li J,Liu G,Zhang F,Zhang Z,Xu Y,Li Q

    更新日期:2017-08-01 00:00:00

  • High lymphocyte‑to‑monocyte ratio is associated with low α‑fetoprotein expression in patients with hepatitis B virus‑associated hepatocellular carcinoma.

    abstract::The association of the peripheral lymphocyte‑to‑monocyte ratio (LMR) with α‑fetoprotein (AFP) status in patients with AFP‑positive and AFP‑negative hepatocellular carcinoma (HCC) has not been investigated in detail. The aim of the present study was to examine the association between the LMR and AFP status in these pat...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11387

    authors: Wang H,Xiang Y,Li X,Liu S,Liu L

    更新日期:2020-10-01 00:00:00

  • Neuroprotective effect of tanshinone IIA weakens spastic cerebral palsy through inflammation, p38MAPK and VEGF in neonatal rats.

    abstract::As one of main active ingredients of salvia miltiorrhizae, which is a traditional Chinese medicine, tanshinone IIA is the basis of its pharmacological activities. In the present study, the effect of tanshinone IIA on weakening spastic cerebral palsy (SCP) in neonatal rats was investigated. Radial arm water maze and ho...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8069

    authors: Zhang WL,Cao YA,Xia J,Tian L,Yang L,Peng CS

    更新日期:2018-01-01 00:00:00

  • Resistin aggravates the expression of proinflammatory cytokines in cerulein‑stimulated AR42J pancreatic acinar cells.

    abstract::Resistin, an adipocytokine secreted by fat tissues, has been shown to be associated with increased local and systemic complications in acute pancreatitis (AP). However, the mechanism underlying the effect of resistin in the aggravation of AP remains to be elucidated. The aim of the present study was to investigate the...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.6027

    authors: Jiang CY,Wang W

    更新日期:2017-01-01 00:00:00

  • Epithelial‑mesenchymal transition was identified as a potential marker for breast cancer aggressiveness using reverse transcription‑quantitative polymerase chain reaction.

    abstract::The primary cause of breast cancer‑associated mortality is the formation of distant metastasis. During the metastatic process, single tumor cells dissolve from the primary tumor site and undergo various changes in cell adhesion and motility properties. The tumor cells invade the blood stream and travel to different si...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9091

    authors: Andergassen U,Schlenk K,Jeschke U,Sommer H,Kölbl A

    更新日期:2018-08-01 00:00:00

  • TBX5 loss-of-function mutation contributes to atrial fibrillation and atypical Holt-Oram syndrome.

    abstract::Previous genome-wide association studies have demonstrated that single nucleotide polymorphisms in T‑box (TBX)5 are associated with increased susceptibility to atrial fibrillation (AF), and a recent study has causally linked a TBX5 mutation to atypical Holt-Oram syndrome and paroxysmal AF. However, the prevalence and ...

    journal_title:Molecular medicine reports

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.3892/mmr.2016.5043

    authors: Guo DF,Li RG,Yuan F,Shi HY,Hou XM,Qu XK,Xu YJ,Zhang M,Liu X,Jiang JQ,Yang YQ,Qiu XB

    更新日期:2016-05-01 00:00:00

  • [Corrigendum] Pro‑neurogenic effects of andrographolide on RSC96 Schwann cells in vitro.

    abstract::Following the publication of the above article, an interested reader drew to the authors' attention that a pair of the data panels shown in Fig. 4 contained overlapping data. After having consulted their original data, the authors realized that a total of three panels featured in Figs. 4 and 6 had been erroneously sel...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,已发布勘误

    doi:10.3892/mmr.2020.11278

    authors: Xu F,Wu H,Zhang K,Lv P,Zheng L,Zhao J

    更新日期:2020-09-01 00:00:00

  • Dynamic features of liver fibrogenesis and fibrosis resolution in the absence of matrix metalloproteinase‑9.

    abstract::The two‑edged effect of matrix metalloproteinase‑9 (MMP9) makes it difficult to understand its role in liver fibrogenesis and fibrosis resolution. The present study aimed to investigate the dynamic features of liver fibrogenesis and fibrosis resolution in the absence of MMP9. MMP9‑/‑ mice were used to induce liver fib...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10740

    authors: Wang Q,Liu X,Zhang J,Lu L,Feng M,Wang J

    更新日期:2019-12-01 00:00:00

  • Identification of candidate biomarkers and pathways associated with SCLC by bioinformatics analysis.

    abstract::Small cell lung cancer (SCLC) is one of the highly malignant tumors and a serious threat to human health. The aim of the present study was to explore the underlying molecular mechanisms of SCLC. mRNA microarray datasets GSE6044 and GSE11969 were downloaded from Gene Expression Omnibus database, and the differentially ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9095

    authors: Wen P,Chidanguro T,Shi Z,Gu H,Wang N,Wang T,Li Y,Gao J

    更新日期:2018-08-01 00:00:00

  • Diallyl trisulfide reverses drug resistance and lowers the ratio of CD133+ cells in conjunction with methotrexate in a human osteosarcoma drug-resistant cell subline.

    abstract::P-glycoprotein (P-gp) overexpression and tumor stem cells are thought to be important factors in the cross-resistance of cancer cells. There have been no studies on whether diallyl trisulfide (DATS) can reverse drug resistance in human osteosarcoma cells. In the present study, we demonstrated that DATS down-regulated ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000091

    authors: Li J,Liu W,Zhao K,Zhang Y,Li X,Yang Q,Li Z,Li J

    更新日期:2009-03-01 00:00:00

  • Tumor necrosis factor‑α‑induced protein‑8 like 2 regulates lipopolysaccharide‑induced rat rheumatoid arthritis immune responses and is associated with Rac activation and interferon regulatory factor 3 phosphorylation.

    abstract::The endogenously activated rheumatoid arthritis (RA) synovial fibroblasts (RSFs) are likely to be the key to curing the disease. RSFs express Toll‑like receptors (TLRs) rendering them prone to activation by exogenous and endogenous TLR ligands, resulting in the production of chemokines and cytokines Germline deletion ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7311

    authors: Shi C,Wang Y,Zhuang G,Qi Z,Li Y,Yin P

    更新日期:2017-11-01 00:00:00

  • Circadian gene hCLOCK contributes to progression of colorectal carcinoma and is directly regulated by tumor‑suppressive microRNA‑124.

    abstract::An abundance of studies has demonstrated that disruption of circadian rhythms is one of the factors that may contribute to the initiation and development of human colorectal carcinomas (CRCs). Recently, microRNA‑124 has been demonstrated to suppress tumor growth or metastasis of CRCs. However, the mechanisms of cross‑...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7596

    authors: Yu JZ,Sun N,Bei YB,Li XB,Lu C,Hua LC

    更新日期:2017-09-25 00:00:00

  • Blockade of vascular endothelial growth factor-A/receptor 2 exhibits a protective effect on angiotensin-II stimulated podocytes.

    abstract::Vascular endothelial growth factor (VEGF) and Angiotensin II (Ang-II) are important in glomerulosclerosis, which is one of the main causes of chronic kidney disease. Previous studies have demonstrated that angiotensin type 1 receptor blocker (ARB) can inhibit the synthesis of VEGF mediated by Ang‑II and can effectivel...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3911

    authors: Liu X,Zhang H,Wang Q,Yu K,Wang R,Sun J

    更新日期:2015-09-01 00:00:00

  • Immunogenicity of Cpn0425 and its localization in cells infected with Chlamydophila pneumoniae.

    abstract::The present study aimed to determine the intracellular localization of Cpn0425 in Chlamydophila pneumoniae-infected cells. The recombinant plasmid pGEX-6p/Cpn0425 was transformed into E.coli Bl21 cells to express the fusion protein. Following purification with glutathione S-transfer...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1083

    authors: Liu L,You X,Chen L,Zeng Y,Tang S,Yu M,Wu Y,Xhen X

    更新日期:2012-12-01 00:00:00

  • Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells.

    abstract::Twenty‑three clinical Streptococcus pneumoniae (SP) strains were isolated from blood and sputum specimens from the Second Affiliated Hospital of Wenzhou Medical College in 2009. These strains and the ATCC 49619 standard strain were cultured and suspended in normal saline (at a turbidity of 1.0 McFarland). The producti...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1688

    authors: Zhang J,Hu DK,Wang DG,Liu Y,Liu CB,Yu LH,Qu Y,Luo XH,Yang JH,Yu J,Liu SC,Li XY

    更新日期:2013-11-01 00:00:00

  • Survival of pig-to-rhesus corneal xenografts prolonged by prior donor bone marrow transplantation.

    abstract::The aim of the present study was to explore the survival of pig-rhesus corneal xenografts following donor bone marrow transplantation (BMT). Wuzhishan pigs were used as donors and rhesus monkeys as recipients for corneal xenotransplantation. Twelve rhesus monkeys were divided into t...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1294

    authors: Jie Y,Liu L,Pan Z,Wang L

    更新日期:2013-03-01 00:00:00

  • Dickkopf 2 promotes proliferation and invasion via Wnt signaling in prostate cancer.

    abstract::Wnt/β-catenin signaling dysregulation is involved in tumorigenesis. Furthermore, epigenetic modification of the Dickkopf (DKK) family (DKK1‑4) has been shown to be important in the regulation of Wnt signaling. However, the functions and mechanism of DKK2 in the development and progression of prostate cancer remain unc...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5502

    authors: Xu W,Pang K,Zhou ZG,Chen YF,Mo T,Li M,Liu CB

    更新日期:2016-09-01 00:00:00