Abstract:
:The Mediator complex (Mediator) plays key roles in transcription and functions as the nexus for integration of various transcriptional signals. Previously, we screened for Mediator cyclin-dependent kinase (CDK)-interacting factors and identified three proteins related to chromatin regulation. One of them, SUZ12 is required for both stability and activity of Polycomb Repressive Complex 2 (PRC2). PRC2 primarily suppresses gene expression through histone H3 lysine 27 trimethylation, resulting in stem cell maintenance and differentiation; perturbation of this process leads to oncogenesis. Recent work showed that Mediator contributes to the embryonic stem cell state through DNA loop formation, which is strongly associated with chromatin architecture; however, it remains unclear how Mediator regulates gene expression in cooperation with chromatin regulators (i.e. writers, readers and remodelers). We found that Mediator CDKs interact directly with the PRC2 subunit EZH2, as well as SUZ12. Known PRC2 target genes were deregulated by Mediator CDK knockdown during neuronal differentiation, and both Mediator and PRC2 complexes co-occupied the promoters of developmental genes regulated by retinoic acid. Our results provide a mechanistic link between Mediator and PRC2 during neuronal differentiation.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Fukasawa R,Iida S,Tsutsui T,Hirose Y,Ohkuma Ydoi
10.1093/jb/mvv055subject
Has Abstractpub_date
2015-11-01 00:00:00pages
373-84issue
5eissn
0021-924Xissn
1756-2651pii
mvv055journal_volume
158pub_type
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journal_title:Journal of biochemistry
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135395
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更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1093/jb/93.3.687
更新日期:1983-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124924
更新日期:1995-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135278
更新日期:1985-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvx042
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123645
更新日期:1991-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021202
更新日期:1996-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvp076
更新日期:2009-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134528
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pub_type: 杂志文章
doi:10.1093/jb/93.3.895
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pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131364
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pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130979
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pub_type: 杂志文章
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更新日期:1982-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134813
更新日期:1984-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00