Abstract：
:Surface modification of biodegradable vascular grafts is an important strategy to improve the in situ endothelialization of tissue engineered vascular grafts (TEVGs) and prevent major complications associated with current synthetic grafts. Important strategies for improving endothelialization include increasing endothelial cell mobilization and increased endothelial cell capture through biofunctionalization of TEVGs. The objective of this study was to assess two biofunctionalization strategies for improving endothelialization of biodegradable polyester vascular grafts. These techniques consisted of cross-linking heparin to graft surfaces to immobilize vascular endothelial growth factor (VEGF) or antibodies against CD34 (anti-CD34Ab). To this end, heparin, VEGF, and anti-CD34Ab attachment and quantification assays confirmed the efficacy of the modification strategy. Cell attachment and proliferation on these groups were compared to unmodified grafts in vitro and in vivo. To assess in vivo graft functionality, the grafts were implanted as inferior vena cava interpositional conduits in mice. Modified vascular grafts displayed increased endothelial cell attachment and activity in vivo, according to microscopy techniques, histological results, and eNOS expression. Inner lumen diameter of the modified grafts was also better maintained than controls. Overall, while both functionalized grafts outperformed the unmodified control, grafts modified with anti-CD34Ab appeared to yield the most improved results compared to VEGF-loaded grafts.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Melchiorri AJ,Hibino N,Yi T,Lee YU,Sugiura T,Tara S,Shinoka T,Breuer C,Fisher JPdoi
10.1021/bm501853ssubject
Has Abstractpub_date
2015-02-09 00:00:00pages
437-46issue
2eissn
1525-7797issn
1526-4602journal_volume
16pub_type
杂志文章abstract：:Polymerized high internal phase emulsions (polyHIPEs) have been utilized in the creation of injectable scaffolds that cure in situ to fill irregular bone defects and potentially improve tissue healing. Previously, thermally initiated scaffolds required hours to cure, which diminished the potential for clinical transla...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500754r
更新日期：2014-08-11 00:00:00
abstract：:This study describes the formulation and physicochemical characterization of poly(acrylic acid) (PAA) organogels, designed as bioactive implants for improved treatment of infectious diseases of the oral cavity. Organogels were formulated containing a range of concentrations of PAA (3-10% w/w) and metronidazole (2 or 5...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700597e
更新日期：2008-02-01 00:00:00
abstract：:The objective of this study was to explore the use of reverse thermo-responsive (RTG) polymers for generating implants at their site of performance, following minimally invasive surgical procedures. Aiming at combining syringability and enhanced mechanical properties, a new family of injectable RTG-displaying polymers...
journal_title：Biomacromolecules
pub_type：
doi：10.1021/bm0495250
更新日期：2005-05-01 00:00:00
abstract：:Regenerated silk fibroin (RSF) fibers were obtained by extruding a concentrated aqueous silk fibroin solution into an ammonium sulfate coagulation bath. A custom-made simplified industrial-type wet-spinning device with continuous mechanical postdraw was used. The effect of dope concentration, coagulation bath, extrusi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900840h
更新日期：2010-01-11 00:00:00
abstract：:Stability of four dissimilar basic proteins (chymotrypsinogen A, ribonuclease A, cytochrome c, lysozyme) in the complex with four polyanions (heparin, poly(vinylsulfate), poly(4-styrene-sulfonate), Nafion) has been studied by differential scanning calorimetry. The polyanions were chosen because of their different char...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900480t
更新日期：2009-09-14 00:00:00
abstract：:Poloxamer thermoresponsive gels are widely explored in controlled drug delivery. Nevertheless, these gels possess inadequate mechanical properties, poor bioadhesiveness, and high permeability to water. To overcome these issues, we blended mucoadhesive hyaluronic acid (HA) with poloxamer analogs. This study aimed to in...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1008958
更新日期：2011-01-10 00:00:00
abstract：:To devise a sensitive cellulase assay based on substrates having most of the physical characteristics of native cellulose, 5-(4,6-dichlorotriazinyl)aminofluorescein (DTAF) was used as a grafting agent to prepare suspensions of fluorescent microfibrils from bacterial cellulose. These suspensions were digested by a seri...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm020076i
更新日期：2003-05-01 00:00:00
abstract：:Cell cultivation on the surface of a novel poly(N-isopropylacrylamide) (PNIPA) hydrogel (N-NC gel), consisting of a specific type of organic (PNIPA)/inorganic (clay) network, was studied using three cell types; HepG2 human hepatoma cells, human dermal fibroblasts, and human umbilical vein endothelial cells. For the fi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060549b
更新日期：2006-11-01 00:00:00
abstract：:Understanding the self-assembly mechanism of caged proteins provides clues to develop their potential applications in nanotechnology, such as a nanoscale drug delivery system. The E2 protein from Bacillus stearothermophilus , with a virus-like caged structure, has drawn much attention for its potential application as...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201587q
更新日期：2012-03-12 00:00:00
abstract：:Cross-linked homopolymers and copolymers of 2-hydroxyethyl methacrylate, HEMA, and ethylene glycol methacrylate phosphate, MOEP, have been synthesized, and the diffusion of water into these systems has been investigated. Only polymers with 0-20 mol % MOEP exhibited ideal swelling behavior as extensive fracturing occur...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034477p
更新日期：2004-07-01 00:00:00
abstract：:Collagen is a versatile structural molecule in nature and is used as a building block in many highly organized tissues, such as bone, skin, and cornea. The functionality and performance of these tissues are controlled by their hierarchical organization ranging from the molecular up to macroscopic length scales. In the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201008b
更新日期：2011-11-14 00:00:00
abstract：:Structural properties of different polypeptide multilayer nanofilms fabricated at neutral pH have been analyzed by UV spectroscopy, circular dichroism spectroscopy (CD), and Fourier-transform infrared spectroscopy (FTIR). The various peptides studied exhibit a strong tendency to adopt a beta sheet conformation in the ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700967k
更新日期：2008-01-01 00:00:00
abstract：:This paper introduces a chitosan microneedle patch for efficient and sustained transdermal delivery of hydrophilic macromolecules. Chitosan microneedles have sufficient mechanical strength to be inserted in vitro into porcine skin at approximately 250 μm in depth and in vivo into rat skin at approximately 200 μm in de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301293d
更新日期：2012-12-10 00:00:00
abstract：:We demonstrate the formation of polyethylene glycol (PEG) based hydrogels via oxime ligation and the photoinitiated thiol-ene 3D patterning of peptides within the hydrogel matrix postgelation. The gelation process and final mechanical strength of the hydrogels can be tuned using pH and the catalyst concentration. The ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401133r
更新日期：2013-10-14 00:00:00
abstract：:Poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone) has been synthesized and characterized by gel permeation chromatography, Fourier transform infrared spectroscopy, NMR spectroscopy, and thermal analysis. The polymer is a chemically amplified photoresist. Arrays of lines with 25 microm width and 25 microm spacing wer...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0256505
更新日期：2003-01-01 00:00:00
abstract：:Multifunctional and highly biocompatible polyether structures play a key role in shielding liposomes from degradation in the bloodstream, providing also multiple functional groups for further attachment of targeting moieties. In this work hyperbranched polyglycerol ( hbPG) bearing lipids with long alkyl chain anchor a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00115
更新日期：2018-07-09 00:00:00
abstract：:The dynamics of silk protein in the presence and absence of water has been investigated by dielectric relaxation spectroscopy (DRS). The silk fibroin film cast from its water solution contains 4-7 wt % bound water molecules, which can be removed by dehydration at 165 °C. Temperature and frequency scans were performed ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1008316
更新日期：2010-10-11 00:00:00
abstract：:Inulin, the polydisperse reserve polysaccharide from chicory, has been modified by carbamoylation in organic solvents. The reaction of inulin with a range of alkyl isocyanates resulted, after crystalization, in a variety of carbamoylated inulins from which the interfacial properties were determined. The medium and lon...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015570l
更新日期：2001-01-01 00:00:00
abstract：:Two organosolv lignins from different origins, namely, almond shells and maritime pine, were modified by using a nanoclay and nanosilicate. Prior to modification, they were activated via glyoxalation to enhance the reactivity of the lignins and thus ease the introduction of the nanoparticles into their structure. The ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00799
更新日期：2019-09-09 00:00:00
abstract：:The extracellular matrix is an environment rich with structural, mechanical, and molecular signals that can impact cell biology. Traditional approaches in hydrogel biomaterial design often rely on modifying the concentration of cross-linking groups to adjust mechanical properties. However, this strategy provides limit...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00118
更新日期：2017-04-10 00:00:00
abstract：:Effects of molecular weight (Lc) and field strength (E) on conformation of polymer chains and their density are investigated at the surface and bulk of a driven chain system in three dimensions with a Monte Carlo simulation. As the polymer chains deposit on the surface, a linear density gradient develops in low field....
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm000289c
更新日期：2000-10-01 00:00:00
abstract：:The effect of increasing water composition on the rheological and microstructural behavior of a ternary cellulose acetate (CA)/N,N-dimethylacetamide (DMA)/water system is examined. Addition of water to the CA/DMA system results in enhanced steady shear viscosity and dynamic viscoelastic properties and ultimately to ph...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0611681
更新日期：2007-05-01 00:00:00
abstract：:A novel methacrylate monomer containing a quinolone moiety was synthesized and homopolymerized in N,N-dimethylformamide (DMF) by using azobisisobutyronitrile (AIBN) as an initiator. The new monomer was copolymerized with poly(ethylene glycol) methyl ether methacrylate (MPEGMA) in DMF using the same initiator. The mono...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049383+
更新日期：2005-01-01 00:00:00
abstract：:Subunit vaccines can have excellent safety profiles, but their ability to give rise to robust immune responses is often compromised. For glycan-based vaccines, insufficient understanding of B and T cell epitope combinations that yield optimal immune activation hinders optimization. To determine which antigen features ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01049
更新日期：2019-12-09 00:00:00
abstract：:Sulfate cellulose nanocrystal (CNC) dispersions always present specific self-assembled cholesteric mesophases which is easily affected by the inherent properties of particle size, surface charge, and repulsion or affinity interaction, and external field force generated from ionic potential of added electrolytes, magne...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00927
更新日期：2017-10-09 00:00:00
abstract：:Cellulose nanofibers were prepared by TEMPO-mediated oxidation of wood pulp and tunicate cellulose. The cellulose nanofiber suspension in water was spun into an acetone coagulation bath. The spinning rate was varied from 0.1 to 100 m/min to align the nanofibers to the spun fibers. The fibers spun from the wood nanofib...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm101510r
更新日期：2011-03-14 00:00:00
abstract：:Ionic liquids (ILs) are composed only of ions. Of special interest to this review are those where at least one ion (the cation) is organic and whose melting points are below or not far above room temperature. ILs are designated as "green" solvents because they have extremely low vapor pressure, are non-inflammable, an...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm070062i
更新日期：2007-09-01 00:00:00
abstract：:We describe investigations of insulin release from thermoresponsive microgels using variable temperature (1)H NMR. Microgel particles composed of poly(N-isopropylacrylamide) were loaded with the peptide via a swelling technique, and this method was compared to simple equilibrium partitioning. Variable temperature (1)H...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060718s
更新日期：2006-10-01 00:00:00
abstract：:We report the engineering of intracellular redox-responsive nanoporous poly(ethylene glycol)-poly(l-lysine) particles (NPEG-PLLs). The obtained particles exhibit no toxicity while maintaining the capability to deliver a small interfering RNA sequence (siRNA) targeting the anti-apoptotic factor, survivin, in prostate c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00562
更新日期：2015-07-13 00:00:00
abstract：:This work reports on the effect of CO2 at subcritical conditions and the gaseous state on the telechelic poly(epsilon-caprolactone) polymers. The tested polymers are semi-crystalline in nature and thus the effect of functional groups and their overall contribution to foaming and formation of microstructures with open-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050223n
更新日期：2005-09-01 00:00:00
