Abstract：
:We analysed the global protein expression in seeds of a high-oil soybean cultivar (Jiyu 73, JY73) by proteomics. More than 700 protein spots were detected and 363 protein spots were successfully identified. Comparison of the protein profile of JY73 with that of a high-protein cultivar (Zhonghuang 13, ZH13) revealed 40 differentially expressed proteins, including oil synthesis, redox/stress, hydrolysis and storage-related proteins. All redox/stress proteins were less or not expressed in JY73, whereas the expression of the major storage proteins, nitrogen and carbon metabolism-related proteins was higher in ZH13. Biochemical analysis of JY73 revealed that it was in a low oxidation state, with a high content of polyunsaturated fatty acids and vitamin E. Vitamin E was more active than antioxidant enzymes and protected the soybean seed in a lower oxidation state. The characteristics of high oil and high protein in soybean, we revealed, might provide a reference for soybean nutrition and soybean breeding.
journal_name
Food Chemjournal_title
Food chemistryauthors
Xu XP,Liu H,Tian L,Dong XB,Shen SH,Qu le Qdoi
10.1016/j.foodchem.2014.09.035subject
Has Abstractpub_date
2015-04-01 00:00:00pages
105-16eissn
0308-8146issn
1873-7072pii
S0308-8146(14)01402-2journal_volume
172pub_type
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