Evolution of the miR199-214 cluster and vertebrate skeletal development.


:MicroRNA (miRs) are short non-coding RNAs that fine-tune the regulation of gene expression to coordinate a wide range of biological processes. MicroRNAs are transcribed from miR genes and primary miR transcripts are processed to approximately 22 nucleotide single strand mature forms that function as repressors of transcript translation when bound to the 3'UTR of protein coding transcripts in association with the RISC. Because of their role in the regulation of gene expression, miRs are essential players in development by acting on cell fate determination and progression toward cell differentiation. The miR199 and miR214 genes occupy an intronic cluster located on the opposite strand of the Dynamin3 gene. These miRNAs play major roles in a broad variety of developmental processes and diseases, including skeletal development and several types of cancer. In the work reported here, we first deciphered the origin of the miR199 and miR214 families by following evolution of miR paralogs and their host Dynamin paralogs. We then examined the expression patterns of miR199 and miR214 in developing zebrafish embryos and demonstrated their regulation through a common primary transcript. Results suggest an evolutionarily conserved regulation across vertebrate lineages. Our expression study showed predominant expression patterns for both miR in tissues surrounding developing craniofacial skeletal elements consistent with expression data in mouse and human, thus indicating a conserved role of miR199 and miR214 in vertebrate skeletogenesis.


RNA Biol


RNA biology


Desvignes T,Contreras A,Postlethwait JH




Has Abstract


2014-01-01 00:00:00














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    abstract::MicroRNAs are small RNAs that post-transcriptionally regulate eukaryotic gene expression. In addition to their involvement in a wide range of physiological and pathological processes, including viral infections, microRNAs are increasingly implicated in the eukaryotic response to bacterial pathogens. Recent studies hav...

    journal_title:RNA biology

    pub_type: 杂志文章,评审


    authors: Eulalio A,Schulte L,Vogel J

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  • Variable splicing of non-coding roX2 RNAs influences targeting of MSL dosage compensation complexes in Drosophila.

    abstract::The non-coding roX1 and roX2 RNAs are components of the MSL dosage compensation complex in Drosophila. We found that multiple species of roX2 RNA are produced by alternative splicing, with one major and at least 20 different minor forms associated with MSL proteins. The alternative forms are generated by variable usag...

    journal_title:RNA biology

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    authors: Park Y,Oh H,Meller VH,Kuroda MI

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    abstract::I am honored to have been asked to contribute to this memorial issue, although I cannot claim to have known Carl Woese well. Carl's insights and the discoveries that his research group made over the years certainly stimulated my own research program, and at several points early on, interactions with him were pivotal i...

    journal_title:RNA biology

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    authors: Gray MW

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  • High-affinity interaction of hnRNP A1 with conserved RNA structural elements is required for translation and replication of enterovirus 71.

    abstract::Human Enterovirus 71 (EV71) is an emerging pathogen of infectious disease and a serious threat to public health. Currently, there are no antivirals or vaccines to slow down or prevent EV71 infections, thus underscoring the urgency to better understand mechanisms of host-enterovirus interactions. EV71 uses a type I int...

    journal_title:RNA biology

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    authors: Levengood JD,Tolbert M,Li ML,Tolbert BS

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  • Identification of a region of hantavirus nucleocapsid protein required for RNA chaperone activity.

    abstract::Sin Nombre hantavirus (SNV) is a New World hantavirus and causes hantavirus cardiopulmonary syndrome. The viral nucleocapsid protein (N) is an RNA chaperone and has multiple functions important in virus replication. The three negative sense RNA segments of hantaviruses form panhandle structures through imperfect hydro...

    journal_title:RNA biology

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    authors: Brown BA,Panganiban AT

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  • Genome-wide technology for determining RNA stability in mammalian cells: historical perspective and recent advantages based on modified nucleotide labeling.

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    journal_title:RNA biology

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    authors: Tani H,Akimitsu N

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  • Variations on the tmRNA gene.

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    authors: Mao C,Bhardwaj K,Sharkady SM,Fish RI,Driscoll T,Wower J,Zwieb C,Sobral BW,Williams KP

    更新日期:2009-09-01 00:00:00

  • Screening of small molecules affecting mammalian P-body assembly uncovers links with diverse intracellular processes and organelle physiology.

    abstract::Processing bodies (P-bodies) are cytoplasmatic mRNP granules containing non-translating mRNAs and proteins from the mRNA decay and silencing machineries. The mechanism of P-body assembly has been typically addressed by depleting P-body components. Here we apply a complementary approach and establish an automated cell-...

    journal_title:RNA biology

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    更新日期:2013-11-01 00:00:00

  • Characterization of cis-acting elements that control oscillating alternative splicing.

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    authors: Goldammer G,Neumann A,Strauch M,Müller-McNicoll M,Heyd F,Preußner M

    更新日期:2018-01-01 00:00:00

  • Wobble uridine modifications-a reason to live, a reason to die?!

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    journal_title:RNA biology

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    authors: Domitrovic T,Moreira MH,Carneiro RL,Ribeiro-Alves M,Palhano FL

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    pub_type: 杂志文章


    authors: Resch A,Većerek B,Palavra K,Bläsi U

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  • Processive RNA decay by the exosome: merits of a quantitative Bayesian sampling approach.

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    authors: Niederberger T,Hartung S,Hopfner KP,Tresch A

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  • FMR1 RNA within the intranuclear inclusions of fragile X-associated tremor/ataxia syndrome (FXTAS).

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    authors: Niemelä EH,Frilander MJ

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  • RNA remodeling and gene regulation by cold shock proteins.

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    journal_title:RNA biology

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    authors: Phadtare S,Severinov K

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  • Cleavage factor Im is a key regulator of 3' UTR length.

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    journal_title:RNA biology

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    authors: Gruber AR,Martin G,Keller W,Zavolan M

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    journal_title:RNA biology

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    authors: Richter AS,Backofen R

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  • Insights into lncRNAs in Alzheimer's disease mechanisms.

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    journal_title:RNA biology

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    journal_title:RNA biology

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  • Role and mechanism of U1-independent pre-mRNA splicing in the regulation of alternative splicing.

    abstract::In metazoan organisms, alternative splicing is a central mechanism for the regulation of gene expression. However, many questions remain about the underlying molecular mechanisms. Our recent work suggests that U1 snRNP-independent premRNA splicing occurs in humans, which contributes to the regulation of alternative sp...

    journal_title:RNA biology

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    authors: Fukumura K,Inoue K

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    journal_title:RNA biology

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    authors: Zeng C,Sang Y,Wang FY,Zhuang SM

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  • Toward a crystal-clear view of the viral RNA sensing and response by RIG-I-like receptors.

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    journal_title:RNA biology

    pub_type: 杂志文章,评审


    authors: Luo D

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  • Modeling SNP mediated differential targeting of homologous 3'UTR by microRNA.

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    journal_title:RNA biology

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    authors: Ahluwalia JK,Soni K,Sivasubbu S,Brahmachari V

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  • A novel antisense RNA ASPACT confers multi-level suppression of PACT and associated signalling.

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    journal_title:RNA biology

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    authors: Kuo YP,Ma CP,Chen HW,Chen YT,Lai YH,Liu H,Kuo RL,Chin-Ming Tan B

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  • MicroRNA-432 targeting E2F3 and P55PIK inhibits myogenesis through PI3K/AKT/mTOR signaling pathway.

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    journal_title:RNA biology

    pub_type: 杂志文章


    authors: Ma M,Wang X,Chen X,Cai R,Chen F,Dong W,Yang G,Pang W

    更新日期:2017-03-04 00:00:00

  • Bi-phased regulation of the post-transcriptional inflammatory response by Tristetraprolin levels.

    abstract::AU-rich elements (AREs) are cis-acting instability and translation inhibition elements that are present in the 3'UTR of most inducible inflammatory mRNAs such as TNF and Cxcl2. mRNAs that contain AREs are, by default, repressed and only transiently expressed in response to stimuli. They are targeted by the inducible R...

    journal_title:RNA biology

    pub_type: 杂志文章


    authors: Mahmoud L,Moghrabi W,Khabar KSA,Hitti EG

    更新日期:2019-03-01 00:00:00