Abstract:
:Bisulfite sequencing (BS-seq) is the gold standard for studying genome-wide DNA methylation. We developed MOABS to increase the speed, accuracy, statistical power and biological relevance of BS-seq data analysis. MOABS detects differential methylation with 10-fold coverage at single-CpG resolution based on a Beta-Binomial hierarchical model and is capable of processing two billion reads in 24 CPU hours. Here, using simulated and real BS-seq data, we demonstrate that MOABS outperforms other leading algorithms, such as Fisher's exact test and BSmooth. Furthermore, MOABS analysis can be easily extended to differential 5hmC analysis using RRBS and oxBS-seq. MOABS is available at http://code.google.com/p/moabs/.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Sun D,Xi Y,Rodriguez B,Park HJ,Tong P,Meong M,Goodell MA,Li Wdoi
10.1186/gb-2014-15-2-r38subject
Has Abstractpub_date
2014-02-24 00:00:00pages
R38issue
2eissn
1474-7596issn
1474-760Xpii
gb-2014-15-2-r38journal_volume
15pub_type
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