Abstract:
:Biogenesis of mammalian mitochondrial ribosomes requires a concerted maturation of both the small (SSU) and large subunit (LSU). We demonstrate here that the m(5)C methyltransferase NSUN4, which forms a complex with MTERF4, is essential in mitochondrial ribosomal biogenesis as mitochondrial translation is abolished in conditional Nsun4 mouse knockouts. Deep sequencing of bisulfite-treated RNA shows that NSUN4 methylates cytosine 911 in 12S rRNA (m5C911) of the SSU. Surprisingly, NSUN4 does not need MTERF4 to generate this modification. Instead, the NSUN4/MTERF4 complex is required to assemble the SSU and LSU to form a monosome. NSUN4 is thus a dual function protein, which on the one hand is needed for 12S rRNA methylation and, on the other hand interacts with MTERF4 to facilitate monosome assembly. The presented data suggest that NSUN4 has a key role in controlling a final step in ribosome biogenesis to ensure that only the mature SSU and LSU are assembled.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Metodiev MD,Spåhr H,Loguercio Polosa P,Meharg C,Becker C,Altmueller J,Habermann B,Larsson NG,Ruzzenente Bdoi
10.1371/journal.pgen.1004110subject
Has Abstractpub_date
2014-02-06 00:00:00pages
e1004110issue
2eissn
1553-7390issn
1553-7404pii
PGENETICS-D-13-01656journal_volume
10pub_type
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