Abstract:
:H1 linker histones facilitate higher-order chromatin folding and are essential for mammalian development. To achieve high-resolution mapping of H1 variants H1d and H1c in embryonic stem cells (ESCs), we have established a knock-in system and shown that the N-terminally tagged H1 proteins are functionally interchangeable to their endogenous counterparts in vivo. H1d and H1c are depleted from GC- and gene-rich regions and active promoters, inversely correlated with H3K4me3, but positively correlated with H3K9me3 and associated with characteristic sequence features. Surprisingly, both H1d and H1c are significantly enriched at major satellites, which display increased nucleosome spacing compared with bulk chromatin. While also depleted at active promoters and enriched at major satellites, overexpressed H1(0) displays differential binding patterns in specific repetitive sequences compared with H1d and H1c. Depletion of H1c, H1d, and H1e causes pericentric chromocenter clustering and de-repression of major satellites. These results integrate the localization of an understudied type of chromatin proteins, namely the H1 variants, into the epigenome map of mouse ESCs, and we identify significant changes at pericentric heterochromatin upon depletion of this epigenetic mark.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Cao K,Lailler N,Zhang Y,Kumar A,Uppal K,Liu Z,Lee EK,Wu H,Medrzycki M,Pan C,Ho PY,Cooper GP Jr,Dong X,Bock C,Bouhassira EE,Fan Ydoi
10.1371/journal.pgen.1003417subject
Has Abstractpub_date
2013-01-01 00:00:00pages
e1003417issue
4eissn
1553-7390issn
1553-7404pii
PGENETICS-D-12-00919journal_volume
9pub_type
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