Lethal hypothermia in an animal model, not associated with basal renal epithelial vacuolization.

Abstract:

:A rodent model was used to evaluate the association between hypothermia and basal vacuolization in renal tubular epithelial cells. 28 Sprague Dawley rats were anaesthetized in non-stressful conditions and placed two at a time into a cooling chamber. Body core temperatures dropped to a minimum of 7-10 °C, causing death under anaesthesia at times varying from 120 to 240 min. The animals were then subjected to necropsy; the kidneys were removed and placed in 10% buffered formalin. Examination of haematoxylin and eosin-stained renal sections failed to reveal basal vacuolization of renal tubular epithelial cells in any of the 28 animals. In this model, no evidence of subnuclear lipid vacuolization of renal tubular cells could be demonstrated despite significant and eventually lethal hypothermia. These results lend support to the hypothesis that the basal vacuolization in hypothermia may be a manifestation of a more complex pathophysiological pathway rather than being due simply to low body core temperatures.

journal_name

J Forensic Leg Med

authors

Zhou C,Bright F,Winskog C,Yool AJ,Byard RW

doi

10.1016/j.jflm.2013.10.014

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

14-6

eissn

1752-928X

issn

1878-7487

pii

S1752-928X(13)00288-6

journal_volume

21

pub_type

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