Abstract:
BACKGROUND AND AIMS:The ω-gliadin storage proteins of wheat are of interest in relation to their impact on grain processing properties and their role in food allergy, particularly the ω-5 sub-group and wheat-dependent exercise-induced anaphylaxis. The ω-gliadins are also known to be responsive to nitrogen application. This study therefore compares the effects of cultivar and nitrogen availability on the synthesis and deposition of ω-gliadins in wheat grown under field conditions in the UK, including temporal and spatial analyses at the protein and transcript levels. METHODS:SDS-PAGE, western blotting and N-terminal amino acid sequencing were used to compare the patterns of ω-gliadin components in mature grain of six British wheat (Triticum aestivum) cultivars and their accumulation during the development of grain grown in field plots with varying nitrogen supply. Changes in gene expression during development were determined using real-time reverse transcription-PCR (RT-PCR). Spatial patterns of gene expression and protein accumulation were determined by in situ hybridization and immunofluorescence microscopy, respectively. KEY RESULTS:Two patterns of ω-gliadins were identified in the six cultivars, including both monomeric 'gliadin' proteins and subunits present in polymeric 'glutenin' fractions. Increasing the level of nitrogen fertilizer in field plots resulted in increased expression of ω-gliadin transcripts and increased proportions of ω-5 gliadins. Nitrogen supply also affected the spatial patterns of ω-gliadin synthesis and deposition, which were differentially increased in the outer layers of the starchy endosperm with high levels of nitrogen. CONCLUSIONS:Wheat ω-gliadins vary in amount and composition between cultivars, and in their response to nitrogen supply. Their spatial distribution is also affected by nitrogen supply, being most highly concentrated in the sub-aleurone cells of the starchy endosperm under higher nitrogen availability.
journal_name
Ann Botjournal_title
Annals of botanyauthors
Wan Y,Gritsch CS,Hawkesford MJ,Shewry PRdoi
10.1093/aob/mct291subject
Has Abstractpub_date
2014-03-01 00:00:00pages
607-15issue
4eissn
0305-7364issn
1095-8290pii
mct291journal_volume
113pub_type
杂志文章相关文献
ANNALS OF BOTANY文献大全abstract:BACKGROUND AND AIMS:Numerous studies have examined the effects of climatic factors on the distribution of C(3) and C(4) grasses in various regions throughout the world, but the role of seasonal fluctuations in temperature, precipitation and soil N availability in regulating growth and competition of these two functiona...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcm178
更新日期:2007-10-01 00:00:00
abstract::Columella cells of seedlings of Zea mays L. cv. Bear Hybrid grown in the microgravity of orbital flight allocate significantly larger relative-volumes to hyaloplasm and lipid bodies, and significantly smaller relative-volumes to dictyosomes, plastids, and starch than do columella cells of seedlings grown at 1 g. The...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/oxfordjournals.aob.a087926
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND AIMS:The interspecific allometry of maximum plant height (Hmax) with respect to maximum basal stem diameter (Dmax) has been studied for leptocaulis dicot and conifer tree species. In contrast, virtually nothing is known about the interspecific allometry of pachycaulis species. Here, the interspecific all...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcj002
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND AND AIMS:It is an enduring question as to the mechanisms leading to the high diversity and the processes producing endemics with unusual morphologies in the Himalayan alpine region. In the present study, the phylogenetic relationships and origins of three such endemic genera were analysed, Dolomiaea, Diplazo...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcl259
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND:Robustness to genetic and environmental perturbation is a salient feature of multicellular organisms. Loss of developmental robustness can lead to severe phenotypic defects and fitness loss. However, perfect robustness, i.e. no variation at all, is evolutionarily unfit as organisms must be able to change phe...
journal_title:Annals of botany
pub_type: 杂志文章,评审
doi:10.1093/aob/mcv151
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND AND AIMS:The sedge genus Carex, the most diversified angiosperm genus of the northern temperate zone, is renowned for its holocentric chromosomes and karyotype variability. The genus exhibits high variation in chromosome numbers both among and within species. Despite the possibility that this chromosome evol...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mct119
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Adventitious root (AR) formation in Petunia hybrida is inhibited by low nitrogen fertilization of stock plants but promoted by dark incubation of cuttings before planting. We investigated whether the plant hormone auxin is involved in nitrogen- and dark-mediated AR formation. METHODS:Concentrations...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcz095
更新日期:2019-11-27 00:00:00
abstract:BACKGROUND AND AIMS:Species of the Nepenthaceae family are under-represented in studies of leaf traits and the consequent view of mineral nutrition and limitation in carnivorous plants. This study is aimed to complement existing data on leaf traits of carnivorous plants. METHODS:Physico-chemical properties, including ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcm023
更新日期:2007-05-01 00:00:00
abstract:Background and Aims:Enzymes belonging to the RNase T2 family are essential for normal rRNA turnover in eukaryotes. In Arabidopsis thaliana, this function is performed by RNS2. The null mutant rns2-2 has increased rRNA half-life and constitutive autophagy. The aim of this work was to determine the molecular changes that...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcx099
更新日期:2017-11-28 00:00:00
abstract:BACKGROUND AND AIMS:Differences in the mating systems and the mechanisms of reproductive isolation between Chamaecrista desvauxii var. graminea and C. desvauxii var. latistipula were examined in the Chapada Diamantina, Brazil. These taxa occur sympatrically, and their populations demonstrate marked morphological differ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcm012
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND AND AIMS:Correlated relationships between branch thickness, branch density, and twig and leaf size have been used extensively to study the evolution of plant canopy architecture, but fewer studies have explored the impact of these relationships on the allocation of reproductive resources. This study quantifi...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcs150
更新日期:2012-09-01 00:00:00
abstract:BACKGROUND AND AIMS:Spring geophytes require a period of low temperature for proper flower development but the mechanism that underlies the relationship between cold treatment and flowering remains unknown. The present study aims to compare the developmental anatomy and carbohydrate content of the tuberous geophyte Cor...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcq076
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND:Mineral nutrient limitation affects the water flow through plants. We wanted to test on barley whether any change in root-to-shoot ratio in response to low supply of nitrogen and phosphate is accompanied by changes in root and cell hydraulic properties and involves changes in aquaporin (AQP) gene expression ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcz113
更新日期:2019-11-27 00:00:00
abstract:BACKGROUND:Plants are sessile organisms that have the ability to integrate external cues into metabolic and developmental signals. The cues initiate specific signal cascades that can enhance the tolerance of plants to stress, and these mechanisms are crucial to the survival and fitness of plants. The adaption of plants...
journal_title:Annals of botany
pub_type: 杂志文章,评审
doi:10.1093/aob/mcv063
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND AND AIMS:Plant cyanogenesis is the release of toxic cyanide from endogenous cyanide-containing compounds, typically cyanogenic glycosides. Despite a large body of phytochemical, taxonomic and ecological work on cyanogenic species, little is known of their frequency in natural plant communities. This study ai...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcl048
更新日期:2006-06-01 00:00:00
abstract::Trimenia moorei (Oliv.) Philipson is an andromonoecious liane with >0.40 of the total flower buds maturing as bisexual flowers. Male and bisexual flowers are strongly scented with pollen, anther sacs and receptacle scars testing positively for volatile emissions. Scent analyses detect over 20 components. The major fat...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcg157
更新日期:2003-09-01 00:00:00
abstract:Backgrounds and Aims:Gain or loss of floral nectar, an innovation in floral traits, has occurred in diverse lineages of flowering plants, but the causes of reverse transitions (gain of nectar) remain unclear. Phylogenetic studies show multiple gains and losses of floral nectar in the species-rich genus Pedicularis. Her...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcy102
更新日期:2019-01-23 00:00:00
abstract:BACKGROUND AND AIMS:Cucumber (Cucumis sativus) seedlings develop a peg on the concave side of the gravitropically bending transition zone between the hypocotyl and the root after seed germination. Peg initiation occurs in response to auxin when its levels in the concave side of the transition zone exceed a particular t...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mci045
更新日期:2005-02-01 00:00:00
abstract:AIMS:The objective of the study was to characterize variations in proline, arginine, histidine, vegetative storage proteins, and cold-inducible gene expression in overwintering roots of field-grown alfalfa, in response to autumn defoliation, and in relation to spring regrowth and winter survival. METHODS:Field trials,...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcj006
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Many experimental studies assume that some topological units are autonomous with regard to carbon because it is convenient. Some plant models simulate carbon allocation, employing complex approaches that require calibration and fitted parameters. For whole-tree canopy simulations, simpler carbon all...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcaa098
更新日期:2020-09-14 00:00:00
abstract:BACKGROUND AND AIMS:Despite the importance of vessels in angiosperm roots for plant water transport, there is little research on the microanatomy of woody plant roots. Vessels in roots can be interconnected networks or nearly solitary, with few vessel-vessel connections. Species with few connections are common in arid ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mct294
更新日期:2014-03-01 00:00:00
abstract::Musa L. (Musaceae) is currently separated into five sections (Musa. Rhodochlamys, Callimusa, Australimusa and Ingentimusa) based on chromosome numbers and morphological characters. However, the validation of this classification system is questioned due to the common occurrence of hybridizations across sections and the...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcf170
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Serpentine soils are characterized by the presence of heavy metals (Ni and Cr) and excess Mg; these elements often suppress plant growth. Picea glehnii is nevertheless distributed widely on serpentine soils in northern Japan. Growth characteristics were compared among P. glehnii, Picea jezoensis (di...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mci063
更新日期:2005-03-01 00:00:00
abstract::The objective of this study was to evaluate the role of enzyme induction and aerenchyma formation in prolonged tolerance to soil flooding in a variety of underground clover (Trifolium subterraneum 'Park') previously selected for resistance. Seedlings were grown in hydroponic tanks, initially with aeration for 3 weeks ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcf022
更新日期:2003-01-01 00:00:00
abstract::Background and Aims Allozyme and reproductive data sets for the Canarian flora are updated in order to assess how the present levels and structuring of genetic variation have been influenced by the abiotic island traits and by phylogenetically determined biotic traits of the corresponding taxa; and in order to suggest...
journal_title:Annals of botany
pub_type: 杂志文章,评审
doi:10.1093/aob/mct076
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND AND AIMS:Exogenous ethylene has recently gained commercial interest as a sprouting inhibitor of onion bulbs. The role of ethylene in dormancy and sprouting of onions, however, is not known. METHODS:A cultivar (Allium cepa 'Copra') with a true period of dormancy was used. Dormant and sprouting states of onio...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcn203
更新日期:2009-01-01 00:00:00
abstract::Roots of Allium cepa L. grown in aerated water elongate rapidly, but are not graviresponsive. These roots (1) possess extensive columella tissues comprised of cells containing numerous sedimented amyloplasts, (2) lack mucilage on their tips, and (3) are characterized by a weakly polar movement of calcium (Ca) across ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/oxfordjournals.aob.a087216
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND AND AIMS:During sexual reproduction in higher angiosperms, the pollen tubes are directed to the ovules in the pistil to deliver sperm cells. This pollen tube attraction is highly species specific, and a group of small secreted proteins, TfCRPs, are necessary for this process in Torenia fournieri. METHODS:A ...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcr111
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND AND AIMS:Pollination is a critical stage in plant reproduction and thus in the maintenance and evolution of species and communities. The Caatinga is the fourth largest ecosystem in Brazil, but despite its great extent and its importance few studies providing ecological information are available, with a notab...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mch152
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND AND AIMS:Pea (Pisum sativum) primary roots form long vascular cavities when grown under wet or flooded conditions at 25 degrees C. It is thought that the cavities are a form of aerenchyma. At 25 degrees C short roots continue to grow after flooding. After roots reach 10 cm long flooding causes rapid cessatio...
journal_title:Annals of botany
pub_type: 杂志文章
doi:10.1093/aob/mcl040
更新日期:2006-05-01 00:00:00