Identification and characterization of TcCRP1, a pollen tube attractant from Torenia concolor.

Abstract:

BACKGROUND AND AIMS:During sexual reproduction in higher angiosperms, the pollen tubes are directed to the ovules in the pistil to deliver sperm cells. This pollen tube attraction is highly species specific, and a group of small secreted proteins, TfCRPs, are necessary for this process in Torenia fournieri. METHODS:A candidate pollen tube attractant protein in Torenia concolor, a related species of T. fournieri, was isolated and the attractant abilities between them were compared. KEY RESULTS:TcCRP1, an orthologous gene of TfCRP1 from T. concolor, is expressed predominantly in the synergid cell. The gene product attracted pollen tubes in a concentration-dependent manner, but attracted fewer pollen tubes from the other species. CONCLUSIONS:The results indicated that this class of CRP proteins is a common pollen tube attractant in Torenia species. The sequence diversity of these proteins is important for species-specific pollen tube attraction.

journal_name

Ann Bot

journal_title

Annals of botany

authors

Kanaoka MM,Kawano N,Matsubara Y,Susaki D,Okuda S,Sasaki N,Higashiyama T

doi

10.1093/aob/mcr111

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

739-47

issue

4

eissn

0305-7364

issn

1095-8290

pii

mcr111

journal_volume

108

pub_type

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