Chondrocyte-specific inhibition of β-catenin signaling leads to dysplasia of the caudal vertebrae in mice.

Abstract:

STUDY DESIGN:To inhibit β-catenin specifically signaling in chondrocytes Col2-ICAT transgenic mice were generated. Anomalies in caudal vertebrae were detected during embryonic and postnatal stages of Col2-ICAT transgenic mice. OBJECTIVE:To determine the role of canonical β-catenin signaling in caudal vertebral development. SUMMARY OF BACKGROUND DATA:β-catenin signaling plays a critical role in skeletal development. Col2-ICAT transgenic mice were generated to selectively block β-catenin signaling by overexpression of the ICAT gene in chondrocytes. METHODS:Tails of E16.5 transgenic embryos and adult Col2-ICAT transgenic mice and their wild-type littermates were collected and analyzed. Skeletal preparation, 3-dimensional micro-computed tomographic and histological analyses were performed to evaluate changes in the structure of caudal vertebrae. Bromodeoxyuridine labeling was performed to evaluate changes in chondrocyte proliferation in caudal vertebrae. RESULTS:Skeletal preparation and 3-dimensional micro-computed tomographic analyses revealed bone deformation and angulated deformities in tail tissue in Col2-ICAT transgenic mice. Histological studies revealed abnormal bone development and dysplastic caudal vertebrae in Col2-ICAT transgenic mice. Inhibition of β-catenin signaling in cartilage resulted in vertebral dysplasia leading to aberrant resegmenting process. Thus, 2 poorly developed sclerotomes failed to fuse to form a complete vertebrae. BrdU labeling revealed a decreased chondrocyte proliferation in both cartilageous templates of transgenic embryos and the growth plate of adult Col2-ICAT transgenic mice. CONCLUSION:Wnt/β-catenin signaling plays an important role in vertebral development. Inhibition of β-catenin signaling in chondrocytes results in caudal vertebra deformity in mice, which may occur as early as in the stage of sclerotome formation. LEVEL OF EVIDENCE:N/A.

journal_name

Spine (Phila Pa 1976)

journal_title

Spine

authors

Shu B,Li TF,Li XF,Tang DZ,Zhang Y,Shi Q,Wang YJ,Chen D

doi

10.1097/01.brs.0000435024.57940.8d

subject

Has Abstract

pub_date

2013-11-15 00:00:00

pages

2079-84

issue

24

eissn

0362-2436

issn

1528-1159

journal_volume

38

pub_type

杂志文章

相关文献

SPINE文献大全
  • An Insight Into the Health-Related Quality of Life of Adolescent Idiopathic Scoliosis Patients Who Are Braced, Observed, and Previously Braced.

    abstract:STUDY DESIGN:Prospective cross-sectional study. OBJECTIVE:To determine the health-related quality of life (HRQoL) of adolescent idiopathic scoliosis (AIS) patients undergoing bracing, previously braced and observation only. SUMMARY OF BACKGROUND DATA:HRQoL is an important treatment outcome measure for AIS. A poor HRQ...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000002918

    authors: Cheung PWH,Wong CKH,Cheung JPY

    更新日期:2019-05-15 00:00:00

  • Prevalence of Ossification of Posterior Longitudinal Ligament in Patients With Degenerative Cervical Myelopathy: Cervical Spine 3D CT Observations in 7210 Cases.

    abstract:STUDY DESIGN:A cross-sectional study. OBJECTIVE:The aim of this study was to investigate the prevalence of ossification of posterior longitudinal ligament (OPLL) in patients with degenerative cervical myelopathy (DCM). SUMMARY OF BACKGROUND DATA:OPLL of the cervical spine is one of the main entities of DCM in Asian p...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000003526

    authors: Liao X,Jin Z,Shi L,Zhao Y,Zhou S,Chen D,Tang D,Yang L,Chen X

    更新日期:2020-10-01 00:00:00

  • The effects of an interspinous implant on the kinematics of the instrumented and adjacent levels in the lumbar spine.

    abstract:STUDY DESIGN:Measurement of the kinematics of the lumbar spine after insertion of an interspinous spacer in vitro. OBJECTIVES:To understand the kinematics of the instrumented and adjacent levels due to the insertion of this interspinous implant. SUMMARY OF BACKGROUND DATA:An interspinous spacer (X Stop, SFMT, Concord...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.BRS.0000084877.88192.8E

    authors: Lindsey DP,Swanson KE,Fuchs P,Hsu KY,Zucherman JF,Yerby SA

    更新日期:2003-10-01 00:00:00

  • Postoperative surgical site infections in patients undergoing spinal tumor surgery: incidence and risk factors.

    abstract:STUDY DESIGN:We conducted a retrospective, case control study on patients undergoing surgery for spinal tumors. OBJECTIVE.: Our aim was to determine the incidence and to identify risk factors for surgical site infections (SSIs) in patients undergoing surgery for spinal tumors. SUMMARY OF BACKGROUND DATA:SSIs after spi...

    journal_title:Spine

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/BRS.0b013e3181f48fa9

    authors: Omeis IA,Dhir M,Sciubba DM,Gottfried ON,McGirt MJ,Attenello FJ,Wolinsky JP,Gokaslan ZL

    更新日期:2011-08-01 00:00:00

  • Mechanism of Action of Spinal Mobilizations: A Systematic Review.

    abstract:STUDY DESIGN:Systematic review. OBJECTIVE:To review the evidence regarding the mechanism of action of mobilizations. SUMMARY OF BACKGROUND DATA:Spinal mobilizations-low velocity passive oscillatory movements-reduce spinal pain in some patient subgroups. Identifying patients likely to respond remains a challenge since...

    journal_title:Spine

    pub_type: 杂志文章,评审

    doi:10.1097/BRS.0000000000001151

    authors: Lascurain-Aguirrebeña I,Newham D,Critchley DJ

    更新日期:2016-01-01 00:00:00

  • Trabecular architecture of lumbar vertebral pedicle.

    abstract:STUDY DESIGN:Investigation on architecture of lumbar pedicle. OBJECTIVE:To determine morphological properties of pedicular cancellous bone. SUMMARY OF BACKGROUND DATA:Many researchers have been stimulated to study trabecular architecture by improvements in stereological technology. Although the structure of vertebral...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000168373.24644.9f

    authors: Inceoglu S,Burghardt A,Akbay A,Majumdar S,McLain RF

    更新日期:2005-07-01 00:00:00

  • Similar radiologic lesions of localized Scheuermann's disease of the lumbar spine in twin sisters.

    abstract:SUMMARY OF BACKGROUND DATA:Hereditary and mechanical factors are considered to be the principal etiologic factors of Scheuermann's disease. OBJECTIVES:The authors report identical twins presenting similar lesions of localized lumbar osteochondrosis, which were worse in the one twin that practiced strenuous sports acti...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199404150-00020

    authors: van Linthoudt D,Revel M

    更新日期:1994-04-15 00:00:00

  • Long scoliosis fusion to the sacrum in adults with nonparalytic scoliosis. An improved method.

    abstract::The first 17 adults with nonparalytic scoliosis having long fusion to the sacrum treated with the Luque-Galveston technique were reviewed. There were 3 men and 14 women. Their average age at the time of surgery was 47 years and the mean follow-up period was 42 months. There were no neurologic complications and no pati...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199007000-00007

    authors: Saer EH 3rd,Winter RB,Lonstein JE

    更新日期:1990-07-01 00:00:00

  • Root and spinal cord compression from methylmethacrylate vertebroplasty.

    abstract:STUDY DESIGN:Case report and literature review. OBJECTIVES:Clinicians use methylmethacrylate vertebroplasty to treat vertebral hemangiomas, metastases, and osteoporotic fractures. Cement may leak out of the vertebral body and compress the adjacent spinal cord and nerve roots. We review a case of nerve-root and cord co...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-200107010-00021

    authors: Ratliff J,Nguyen T,Heiss J

    更新日期:2001-07-01 00:00:00

  • Effects of laminectomy and retained extradural foreign body on cauda equina adhesion.

    abstract::The histologic effects of posterior lumbar surgery and retained extradural foreign bodies on the cauda equina were investigated in rats over time. The following four groups of rats were provided: Group 1, sham operation (laminar exposure alone); Group 2, laminectomy alone; Group 3, laminectomy with retained extradural...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199310000-00010

    authors: Yamagami T,Matsui H,Tsuji H,Ichimura K,Sano A

    更新日期:1993-10-01 00:00:00

  • Facet asymmetry in normal vertebral growth: characterization and etiologic theory of scoliosis.

    abstract:STUDY DESIGN:The shape and orientation of the thoracic and lumbar zygapophyseal facets at the T1-L5 level in children were measured and analyzed. OBJECTIVE:To detect the pattern of zygapophyseal facet asymmetry in the thoracic and lumbar spines in children. SUMMARY OF BACKGROUND DATA:Whereas many studies have defined...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e31816b1f83

    authors: Masharawi YM,Peleg S,Albert HB,Dar G,Steingberg N,Medlej B,Abbas J,Salame K,Mirovski Y,Peled N,Hershkovitz I

    更新日期:2008-04-15 00:00:00

  • Necessity of rib head resection for anterior discectomy in the thoracic spine.

    abstract:STUDY DESIGN:The relation between the rib head and the thoracic disc was investigated anatomically. OBJECTIVES:To clarify the necessity of rib head resection in thoracoscopic discectomy using the anterior approach. SUMMARY OF BACKGROUND DATA:When using the transthoracic anterior approach, the rib head must often be r...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000132516.03023.c4

    authors: Moro T,Kikuchi S,Konno S

    更新日期:2004-08-01 00:00:00

  • What works best for whom? An exploratory, subgroup analysis in a randomized, controlled trial on the effectiveness of a workplace intervention in low back pain patients on return to work.

    abstract:STUDY DESIGN:Exploratory subgroup analysis in a randomized controlled trial (RCT). OBJECTIVE:To detect possible moderators in the effectiveness of a workplace intervention in a population of workers with sick leave due to sub acute nonspecific low back pain. SUMMARY OF BACKGROUND DATA:In a recently published RCT, a w...

    journal_title:Spine

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/BRS.0b013e3181a09631

    authors: Steenstra IA,Knol DL,Bongers PM,Anema JR,van Mechelen W,de Vet HC

    更新日期:2009-05-20 00:00:00

  • The Effect of Substance P on an Intervertebral Disc Rat Organ Culture Model.

    abstract:STUDY DESIGN:Laboratory study. OBJECTIVE:Evaluate the effect of substance P (SP) on an intervertebral disc rat organ culture model. SUMMARY OF BACKGROUND DATA:Monolayer cell experiments have demonstrated that exposure intervertebral disc tissue cells to SP leads to upregulation in inflammatory cytokine expression; ho...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000001676

    authors: Koerner JD,Markova DZ,Schroeder GD,Rihn JA,Hilibrand AS,Vaccaro AR,Anderson DG,Kepler CK

    更新日期:2016-12-15 00:00:00

  • Combined anterior and posterior spine fusion in cerebral palsy.

    abstract::Two-staged anterior and posterior spine fusion with instrumentation was evaluated in 17 cerebral palsy patients, ages 11 to 30 years. Functional benefits included improved sitting balance and, in ambulators, no loss of independence. Curve correction averaged 60%. The pseudarthrosis rate of 18% was lower than previousl...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-198211000-00010

    authors: Brown JC,Swank S,Specht L

    更新日期:1982-11-01 00:00:00

  • A study of the cutaneous nerves encountered during thoracoplasty.

    abstract:STUDY DESIGN:Ten cadavers were dissected to describe the cutaneous branches of the dorsal rami nerves that should be identified and protected throughout the thoracoplasty procedure. OBJECTIVE:To identify the anatomic distribution of the cutaneous branches of the dorsal rami in the thoracic spine. SUMMARY OF BACKGROUN...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000253606.82387.96

    authors: Hoernschemeyer D,Farjoodi P,Cheng J,Sponseller P

    更新日期:2007-02-01 00:00:00

  • Impact of Resident Participation on Outcomes After Single-Level Anterior Cervical Diskectomy and Fusion: An Analysis of 3265 Patients from the American College of Surgeons National Surgical Quality Improvement Program Database.

    abstract:STUDY DESIGN:Multicenter retrospective cohort study. OBJECTIVE:To investigate the relationship between resident involvement in the operating room and 30-day complication rates in patients undergoing single-level anterior cervical diskectomy and fusion (ACDF). SUMMARY OF BACKGROUND DATA:Although an integral part of ac...

    journal_title:Spine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/BRS.0000000000001230

    authors: Kim RB,Garcia RM,Smith ZA,Dahdaleh NS

    更新日期:2016-03-01 00:00:00

  • Effects of Training and Overtraining on Intervertebral Disc Proteoglycans.

    abstract:STUDY DESIGN:Animal experimental study. OBJECTIVE:Evaluate the effect of physical activity and overtraining condition on glycosaminoglycan concentration on the intervertebral disc (IVD) using a rat running model. SUMMARY OF BACKGROUND DATA:Some guidelines recommend the implementation of a physical exercise program as...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000002368

    authors: Ueta RHS,Tarini VAF,Franciozi CES,Tamaoki MJS,Medeiros VP,Nader HB,Faloppa F

    更新日期:2018-01-01 00:00:00

  • Detection of Mycobacterium tuberculosis in formaldehyde solution-fixed, paraffin-embedded tissue by polymerase chain reaction in Pott's disease.

    abstract:STUDY DESIGN:Twenty-five formaldehyde solution-fixed, paraffin-embedded tissue blocks from vertebral biopsy specimen materials with presumptive diagnosis of tuberculous spondylitis and nonspecific vertebral osteomyelitis were studied. OBJECTIVES:To evaluate the sensitivity and specificity of polymerase chain reaction ...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199609010-00011

    authors: Berk RH,Yazici M,Atabey N,Ozdamar OS,Pabuccuoglu U,Alici E

    更新日期:1996-09-01 00:00:00

  • A new in vivo animal model to create intervertebral disc degeneration characterized by MRI, radiography, CT/discogram, biochemistry, and histology.

    abstract:STUDY DESIGN:A new in vivo sheep model was developed that produced disc degeneration through the injection of 5-bromodeoxyuridine (BrdU) into the intervertebral disc. This process was studied using magnetic resonance imaging (MRI), radiography, CT/discogram, histology, and biochemistry. OBJECTIVES:To develop a sheep m...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/01.brs.0000259835.31062.3d

    authors: Zhou H,Hou S,Shang W,Wu W,Cheng Y,Mei F,Peng B

    更新日期:2007-04-15 00:00:00

  • Changes in life satisfaction and self-esteem in patients with adolescent idiopathic scoliosis with and without surgical intervention.

    abstract:STUDY DESIGN:Pre-/poststudy comparing surgical and nonsurgical treatment. OBJECTIVE:To identify whether orthopedic spinal surgery can effectively improve life satisfaction and self-esteem in patients with adolescent idiopathic scoliosis. SUMMARY OF BACKGROUND DATA:There have been many studies about the effect of spin...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e3181e0f034

    authors: Zhang J,He D,Gao J,Yu X,Sun H,Chen Z,Li M

    更新日期:2011-04-20 00:00:00

  • Incision of the anulus fibrosus induces nerve root morphologic, vascular, and functional changes. An experimental study.

    abstract:STUDY DESIGN:The effects on nerve root structure, vasculature, and function after incision of the adjacent disc was studied in a dog model. OBJECTIVES:To see if only incision of the disc per se is sufficient for inducing similar changes. SUMMARY OF BACKGROUND DATA:It is well known that nucleus pulposus will induce ne...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-199611150-00002

    authors: Kayama S,Konno S,Olmarker K,Yabuki S,Kikuchi S

    更新日期:1996-11-15 00:00:00

  • Identifying sources of variability in scoliosis classification using a rule-based automated algorithm.

    abstract:STUDY DESIGN:Use of a rule-based automated algorithm to determine sources of variability in radiographic classification. OBJECTIVES:To determine whether unambiguous rules encoded in a computer program would ensure reliable classification. SUMMARY OF BACKGROUND DATA:Reliability problems have been identified in classif...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-200212150-00014

    authors: Stokes IA,Aronsson DD

    更新日期:2002-12-15 00:00:00

  • Randomized controlled trial of specific spinal stabilization exercises and conventional physiotherapy for recurrent low back pain.

    abstract:STUDY DESIGN:Pragmatic, multicentered randomized controlled trial, with 12-month follow-up. OBJECTIVE:To evaluate the effect of adding specific spinal stabilization exercises to conventional physiotherapy for patients with recurrent low back pain (LBP) in the United Kingdom. SUMMARY OF BACKGROUND DATA:Spinal stabiliz...

    journal_title:Spine

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1097/01.brs.0000232787.71938.5d

    authors: Cairns MC,Foster NE,Wright C

    更新日期:2006-09-01 00:00:00

  • Can the interpedicular distance reliably assess the severity of thoracolumbar burst fractures?

    abstract:STUDY DESIGN:Retrospective analysis of 260 patients with acute spine fractures treated at a tertiary trauma center from 1989 to 2009. OBJECTIVE:To correlate the Interpedicular distance (IPD) to the percentage of narrowing of the spinal canal and to the presence of neurological deficit and laminar fracture in thoracolu...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0b013e318237b0cf

    authors: Caffaro MF,Avanzi O

    更新日期:2012-02-15 00:00:00

  • The effect of serial growing rod lengthening on the sagittal profile and pelvic parameters in early-onset scoliosis.

    abstract:STUDY DESIGN:Retrospective case series. OBJECTIVE:To report the effect of repeated growing rod (GR) lengthenings on the sagittal and pelvic profile in patients with early-onset scoliosis. SUMMARY OF BACKGROUND DATA:Posterior distraction-based GRs have gained popularity as a technique for the surgical management of ea...

    journal_title:Spine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/BRS.0000000000000565

    authors: Shah SA,Karatas AF,Dhawale AA,Dede O,Mundis GM Jr,Holmes L Jr,Yorgova P,Neiss G,Johnston CE,Emans JB,Thompson GH,Pawelek JB,Akbarnia BA,Growing Spine Study Group.

    更新日期:2014-10-15 00:00:00

  • External Validation of the Adult Spinal Deformity (ASD) Frailty Index (ASD-FI) in the Scoli-RISK-1 Patient Database.

    abstract:STUDY DESIGN:Analysis of a prospective multicenter database. OBJECTIVE:To assess the ability of the recently created Adult Spinal Deformity (ASD) Frailty Index (ASD-FI) to predict odds of major complications and length of hospital stay for patients who had more severe preoperative deformity and underwent more invasive...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000002717

    authors: Miller EK,Lenke LG,Neuman BJ,Sciubba DM,Kebaish KM,Smith JS,Qiu Y,Dahl BT,Pellisé F,Matsuyama Y,Carreon LY,Fehlings MG,Cheung KM,Lewis S,Dekutoski MB,Schwab FJ,Boachie-Adjei O,Mehdian H,Bess S,Shaffrey CI,Ames CP

    更新日期:2018-10-15 00:00:00

  • The mechanical function of the lumbar apophyseal joints.

    abstract::Experiments were carried out on cadaveric lumbar spines to determine the mechanical function of the apophyseal joints. It was found that they resist most of the intervertebral shear force and share in resisting the intervertebral compressive force, but only in lordotic postures. They prevent excessive movement from da...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/00007632-198304000-00017

    authors: Adams MA,Hutton WC

    更新日期:1983-04-01 00:00:00

  • Congenital dumbbell neuroblastoma.

    abstract:STUDY DESIGN:New babies with neurologic deficits resulting from intraspinal extension of congenital neuroblastoma is very unusual. Two patients with congenital dumbbell neuroblastoma with paraplegia are described, and 16 other cases are reviewed. OBJECTIVE:To study the clinical features and prognosis of congenital dum...

    journal_title:Spine

    pub_type: 杂志文章,评审

    doi:10.1097/00007632-199506000-00019

    authors: Shimada Y,Sato K,Abe E,Suzuki T,Ishihara Y

    更新日期:1995-06-01 00:00:00

  • Predictors of Vertebral Artery Injury in Isolated C2 Fractures Based on Fracture Morphology Using CT Angiography.

    abstract:STUDY DESIGN:Retrospective database analysis. OBJECTIVE:To determine C2 fracture patterns associated with vertebral artery injury (VAI) as assessed by computed tomography angiography. SUMMARY OF BACKGROUND DATA:The incidence of C2 fracture hospitalizations has increased significantly in the last decade. The vertebral...

    journal_title:Spine

    pub_type: 杂志文章

    doi:10.1097/BRS.0000000000000893

    authors: Durand D,Wu X,Kalra VB,Abbed KM,Malhotra A

    更新日期:2015-06-15 00:00:00