Abstract:
STUDY DESIGN:Twenty-five formaldehyde solution-fixed, paraffin-embedded tissue blocks from vertebral biopsy specimen materials with presumptive diagnosis of tuberculous spondylitis and nonspecific vertebral osteomyelitis were studied. OBJECTIVES:To evaluate the sensitivity and specificity of polymerase chain reaction in detecting Mycobacterium tuberculosis in formaldehyde solution-fixed, paraffin-embedded tissue samples from histologically proved tuberculous spondylitis. SUMMARY OF BACKGROUND DATA:Diagnosis of a mycobacterial infection is a long and tedious process; because of the slow growth rate of mycobacteria on solid media, identification and antibiotic sensitivity testing can take up to 10 weeks, but the sensitivity of culture can be as low as 50%. Direct microscopy is insensitive because clinical samples may contain only few organisms. Recently, polymerase chain reaction has been applied in the rapid amplification and identification of many organisms, including mycobacteria. METHODS:The DNAs were extracted from 25 paraffin-embedded tissue blocks. An insertion element IS 6110 (Integrated DNA Tec. Inc., Corrallville, IA), a DNA sequence unique to Mycobacterium complex (M. tuberculosis and the subspecies Mycobacterium bovis), was amplified by polymerase chain reaction. Polymerase chain reaction results were compared with those of Mycobacterium culture, acid-fast bacilli staining, and histologic findings. RESULTS:Polymerase chain reaction was positive in 18 cases of 19 tuberculous spondylitis. Three of the polymerase chain reaction test results were positive with concomitant negative culture and positive acid-fast bacilli staining. There were six chronic nonspecific infections, and polymerase chain reaction results were negative in five cases; in the single positive case, DNA amplification results remained positive even after three repeated tests. CONCLUSION:Polymerase chain reaction has a sensitivity of 94.7%, specificity of 83.3%, positive predictive value of 94.7%, and a negative predictive value of 83.3%. Accuracy was calculated as 92%.
journal_name
Spine (Phila Pa 1976)journal_title
Spineauthors
Berk RH,Yazici M,Atabey N,Ozdamar OS,Pabuccuoglu U,Alici Edoi
10.1097/00007632-199609010-00011subject
Has Abstractpub_date
1996-09-01 00:00:00pages
1991-5issue
17eissn
0362-2436issn
1528-1159journal_volume
21pub_type
杂志文章相关文献
SPINE文献大全abstract:STUDY DESIGN:This case report shows primary natural killer (NK)/T-cell lymphoma originating in the spine as the first case of successful treatment with autologous stem cell transplantation, along with case presentation and literature review. OBJECTIVE:For the proper treatment of this spinal tumor, the pathologic confi...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3182407b0b
更新日期:2012-05-01 00:00:00
abstract:STUDY DESIGN:To investigate the incidence of acute neurologic complications of use of sublaminar wires with third-generation spine instrumentation for the treatment of idiopathic scoliosis. OBJECTIVES:To assess the safety of sublaminar wires in the surgical treatment of idiopathic scoliosis. SUMMARY OF BACKGROUND DAT...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-200003150-00007
更新日期:2000-03-15 00:00:00
abstract:STUDY DESIGN:This study analyzed the correlation between the atlantoaxial instability (especially sagittal plane rotatory instability) and myelopathy. Thirty-four patients with os odontoideum were reviewed in clinical symptoms and radiographs. OBJECTIVES:The results were correlated to provide a rationale for the genes...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199606150-00007
更新日期:1996-06-15 00:00:00
abstract:STUDY DESIGN:Case report. OBJECTIVE:To report the occurrence of a rare complication following revision of a spinal cord stimulator. SUMMARY OF BACKGROUND DATA:Puncture of the dura with placement of an electrode within the spinal cord has not been reported as a complication of spinal cord stimulation. METHODS:A patie...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e31814541dc
更新日期:2007-09-01 00:00:00
abstract::A retrospective review of 326 female patients with untreated idiopathic scoliosis was performed to determine what factors might be useful in predicting the risk of curve progression. Components such as family history, height-weight ratio, lumbosacral transitional anomalies, kyphosis, lordosis, and trunk balance were n...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-198610000-00003
更新日期:1986-10-01 00:00:00
abstract:STUDY DESIGN:A large animal study comparing interbody fusion of a bioresorbable scaffold loaded with either low-dose recombinant human bone morphogenetic protein 2 (rhBMP-2) or bone marrow-derived multipotent stromal cells (BMSCs). OBJECTIVE:To compare the quality of fusion resulting from implantation of medical grade...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e31822576a4
更新日期:2011-10-01 00:00:00
abstract:STUDY DESIGN:The study was based on porcine posterolateral fusion model. OBJECTIVE:The study aims to prove that polyelectrolyte complex (PEC) carrier could enhance the efficacy and safety profile of bone morphogenetic protein-2 (BMP-2). SUMMARY OF BACKGROUND DATA:BMP-2 was introduced to enhance posterolateral fusion;...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000001543
更新日期:2016-08-01 00:00:00
abstract:STUDY DESIGN:Destructive compression tests and finite element analyses were conducted to investigate the biomechanical strength at the graft-endplate interface in anterior cervical fusion. OBJECTIVES:To investigate the effect of endplate thickness, endplate holes, and bone mineral density of the vertebral body on the ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-200104150-00021
更新日期:2001-04-15 00:00:00
abstract:STUDY DESIGN:The authors have developed technique of using anterior neck derivations to record posterior tibial nerve N28 during operative somatosensory-evoked potential monitoring. OBJECTIVE:This prospective study of 10 patients compared the ease of application of electrodes and stability of waveforms with "tradition...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199504000-00008
更新日期:1995-04-01 00:00:00
abstract:STUDY DESIGN:Review article. OBJECTIVE:To explain why the evidence that compensation-related factors lead to worse health outcomes is not compelling, either in general, or in the specific case of whiplash. SUMMARY OF BACKGROUND DATA:There is a common view that compensation-related factors lead to worse health outcome...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3182388411
更新日期:2011-12-01 00:00:00
abstract:STUDY DESIGN:Systematic review. OBJECTIVE:To identify, describe, and evaluate common outcome measures in patients with chronic low back pain (CLBP). SUMMARY OF BACKGROUND DATA:The treatment of CLBP has been associated with multiple clinical challenges. Further complicating this is the myriad of outcome scores used to...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e31822ef74d
更新日期:2011-10-01 00:00:00
abstract:STUDY DESIGN:Human intervertebral disc cells were cultured in monolayer and treated with adenovirus-containing marker genes to determine the susceptibility of the cells to adenovirus-mediated gene transfer. OBJECTIVES:To test the efficacy of the adenovirus-mediated gene transfer technique for transferring exogenous ge...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-200010150-00006
更新日期:2000-10-15 00:00:00
abstract:STUDY DESIGN:We performed a retrospective review of 126 cases of infectious spondylodiscitis over a 4-year period. OBJECTIVE:Differentiation between pyogenic spondylodiscitis (PS) and tuberculous spondylodiscitis (TS) is essential for deciding on the appropriate therapeutic regimen. The aim of this study was to compar...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181e04dd3
更新日期:2010-10-01 00:00:00
abstract:STUDY DESIGN:To determine the effect of cage/spacer stiffness on the stresses in the bone graft and cage subsidence. OBJECTIVE:To investigate the effect of cage stiffness on the biomechanics of the fused segment in the lumbar region using finite element analysis. SUMMARY OF BACKGROUND DATA:There are a wide variety of...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000250177.84168.ba
更新日期:2006-12-15 00:00:00
abstract:STUDY DESIGN:The Low Back Pain Symptom Check List identifies psychological disturbance in patients with low back pain. This report traces the development of a translation for Hispanic populations. OBJECTIVE:The study explores the reliability and assesses the equivalence of the translation in providing pain and psychol...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199405000-00009
更新日期:1994-05-01 00:00:00
abstract:STUDY DESIGN:We compared anterior and posterior surgery for cervical myelopathy resulting from ossification of the posterior longitudinal ligament. Surgical techniques, based on shape and distribution of ossification of the posterior longitudinal ligament, were divided into four technical phases. OBJECTIVES:Long-term ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199510001-00012
更新日期:1995-10-15 00:00:00
abstract:STUDY DESIGN:A retrospective radiological study. OBJECTIVE:To analyze the course of intra-axial vertebral artery (IAVA) and evaluate the relationship between the 3-dimensional (3D) courses for IAVA with respect to safe trajectory for C2 pedicle screw (C2PS). SUMMARY OF BACKGROUND DATA:The VA at the level of C2 has a ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000000418
更新日期:2014-08-01 00:00:00
abstract:STUDY DESIGN:Coculture assays of the migration and interaction of human intervertebral disc cells and chick sensory nerves on alternate substrata of collagen and aggrecan. OBJECTIVE:To examine the effects of aggrecan on disc cell migration, how disc cells and sensory nerves interact, and whether disc cells affect prev...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000217669.04903.61
更新日期:2006-05-15 00:00:00
abstract:STUDY DESIGN:An in vitro immature bovine study of thoracic spine fixation using shape memory alloy for minimally invasive adolescent scoliosis treatment. OBJECTIVE:The purpose of this study was to detect differences in thoracic spine range of motion due to the placement of spinal staples manufactured from a common sha...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000259073.16006.ed
更新日期:2007-04-01 00:00:00
abstract:STUDY DESIGN:Case report and literature review. OBJECTIVE:To describe a unique case of recurrent cerebral embolisms with "bilateral" atlantoaxial rotational vertebral artery occlusion (RVAO), and review the literature on this rare entity. SUMMARY OF BACKGROUND DATA:The few reports of bilateral bow hunter's stroke (BH...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0000000000000341
更新日期:2014-06-15 00:00:00
abstract:STUDY DESIGN:A technical report of fluoroscopically assisted percutaneous translaminar facet screw fixation after anterior lumbar interbody fusion (ALIF). OBJECTIVES:To describe a minimally invasive translaminar facet screw fixation technique that was modified from the Magerl method, and to assess its technical feasib...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000157473.17313.6f
更新日期:2005-04-01 00:00:00
abstract:STUDY DESIGN:Case report and clinical discussion. OBJECTIVE:We intend to report a very rare case of a giant spinal hemangioma causing myelopathy. SUMMARY OF BACKGROUND DATA:Multilevel symptomatic spinal hemangiomas causing acute neurologic symptoms are rare disorders. We found only sporadic reports in English literat...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181a4e4b8
更新日期:2009-06-15 00:00:00
abstract:STUDY DESIGN:This study analyzed the sizes and angles of vertebrae and discs in Chinese subjects. Lateral lumbar spine radiograph films were taken of subjects in upright and 60 degrees flexion positions to evaluate disc height and angle changes. OBJECTIVES:This study sought to gather data regarding the contribution of...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199407000-00013
更新日期:1994-07-01 00:00:00
abstract:STUDY DESIGN:Retrospective review of anterior and posterior fusions for treatment of adolescent idiopathic thoracic scoliosis. OBJECTIVES:To delineate the best factors determining final lumbar curve magnitude in patients with adolescent idiopathic scoliosis undergoing a selective thoracic anterior or posterior spinal ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000106488.51299.75
更新日期:2004-02-01 00:00:00
abstract:STUDY DESIGN:Automatic measurement of Cobb angle in patients with scoliosis. OBJECTIVE:To test the accuracy of an automatic Cobb angle determination method from frontal radiographical images. SUMMARY OF BACKGROUND DATA:Thirty-six frontal radiographical images of patients with scoliosis. METHODS:A modified charged pa...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3182a0c7c3
更新日期:2013-09-15 00:00:00
abstract:STUDY DESIGN:A systematic review of randomized controlled trials. BACKGROUND:Although several rehabilitation programs, physical fitness programs, or protocols regarding instruction for patients to return to work after lumbar disc surgery have been suggested, little is known about the efficacy of these treatments, and ...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/01.BRS.0000042520.62951.28
更新日期:2003-02-01 00:00:00
abstract:STUDY DESIGN:A retrospective study was performed of 182 revisions on failed back surgery syndrome from the years 1965 to 1990. OBJECTIVE:To analyze the reasons for failure of primary discectomy, the outcome of the revisions, and factors that influenced those outcomes. SUMMARY OF BACKGROUND DATA:The reported reinterve...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199603010-00017
更新日期:1996-03-01 00:00:00
abstract:STUDY DESIGN:A retrospective study. OBJECTIVE:To investigate whether the mismatch between proximal junctional angle (PJA) and the proximal rod contouring contributed to the occurrence of postoperative proximal junctional kyphosis (PJK) in degenerative scoliosis. SUMMARY OF BACKGROUND DATA:PJK is one of the complicati...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000001883
更新日期:2017-03-01 00:00:00
abstract:STUDY DESIGN:A case report and review of the literature are presented. OBJECTIVE:To describe an unusual location for a cervical synovial cyst and to review characteristic presentations and findings of synovial cysts in the cervical spine. SUMMARY OF BACKGROUND DATA:Synovial cysts in the cervical spine are rare. To da...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.BRS.0000090501.42188.2A
更新日期:2003-09-01 00:00:00
abstract:STUDY DESIGN:Systematic review, ambispective analysis of observational data. OBJECTIVE:To make recommendations as to whether or not urgent surgical decompression is ever indicated as the optimal treatment for enhancing neurologic recovery in a patient with acute central cord injury without fracture or instability. SU...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3181f32a44
更新日期:2010-10-01 00:00:00