Abstract：
:The long terminal repeat retrotransposon, Magnaporthe gypsy-like element (MAGGY), has been shown to be targeted for cytosine methylation in a subset of Magnaporthe oryzae field isolates. Analysis of the F1 progeny from a genetic cross between methylation-proficient (Br48) and methylation-deficient (GFSI1-7-2) isolates revealed that methylation of the MAGGY element was governed by a single dominant gene. Positional cloning followed by gene disruption and complementation experiments revealed that the responsible gene was the DNA methyltransferase, MoDMT1, an ortholog of Neurospora crassa Dim-2. A survey of MAGGY methylation in 60 Magnaporthe field isolates revealed that 42 isolates from rice, common millet, wheat, finger millet, and buffelgrass were methylation proficient while 18 isolates from foxtail millet, green bristlegrass, Japanese panicgrass, torpedo grass, Guinea grass, and crabgrass were methylation deficient. Phenotypic analyses showed that MoDMT1 plays no major role in development and pathogenicity of the fungus. Quantitative polymerase chain reaction analysis showed that the average copy number of genomic MAGGY elements was not significantly different between methylation-deficient and -proficient field isolates even though the levels of MAGGY transcript were generally higher in the former group. MoDMT1 gene sequences in the methylation-deficient isolates suggested that at least three independent mutations were responsible for the loss of MoDMT1 function. Overall, our data suggest that MoDMT1 is not essential for the natural life cycle of the fungus and raise the possibility that the genus Magnaporthe may be losing the mechanism of DNA methylation on the evolutionary time scale.
journal_name
Geneticsjournal_title
Geneticsauthors
Ikeda K,Van Vu B,Kadotani N,Tanaka M,Murata T,Shiina K,Chuma I,Tosa Y,Nakayashiki Hdoi
10.1534/genetics.113.155978subject
Has Abstractpub_date
2013-11-01 00:00:00pages
845-55issue
3eissn
0016-6731issn
1943-2631pii
genetics.113.155978journal_volume
195pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The complex structure and repetitive nature of eukaryotic ribosomal DNA (rDNA) is a challenge for genome assembly, thus the consequences of sequence variation in rDNA remain unexplored. However, renewed interest in the role that rDNA variation may play in diverse cellular functions, aside from ribosome production, hig...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302351
更新日期：2019-09-01 00:00:00
abstract：:Adaptive dynamics formalism demonstrates that, in a constant environment, a continuous trait may first converge to a singular point followed by spontaneous transition from a unimodal trait distribution into a bimodal one, which is called "evolutionary branching." Most previous analyses of evolutionary branching have b...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.144980
更新日期：2013-01-01 00:00:00
abstract：:The St. Thomas Hospital (STH) rabbit has been previously shown to have a Mendelian form of hypertriglyceridemia, accompanied by accelerated atherosclerosis, and these animals may serve as a useful model for human dyslipoproteinemia syndromes. Here we describe the establishment of a new colony of these STH animals, and...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Biosynthesis of most peptide hormones and neuropeptides requires proteolytic excision of the active peptide from inactive proprotein precursors, an activity carried out by subtilisin-like proprotein convertases (SPCs) in constitutive or regulated secretory pathways. The Drosophila amontillado (amon) gene encodes a hom...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:In the present study, we investigated intrachromosomal homologous recombination in a murine hybridoma in which the recipient for recombination, the haploid, endogenous chromosomal immunoglobulin mu-gene bearing a mutation in the constant (Cmu) region, was separated from the integrated single copy wild-type donor Cmu r...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:The determination of segment identity in Drosophila melanogaster appears to be controlled by a small number of genes. In order to identity new components in the process, we have systematically screened the autosomal complement for loci that show a dosage-dependent interaction with mutations in previously characterized...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Interactions with bacteria play a major role in immune responses, ecology, and evolution of all animals, but they have been neglected until recently in the case of C. elegans. We report a genetic investigation of the interaction of C. elegans with the nematode-specific pathogen Microbacterium nematophilum, which colon...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.045716
更新日期：2005-11-01 00:00:00
abstract：:In order to examine the mechanisms of mutagenesis by a bulky DNA lesion at the guanine N7 position, the replicative form DNA of phage M13AB28 (mp8 without the amber codons in phage genes) was modified in vitro with aflatoxin B1-2,3-dichloride and transfected into appropriate Escherichia coli cells. Forward mutations i...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:We show that major chromosomal rearrangements can occur upon T-DNA transformation of Arabidopsis thaliana. In the ACL4 line, two T-DNA insertion loci were found; one is a tandem T-DNA insert in a head-to-head orientation, and the other is a truncated insert with only the left part of the T-region. The four flanking DN...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:Stress-induced mutagenesis is a collection of mechanisms observed in bacterial, yeast, and human cells in which adverse conditions provoke mutagenesis, often under the control of stress responses. Control of mutagenesis by stress responses may accelerate evolution specifically when cells are maladapted to their enviro...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.100735
更新日期：2009-05-01 00:00:00
abstract：:A computer system for the storage and processing of microbial meiotic data has been developed. Based on the language Fortran 4, the program retrieves relevant data and determines the order, map distances, and coefficient of coincidence for any three-gene group. Meiotic data from Aspergillus nidulans were used to test ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-12-01 00:00:00
abstract：:Transposable element activity is repressed in the germline in animals by PIWI-interacting RNAs (piRNAs), a class of small RNAs produced by genomic loci mostly composed of TE sequences. The mechanism of induction of piRNA production by these loci is still enigmatic. We have shown that, in Drosophila melanogaster, a clu...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.180307
更新日期：2015-12-01 00:00:00
abstract：:We investigated genetic polymorphism in the Caenorhabditis elegans srh and str chemoreceptor gene families, each of which consists of approximately 300 genes encoding seven-pass G-protein-coupled receptors. Almost one-third of the genes in each family are annotated as pseudogenes because of apparent functional defects...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.035329
更新日期：2005-04-01 00:00:00
abstract：:A genetic model with additive-dominance effects and genotype x environment interactions is presented for quantitative traits with time-dependent measures. The genetic model for phenotypic means at time t conditional on phenotypic means measured at previous time (t-1) is defined. Statistical methods are proposed for an...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:The Dissociation (Ds) mutant, Bz-wm, of the maize bronze-1 (bz) locus conditions a leaky phenotype. Plants carrying this mutant allele synthesize a low amount of an altered Bz gene product, which leads to reduced anthocyanin pigmentation in the seed. The molecular analysis reported here shows that the Bz-wm mutant has...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:RNAs are localized by microtubule-based pathways to both the anterior and posterior poles of the developing Drosophila oocyte. We describe a new gene, wispy, required for localization of mRNAs to both poles of the egg. Embryos from wispy mothers arrest development after abnormal oocyte meiosis and failure of pronuclei...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Site-specific recombinases are widely used tools for analysis of genetics, development, and cell biology, and many schemes have been devised to alter gene expression by recombinase-mediated DNA rearrangements. Because the FRT and lox target sites for the commonly used FLP and Cre recombinases are asymmetrical, and mus...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303394
更新日期：2020-08-01 00:00:00
abstract：:We have studied the deletion of inverted repeats cloned into the EcoRI site within the CAT gene of plasmid pBR325. A cloned inverted repeat constitutes a palindrome that includes both EcoRI sites flanking the insert. In addition, the two EcoRI sites represent direct repeats flanking a region of palindromic symmetry. A...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:An inbreeding matrix is defined for populations with overlapping generations. In the short term it can be expressed in terms of a matrix specifying the passage of genes between the different age groups (and sexes) and a diagonal matrix whose elements depend on the number of individuals in each age group. Formulae for ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-11-01 00:00:00
abstract：:Chromosomal double-strand breaks (DSBs) that have only one end with homology to a donor duplex undergo repair by strand invasion followed by replication to the chromosome terminus (break-induced replication, BIR). Using a transformation-based assay system, it was previously shown that BIR could occur by several rounds...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.162297
更新日期：2014-04-01 00:00:00
abstract：:In the yeast Saccharomyces cerevisiae, rim1, 8, 9, or 13 mutations cause four phenotypes: poor growth at low temperature, altered colony morphology, inefficient sporulation due to reduced expression of the meiotic activator IME1, and, as shown here, defective invasive growth. In this report, we have determined the rel...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:The central and marginal populations of D. robusta differ greatly in the level of inversion polymorphism; the marginal populations are monomorphic or nearly so and the central populations are highly polymorphic. This paper presents the frequencies of alleles at forty gene loci in various populations of D. robusta, stu...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-10-01 00:00:00
abstract：:A reversion study of EMS- or ICR170-induced ascospore color mutants in Ascobolus immersus is reported. Twenty-three new intragenic suppressors were isolated within the b2 locus. These are localized within three distinct groups on a genetic fine-structure map containing 21 identifiable sites. The pattern of reversion i...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
abstract：:I have studied nucleotide polymorphism and linkage disequilibrium using multilocus data from 77 fragments, with an average length of fragments of 550 bp, in the deciduous tree Populus tremula (Salicaceae). The frequency spectrum across loci showed a modest excess of mutations segregating at low frequency and a marked ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.090431
更新日期：2008-09-01 00:00:00
abstract：:The rate of decline in reproductive fitness in populations of Drosophila melanogaster inbred at an initial rate of approximately 1% per generation has been investigated under both competitive and noncompetitive conditions. Breeding population size was variable in the inbred lines with an estimated harmonic mean of 66....
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Genetic information can be altered by base substitutions, frameshift mutations, and addition or deletion of nucleotides. Deletions represent an important class of genetic aberration occurring at DNA sequences where it is often possible to predict the existence of intermediates of mutation. Instability within tracts of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:Genetically marked P elements inserted into the subtelomeric satellites of Drosophila show repression and variegation of the reporter gene. One such white+ reporter, inserted between the subtelomeric satellite and the terminal HeT-A array in the left arm of chromosome 2 (2L), is sensitive to its context; changes in th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:We describe a method for co-estimating r = C/mu (where C is the per-site recombination rate and mu is the per-site neutral mutation rate) and Theta = 4N(e)mu (where N(e) is the effective population size) from a population sample of molecular data. The technique is Metropolis-Hastings sampling: we explore a large numbe...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:The challenges of breeding autotetraploid potato (Solanum tuberosum) have motivated the development of alternative breeding strategies. A common approach is to obtain uniparental dihaploids from a tetraploid of interest through pollination with S. tuberosum Andigenum Group (formerly S. phureja) cultivars. The mechanis...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302843
更新日期：2020-02-01 00:00:00
abstract：:The rate of microsatellite mutation is dependent upon both the allele length and the repeat motif, but the exact nature of this relationship is still unknown. We analyzed data on the inheritance of human Y-chromosomal microsatellites in father-son duos, taken from 24 published reports and comprising 15,285 directly ob...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.132308
更新日期：2011-12-01 00:00:00
