Abstract:
:Pancreatic cancer is the fourth most common cause of cancer-related mortality. In the present study, we employed 2 types of therapeutic stem cells expressing cytosine deaminase (CD) with or without human interferon-β (IFN‑β), HB1.F3.CD and HB1.F3.CD.IFN-β cells, respectively, to selectively treat pancreatic cancer. The CD gene converts the non-toxic prodrug, 5-flurorocytosine (5-FC), into the toxic agent, 5-fluorouracil (5-FU). In addition, human IFN-β is a potent cytokine that has antitumor effects. To generate a xenograft mouse model, PANC-1 cells (2x10(6)/mouse) cultured in DMEM containing 10% FBS were mixed with Matrigel and were subcutaneously injected into Balb/c nu/nu mice. In the migration assay, the stem cells expressing the CD or IFN-β gene effectively migrated toward the pancreatic cancer cells, suggesting the presence of chemoattractant factors secreted by the pancreatic tumors. In the co-culture and MTT assay, antitumor activity of the therapeutic stem cells was observed in the presence of 5-FC was shown that the growth of PANC-1 cells was inhibited. Furthermore, these effects were confirmed in the xenograft mouse model bearing tumors originating from PANC-1 cells. Analyses by histological and fluorescence microscopy showed that treatment with the stem cells resulted in the inhibition of pancreatic cancer growth in the presence of 5-FC. Taken together, these results indicate that stem cells expressing the CD and/or IFN-β gene can be used to effectively treat pancreatic cancer and reduce the side-effects associated with conventional therapies.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Kim DJ,Yi BR,Lee HR,Kim SU,Choi KCdoi
10.3892/or.2013.2564subject
Has Abstractpub_date
2013-09-01 00:00:00pages
1129-36issue
3eissn
1021-335Xissn
1791-2431journal_volume
30pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Histamine receptor H3 (HRH3) is mainly expressed in the central nervous system, where it is involved in the regulation of the release of various neurotransmitters in the brain. Recent studies have demonstrated that the expression of HRH3 is upregulated in several types of cancer. However, the functional effect of HRH3...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7119
更新日期:2019-06-01 00:00:00
abstract::The cancer stem cell hypothesis suggests that mutated melanocyte stem cells are present in skin as precursors of melanoma cells. Nestin and CD133 have been described as markers of melanocytic stem cells. The aim of this study was to establish if melanocytic stem cells could have a prognostic significance in melanoma p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::MicroRNAs (miRs) have emerged as prospective tools for human cancer therapy, including hepatocellular carcinoma (HCC) therapy. Previous studies have suggested that miR-381 functions as oncogenic or tumor-suppressive miRs in other cancer types. However, the role of miR-381 in HCC remains unknown. The present study inve...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4491
更新日期:2016-03-01 00:00:00
abstract::The inhibitor of differentiation/DNA binding 1 (Id-1), a negative regulator of basic helix-loop-helix transcription factors, plays an important role in the regulation of cell proliferation and differentiation. We examined the Id-1 expression by immunohistochemistry in 9 adenomas, 79 primary colorectal adenocarcinomas ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
abstract::Honokiol (HNK), a potential antitumor compound, has been widely studied in recent years. It induces apoptosis and affects autophagy in cancer cells, yet the mechanism of its antitumor efficacy remains obscure. Chloroquine (CQ), an autophagy inhibitor, is often applied to sensitize antitumor drugs in clinical trials. H...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4091
更新日期:2015-09-01 00:00:00
abstract::Frizzled2 (Fzd2) is a receptor for wingless-type MMTV integration site family members (Wnts), the aberrant overexpression of which has been noted to contribute to cancer metastasis. The present study was performed to characterize the role of Fzd2 in the migration and invasion of oral squamous cell carcinomas (OSCC) in...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4285
更新日期:2015-12-01 00:00:00
abstract::Autophagy is an intracellular multi-step catabolic degradation process that involves the degradation and recycling of cellular proteins and cytoplasmic damaged organelles to maintain cellular homeostasis and reduction of metabolic stress. Numerous studies have indicated the importance of autophagy in cancer, but the r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4302
更新日期:2015-12-01 00:00:00
abstract::By combining suppression subtractive hybridization and microarray to examine gene expressions between metastatic and non-metastatic non-small cell lung cancer (NSCLC), we have identified differential expression spectra of matrix metalloproteinases (MMP). Among MMPs, expressions of MMP-13, -14, -15 and -24 decreased, t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::Tumor metastasis is one of the causes for the high mortality rate of prostate cancer (PCa) patients, yet the molecular mechanisms of PCa metastasis are not fully understood. In our previous studies, we found that PSMA suppresses the metastasis of PCa, yet the underlying mechanism remains unknown. To identify the genes...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2656
更新日期:2013-10-01 00:00:00
abstract::Hydrogen sulfide (H2S) participates in multifarious physiological and pathophysiologic progresses of cancer both in vitro and in vivo. We have previously demonstrated that exogenous H2S promoted liver cancer cells proliferation/anti‑apoptosis/angiogenesis/migration effects via amplifying the activation of NF-κB pathwa...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4248
更新日期:2015-11-01 00:00:00
abstract::The multi-target kinase inhibitor sorafenib has been approved for the treatment of patients with advanced differentiated thyroid cancer. However, different sensitivities to sorafenib have been observed, and few patients have benefited from sorafenib treatment in the long term. In the event of acquired resistance to so...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.6118
更新日期:2018-02-01 00:00:00
abstract::Chemokines are small chemotactic cytokines that can induce the migration of leukocytes, activate inflammatory/immune responses and have recently been implicated in the regulation of tumor growth and organ-specific spread. In this setting, the macrophage inflammatory protein-1alpha (CCL3) chemokine displays a diversity...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::Mammary stem cells (MSCs) are the progenitor population for human breast epithelia. MSCs give rise during mammary gland development to estrogen receptor (ER)-negative basal cells and the ER- luminal progenitor (LP) population which maintains ER+ and ER- luminal cells. The MSC population is expanded and tumorigenic in ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4003
更新日期:2015-07-01 00:00:00
abstract::For deveplopment and function of the lung, progesterone (Prog) fulfils important roles. In a recent report, immunolocalization of Prog and estrogen receptors in non-small cell lung carcinomas were examined and it was shown that the Prog receptor might be a potent prognostic factor. In the present study, a cell line wi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-09-01 00:00:00
abstract::Differentiation of breast cancer has been receiving increasing attention in attempts to minimize the numbers of excisional biopsies for benign tumors. Doppler spectral parameters, such as peak systolic velocity (PSV) and pulsatility index (PI), have been used but these are unable to differentiate breast cancers and be...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Platinum compounds and vinorelbine (VNB) are active in the treatment of non-small cell lung cancer (NSCLC), but moderate toxicity has been reported. The aims of the present study were to assess activity and tolerability of low dose carboplatin (CBDCA): 4.5 AUC according to Calvert formula on day 1, combined with vinor...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:10.3892/or.8.2.381
更新日期:2001-03-01 00:00:00
abstract::Casticin, one of the main components from Fructus Viticis, has been reported to inhibit the growth of various cancer cells, including the human cervical cancer cell line HeLa. The purpose of this study was to examine the apoptotic activity and molecular mechanism of casticin action on human cervical cancer cells. The ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1367
更新日期:2011-11-01 00:00:00
abstract::We investigated whether or not the mitochondrial genotypes affect radiation-induced micronucleus (MN) formation. For that purpose, the rho+, KT1 and rho0 human osteosarcoma cell lines were used, which carry the wild-type mitochondrial DNA (mtDNA), the tRNALys mutant mtDNA and no mtDNA, respectively. Despite no signifi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.3.615
更新日期:2001-05-01 00:00:00
abstract::Effective therapy for advanced hepatocellular carcinoma (HCC) is lacking. Conventional chemotherapy was judged to be ineffective. We previously demonstrated that the histone deacetylase inhibitor Trichostatin A (TSA) blocks growth of HCC cells in vitro. The anti-tumoral effect of a combination of more than 2 classes o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::We have recently noticed an accidental error in part of a figure which appeared in the above‑mentioned article. In Fig. 3A, the image for the HGC27‑pEF, 15 h panel was mistakenly replicated as the HGC27‑KD, 0 h panel in the same figure, and the AGS‑pEF, 15 h and AGS KD, 0 h panels were mistakenly switched with each ot...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6269
更新日期:2018-04-01 00:00:00
abstract::The present study evaluated activity and toxicity of modulated doses of gemcitabine associated to oxaliplatin in patients with secondary CIRS and with locally advanced pancreatic adenocarcinoma (LAPC) and metastatic pancreatic adenocarcinoma (MPC). Since January 2006, untreated LAPC and MPC patients have been assessed...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:10.3892/or.2011.1517
更新日期:2012-02-01 00:00:00
abstract::Glioblastoma (GBM) is the most common type of primary central nervous system tumor in adults, which has high mortality and morbidity rates, and short survival time, namely <15 months after the diagnosis and application of standard therapy, which includes surgery, radiation therapy and chemotherapy; thus, novel therape...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7472
更新日期:2020-03-01 00:00:00
abstract::Previous studies have shown that S100P contributes to the development of a number of tumors. However, its prognostic significance in colorectal cancer (CRC) has not been demonstrated. This study aimed to confirm the expression of S100P in colorectal cancer as well as the epigenetic mechanism underlying its gene expres...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1794
更新日期:2012-07-01 00:00:00
abstract::The 5-year survival rate of nasopharyngeal carcinoma (NPC) is still disappointing despite the much improved technologies in its treatment. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) can selectively induce apoptosis in most tumor cells while sparing normal cells....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1423
更新日期:2011-12-01 00:00:00
abstract::The aim of the present study was to evaluate human epididymis protein 4 (HE4) as a marker of epithelial ovarian cancer (EOC) relapse and the combination of this biomarker with contrast-enhanced high-resolution multidetector row computed tomography CE CT imaging to impove the monitoring of EOC patients. Twenty-one pati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2682
更新日期:2013-11-01 00:00:00
abstract::Acquisition of resistance to paclitaxel is a major obstacle to successful treatment of breast cancer patients, but the molecular mechanisms underlying the development of drug resistance remain largely unclear. The aim of the present study was to investigate the role and mechanism of action of miR‑200c‑3p in the resist...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6735
更新日期:2018-12-01 00:00:00
abstract::Thrombospondin-1 (TSP1) is known to possess tumor suppressor functions. In contradiction, TSP1 enhances the stromal vascularization and growth of certain cancers. A cell adhesion receptor, CD36, has been shown to interact with a ligand TSP1. We studied how CD36 affects the growth of the osteosarcoma cell line (HOS) ex...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::Although chemotherapy is initially effective in debulking tumor mass in a number of different types of malignancy, tumor cells gradually acquire chemoresistance and frequently progress to advanced clinical stage. Accumulating evidence has indicated that the tumor sensitivity to several chemotherapeutic drugs is regula...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7748
更新日期:2020-11-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most prevalent types of cancer worldwide and a common cause of morbidity and mortality in humans. The garlic-derived organosulfur compound diallyl trisulfide (DATS) has been shown to induce apoptosis in many human cancer cell lines in vitro and ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1882
更新日期:2012-09-01 00:00:00
abstract::MicroRNAs (miRNAs) play an important role in the genesis and development of gastric cancer. In the present study, we determined whether miRNA-135a-5p expression was increased in gastric cancer compared with adjacent non-tumor tissues using 20 pairs of gastric cancer and para-carcinoma tissue samples which were assesse...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3546
更新日期:2014-12-01 00:00:00