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Structure and assembly of turnip crinkle virus. VI. Identification of coat protein binding sites on the RNA.

Abstract：

:Structural studies of turnip crinkle virus have been extended to include the identification of high-affinity coat protein binding sites on the RNA genome. Virus was dissociated at elevated pH and ionic strength, and a ribonucleoprotein complex (rp-complex) was isolated by chromatography on Sephacryl S-200. Genomic RNA fragments in the rp-complex, resistant to RNase A and RNase T1 digestion and associated with tightly bound coat protein subunits, were isolated using coat-protein-specific antibodies. The identity of the protected fragments was determined by direct RNA sequencing. These approaches allowed us to study the specific RNA-protein interactions in the rp-complex obtained from dissociated virus particles. The location of one protected fragment downstream from the amber terminator codon in the first and largest of the three viral open reading frames suggests that the coat protein may play a role in the regulation of the expression of the polymerase gene. We have also identified an additional cluster of T1-protected fragments in the region of the coat protein gene that may represent further high-affinity sites involved in assembly recognition.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Wei N,Heaton LA,Morris TJ,Harrison SC

doi

10.1016/0022-2836(90)90148-F

subject

Has Abstract

pub_date

1990-07-05 00:00:00

pages

85-95

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(90)90148-F

journal_volume

214

pub_type

杂志文章

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