Abstract:
:Autophagy is an evolutionarily conserved, multi-step lysosomal degradation process in which a cell degrades its own long-lived proteins and damaged organelles. Ataxia telangiectasia mutated (ATM) has recently been shown to upregulate the process of autophagy. Previous studies showed that certain microRNAs, including miR-18a, potentially regulate ATM in cancer cells. However, the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells. In the present study, we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells. To determine whether a preliminary link exists between autophagy and miR-18a, HCT116 cells were irradiated and quantitative (q) PCR was performed to measure miR-18a expression. HCT116 cells were transfected with an miR-18a mimic to study its impact on indicators of autophagy. Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells. The results showed that miR-18a expression was strongly stimulated by radiation. Ectopic overexpression of miR-18a in HCT116 cell lines potently enhanced autophagy and ionizing radiation-induced autophagy. Moreover, miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity. Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development, progression, treatment and in other diseases.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Qased AB,Yi H,Liang N,Ma S,Qiao S,Liu Xdoi
10.3892/mmr.2012.1214subject
Has Abstractpub_date
2013-02-01 00:00:00pages
559-64issue
2eissn
1791-2997issn
1791-3004journal_volume
7pub_type
杂志文章abstract::The proliferation and differentiation of cardiac fibroblasts (CFs) is central to cardiac fibrosis. Betulinic acid (BA) is an active compound isolated from the bark of the birch tree Betula spp. (Betulaceae) and has been shown to attenuate hepatic fibrosis. However, the effect of BA on the high glucose‑induced fibrosis...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7323
更新日期:2017-11-01 00:00:00
abstract::Tumor necrosis factor α-induced protein 8 (TNFAIP8) has been associated with the tumorigenicity of various types of cancer, however, the expression of TNFAIP8 and its function in gastric cancer remain to be fully elucidated. Therefore, the present study examined the expression and biological function of TNFAIP8 in gas...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.3690
更新日期:2015-08-01 00:00:00
abstract::The production of reactive oxygen species (ROS) is a common phenomenon in podocyte impairment, which leads to the irreversible progression of chronic kidney diseases, such as diabetic kidney disease (DKD). Previous research has indicated that peroxisome proliferator‑activated receptor γ (PPARγ) coactivator‑1α (PGC‑1α)...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.8433
更新日期:2018-03-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7065
更新日期:2017-10-01 00:00:00
abstract::Non‑alcoholic fatty liver disease (NAFLD) is the most common fatty liver disease in developed countries, in which fat accumulation in the liver is induced by non‑alcoholic factors. The present study was conducted to identify NAFLD‑associated long non‑coding RNAs (lncRNAs), mRNAs and microRNAs (miRNAs). The microarray ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.9931
更新日期:2019-04-01 00:00:00
abstract::Colon cancer is characterized by invasion and migration. DNA methylation of CpG islands in tumor suppressor genes is considered to be an epigenetic mechanism underlying cancer development. Epigenetic silencing of a gene may be reversed by drugs, including genistein. The present study aimed to determine the effect of g...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.8760
更新日期:2018-05-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9136
更新日期:2018-08-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2011.478
更新日期:2011-07-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.2074
更新日期:2014-06-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 临床试验,杂志文章
doi:10.3892/mmr.2012.783
更新日期:2012-05-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.771
更新日期:2012-04-01 00:00:00
abstract::We wish to retract our article entitled 'Clinical, pathological and genetic characteristics of autosomal dominant inherited dynamin 2 centronuclear myopathy' published in Molecular Medicine Reports 13: 4273-4278, 2016. The article was submitted by the first author, Xinhong Liu, without the prior knowledge of the corre...
journal_title:Molecular medicine reports
pub_type: 杂志文章,撤回出版物
doi:10.3892/mmr.2016.5267
更新日期:2016-07-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6220
更新日期:2017-04-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.903
更新日期:2012-08-01 00:00:00
abstract::Adipocytes have been demonstrated to promote the progression of various tumors through modulation of cancer cell metabolism. However, their role in lung cancer progression remains undetermined. In the present study, adipocytes and lung adenocarcinoma A549 cells were cultured in a Transwell co‑culture system. Cancer ce...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9226
更新日期:2018-08-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.2355
更新日期:2014-09-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11485
更新日期:2020-11-01 00:00:00
abstract::Cryoinjury, or injury caused by extremely low temperatures, can occur in corneal endothelial cells (CECs) and lead to visual impairment. However, the mechanism of cryoinjury in CECs is not clear. The Stk11‑p53 signaling pathway regulates the proliferation and division of cells. Activity of the Stk11‑p53 signaling path...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4301
更新日期:2015-11-01 00:00:00
abstract::The tumor suppressor protein LKB1 is a serine/threonine kinase that plays a critical role in cell proliferation, and its inactivation has been linked to tumorigenesis in various cancer types. Current understanding of the LKB1 function is largely restricted to results from experiments on LKB1‑deficient cancer cells, wh...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.1916
更新日期:2014-03-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11108
更新日期:2020-07-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6165
更新日期:2017-04-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9624
更新日期:2019-01-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.8342
更新日期:2018-03-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.3583
更新日期:2015-08-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9041
更新日期:2018-07-01 00:00:00
abstract::The aim of the present study was to investigate the effect of hepatitis B virus X protein (HBx) on the expression of DNA methyltransferase (DNMT)3A/3B and suppressors of cytokine signaling‑1 (SOCS‑1), as well as promoter CpG island methylation of the SOCS‑1 gene. Stable hepatocyte cell lines expressing the HBx gene (p...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4545
更新日期:2016-01-01 00:00:00
abstract::Macrophage polarization serves an important role in immune regulation that is regulated by T‑cell immunoglobulin‑mucin‑3 (Tim‑3). The objective of the present study was to explore the role of 1,25‑dihydroxy‑vitamin D3 [1,25(OH)2D3] in macrophage polarization. Plasmid transfection techniques were applied to prepare RAW...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.10047
更新日期:2019-05-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11173
更新日期:2020-08-01 00:00:00
abstract::In the past decade, a group of cases with persisting haemocytopenia were separated from those with idiopathic cytopenia of undetermined significance due to the optimal response of these patients to immunosuppression therapy and due to the detection of autoantibodies in the bone marrow of haemopoietic cells. This condi...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.10663
更新日期:2019-11-01 00:00:00
abstract::Due to the rising abuse of ketamine usage in recent years, ketamine‑induced urinary tract syndrome has received increasing attention. The present study aimed to investigate the molecular mechanism underlying ketamine‑associated cystitis in a mouse model. Female C57BL/6 mice were randomly divided into two groups: One g...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.9907
更新日期:2019-04-01 00:00:00