Abstract:
STUDY DESIGN:We measured the expression and responses of Toll-Like Receptor 4 (TLR4) activation in the intervertebral disc (IVD) in vitro and in vivo. We hypothesize that stimulation of the IVD with the TLR4 ligand lipopolysaccharide (LPS) results in upregulation of a coordinated set of proinflammatory mediators and inhibition of matrix expression, both consistent with a molecular profile of degeneration. OBJECTIVE:To characterize early inflammatory and morphological changes induced by TLR4 activation in the IVD. SUMMARY OF BACKGROUND DATA:TLR4 is a pattern recognition receptor activated in innate immunity that has been implicated in disease mechanisms of inflammatory cartilaginous degeneration. However, no study to date has examined the expression and responses of TLR4 in the IVD. METHODS:IVD cells were stimulated with LPS in a dose-dependent manner, and inflammatory cytokine levels were measured by quantitative reverse transcription-polymerase chain reaction. Histological and inflammatory changes due to in vivo injection of LPS into the rat caudal IVD were measured by enzyme-linked immunosorbent assay and immunoblotting. RESULTS:Baseline TLR4 expression in IVD tissue varied according to cell type. LPS stimulation resulted in significant increases in tumor necrosis factor α (TNF)-α, interleukin (IL)-1β, IL-6, and nitric oxide levels and significant inhibition in aggrecan and collagen-2. Intradiscal injection of LPS was found to cause moderate degenerative changes in the IVD, with increases in tissue levels of IL-1β, TNF-α, high mobility group box 1 protein (HMGB1), and macrophage migration inhibitory factor (MIF). CONCLUSION:This study provides the first evidence that IVD cells express TLR4 and are responsive to TLR4 activation by upregulating a coordinated set of inflammatory cytokines. This study suggests that intradiscal injection of LPS offers a model for triggering inflammation of the IVD, demonstrating that inflammatory insults alone may potentially trigger degenerative changes of the IVD.
journal_name
Spine (Phila Pa 1976)journal_title
Spineauthors
Rajan NE,Bloom O,Maidhof R,Stetson N,Sherry B,Levine M,Chahine NOdoi
10.1097/BRS.0b013e31826b71f4subject
Has Abstractpub_date
2013-07-15 00:00:00pages
1343-51issue
16eissn
0362-2436issn
1528-1159journal_volume
38pub_type
杂志文章相关文献
SPINE文献大全abstract:STUDY DESIGN:This is a case report. OBJECTIVE:To report a case of soft-tissue reaction to wear debris and osteolysis around a pedicle screw after posterior spine fusion in a pediatric patient. SUMMARY OF BACKGROUND DATA:Posterior spine fusion with instrumentation is a standard procedure for stabilization and curve co...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3182793e51
更新日期:2013-01-01 00:00:00
abstract:STUDY DESIGN:Study of the diagnostic accuracy and interexaminer reliability of scoliosis diagnostic tests. OBJECTIVES:To estimate the sensitivity, specificity, and predictive value of the Scoliometer (National Scoliosis Foundation, Watertown, MA) and Adam's forward bend test in diagnosing scoliosis, and to determine t...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199804010-00011
更新日期:1998-04-01 00:00:00
abstract:STUDY DESIGN:Analysis of clinical database material collected prospectively. OBJECTIVE:Examination of the association between lateralization of scoliotic curves and the existence of underlying disease. SUMMARY OF BACKGROUND DATA:It has been suggested that left thoracic scoliosis configurations are intrinsically patho...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199906150-00010
更新日期:1999-06-15 00:00:00
abstract:STUDY DESIGN:Observational. OBJECTIVE:The authors present a detailed description of 25 skeletally immature patients with Chance fractures with a mean follow-up of 6.4 years. SUMMARY OF BACKGROUND DATA:Since the legislation mandating seat belt usage in Canada was first introduced, the fatality rate of automobile colli...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e31815a5986
更新日期:2007-11-15 00:00:00
abstract::Nineteen upper cervical spine specimens were dissected to examine the macroscopic and functional anatomy of alar ligaments. They are on both sides, symmetrically placed, approximately 10-13 mm long and elliptical in cross-section 3 X 6 mm in diameter. The fiber orientation is dependent on the height of dens axis, most...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-198703000-00016
更新日期:1987-03-01 00:00:00
abstract:STUDY DESIGN:Cost-utility analysis of an annular closure device (ACD) based on data from a prospective, multicenter randomized controlled trial (RCT) OBJECTIVE.: The aim of this study was to determine the cost-effectiveness of a novel ACD in a patient population at high risk for recurrent herniation following discectom...
journal_title:Spine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1097/BRS.0000000000002746
更新日期:2019-01-01 00:00:00
abstract:STUDY DESIGN:A study of mechanically induced herniation in punctured ovine discs followed by structural analysis. OBJECTIVE:To investigate whether an annular puncture influences the path that herniation takes by providing direct passage for nucleus through the annulus and therefore whether it increases the risk of acu...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000002336
更新日期:2018-04-01 00:00:00
abstract:STUDY DESIGN:Patients with cervicobrachial pain were examined in detail with special attention to vertebral artery loop formation. OBJECTIVES:To determine the incidence, short-time natural course and response to a conservative approach to vertebral artery loop formation in a group of patients with cervicobrachial pain...
journal_title:Spine
pub_type: 临床试验,杂志文章
doi:10.1097/01.BRS.0000067275.08517.58
更新日期:2003-06-01 00:00:00
abstract::The evaluation of the clinical utility of the clinical diagnostic tests and procedures, what works in spinal disorders, and for whom and how treatment affects a patient's symptoms and function are key questions of outcomes research. This paper describes the advantages and limitations of the main study approaches used....
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199409151-00004
更新日期:1994-09-15 00:00:00
abstract:STUDY DESIGN:Using a rabbit model of postnatal pulmonary hypoplasia, we investigated how expansion thoracoplasty affected growth of the spine and lungs. OBJECTIVE:By constricting the hemithorax in a growing rabbit to create postnatal pulmonary hypoplasia, we quantified how expansion thoracoplasty affects lung volume, ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181c4b8c7
更新日期:2010-01-15 00:00:00
abstract:STUDY DESIGN:A prospective study was conducted in patients who underwent surgeries for sciatica. The results were compared to those of control subjects. OBJECTIVES:This study expanded knowledge about the pathogenesis of back pain and sciatica in disc herniation. METHODS:Nucleus pulposus, retrieved from 10 patients wh...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199406000-00003
更新日期:1994-06-15 00:00:00
abstract::The outcome of spinal cord injury depends on the extent of secondary damage produced by a series of cellular and molecular events initiated by the primary trauma. This article reviews the evidence that secondary spinal cord injury involves the apoptotic as well as necrotic death of neurons and glial cells. Also discus...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/00007632-200007150-00022
更新日期:2000-07-15 00:00:00
abstract:STUDY DESIGN:Retrospective study of patients who underwent ventral discectomy and polymethyl-methacrylate (PMMA) interbody fusion for cervical radiculopathy. OBJECTIVES:To evaluate the long-term outcome after ventral discectomy and PMMA interbody fusion and to compare it with previous data from other surgical techniqu...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-200102010-00009
更新日期:2001-02-01 00:00:00
abstract:STUDY DESIGN:A retrospective analysis. OBJECTIVE:To evaluate the long-term clinical and radiographic outcomes and to investigate who achieved the successful outcomes after lumbar total disc replacement (TDR) using ProDisc II. SUMMARY OF BACKGROUND DATA:There are few evidences regarding the long-term efficacy and safe...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000001527
更新日期:2016-06-01 00:00:00
abstract:STUDY DESIGN:Retrospective multicenter database review. OBJECTIVE:The aim of this study was to evaluate national postoperative outcomes and hospital characteristics trends from 2001 to 2010 for advanced age CSM patients. SUMMARY OF BACKGROUND DATA:Recent studies show increases in US cervical spine surgeries and CSM d...
journal_title:Spine
pub_type: 杂志文章,多中心研究
doi:10.1097/BRS.0000000000001181
更新日期:2016-02-01 00:00:00
abstract::This is a retrospective review of 26 patients with chronic intractable pain in which dorsal column stimulation was used as a salvage procedure. On follow-up of 12 to 42.7 months, 21 of the patients had diminished narcotic usage. Seventeen patients subjectively rated their pain relief as good to excellent. Another five...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-198901000-00001
更新日期:1989-01-01 00:00:00
abstract:STUDY DESIGN:Retrospective study of instrumentation failure after posterior vertebral column resection (pVCR) in adult spinal deformity (ASD) patients. OBJECTIVE:The morbidity and related risk factors of the instrumentation failure. SUMMARY OF BACKGROUND DATA:Instrumentation failure is another common complication aft...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000001844
更新日期:2017-04-01 00:00:00
abstract:STUDY DESIGN:We performed a retrospective study of 668 consecutive patients with congenital spine deformities. OBJECTIVES:To determine the prevalence of Goldenhar conditions in patients with congenital spine deformities, and describe the types of vertebral abnormalities and their treatment. SUMMARY OF BACKGROUND DATA...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000219876.45640.2a
更新日期:2006-06-01 00:00:00
abstract::A young patient 13 years of age with traumatic sacrolisthesis at S1-S2 accompanied by neurologic damage was initially treated by traction to reduce the S1 dislocation over S2, and later by posterior arthrodesis using the technique of Luque. The procedure resulted in good stability and the patient showed marked orthope...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/00007632-199305000-00017
更新日期:1993-05-01 00:00:00
abstract:STUDY DESIGN:To identify mesenchymal stromal cells (MSC) from degenerate human nucleus pulposus (NP) and compare them with bone marrow (BM) MSC. OBJECTIVE:To test whether MSC obtained from NP and BM from the same subjects share similar biologic characteristics. SUMMARY OF BACKGROUND DATA:Recent studies have proposed ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181cb8828
更新日期:2010-12-15 00:00:00
abstract:STUDY DESIGN:Systematic review and meta-analysis. OBJECTIVE:To compare the treatment effects of observational studies versus randomized controlled trials (RCTs) in cervical disc arthroplasty. SUMMARY OF BACKGROUND DATA:RCTs can be logistically challenging and sometimes insufficiently generalizable; well-designed obse...
journal_title:Spine
pub_type: 杂志文章,meta分析,评审
doi:10.1097/BRS.0000000000001377
更新日期:2016-03-01 00:00:00
abstract:STUDY DESIGN:To determine the effect of cage/spacer stiffness on the stresses in the bone graft and cage subsidence. OBJECTIVE:To investigate the effect of cage stiffness on the biomechanics of the fused segment in the lumbar region using finite element analysis. SUMMARY OF BACKGROUND DATA:There are a wide variety of...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000250177.84168.ba
更新日期:2006-12-15 00:00:00
abstract:STUDY DESIGN:Experimental animal study. OBJECTIVE:To determine whether corticosteroids produce additional benefit to nerve root infiltration (NRI) for experimental lumbar disc herniation. SUMMARY OF BACKGROUND DATA:NRI is used for nonsurgical treatment of radicular symptoms caused by lumbar disc herniation or lumbar ...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181696132
更新日期:2008-04-01 00:00:00
abstract:STUDY DESIGN:Literature-based review. OBJECTIVE:To review the published data on occupant kinematic and neuromuscular responses during low-speed impacts and analyze how these data inform our understanding of whiplash injury. SUMMARY OF BACKGROUND DATA:A stereotypical kinematic and neuromuscular response has been obser...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3182387d71
更新日期:2011-12-01 00:00:00
abstract:STUDY DESIGN:Literature review of basic scientific and clinical research in spinal cord injury (SCI). OBJECTIVE:To provide physicians with an overview of the neurobiologic challenges of SCI, the current status of investigation for novel therapies that have been translated to human clinical trials, and the preclinical,...
journal_title:Spine
pub_type: 杂志文章,评审
doi:10.1097/BRS.0b013e3181f3286d
更新日期:2010-10-01 00:00:00
abstract:STUDY DESIGN:This study investigated the effect of neck muscle precontraction in aware occupants in whiplash. Head angulation relative to T1 and facet joint capsular ligament distractions were compared between aware and unaware occupants. OBJECTIVE:To quantify changes in facet joint capsular ligament distractions betw...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/01.brs.0000240210.23617.e7
更新日期:2006-09-15 00:00:00
abstract:STUDY DESIGN:Retrospective matched cohort. OBJECTIVE:To investigate the factors that may affect the decision-making process of adult deformity patients. SUMMARY OF BACKGROUND DATA:Adult deformity is a significant cause of morbidity in the elderly population. Despite high complication rates a significant number of pat...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0b013e3181851ba6
更新日期:2009-04-15 00:00:00
abstract:STUDY DESIGN:A cross-sectional study. OBJECTIVE:The aim of this study was to investigate the prevalence of ossification of posterior longitudinal ligament (OPLL) in patients with degenerative cervical myelopathy (DCM). SUMMARY OF BACKGROUND DATA:OPLL of the cervical spine is one of the main entities of DCM in Asian p...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000003526
更新日期:2020-10-01 00:00:00
abstract::MINI: Circulating microRNAs provide an insight into current disease states. Comparing patients with degenerative disc disease to healthy controls, patients with disc disease were found to have significantly downregulated levels of miR-155-5p. This marker was found to be an accurate diagnostic predictor for the presenc...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000003322
更新日期:2020-05-01 00:00:00
abstract:STUDY DESIGN:Retrospective database analysis. OBJECTIVE:To evaluate outcomes of human immunodeficiency virus (HIV) positive patients after cervical spine surgery. SUMMARY OF BACKGROUND DATA:Highly active antiretroviral medications have qualitatively altered the natural history of HIV, thus increasing the number of HI...
journal_title:Spine
pub_type: 杂志文章
doi:10.1097/BRS.0000000000001103
更新日期:2015-11-01 00:00:00