Abstract：
BACKGROUND:The role of DNA ploidy in genomic instability of cancer cells and prognosis has been described in a number of studies. The role of the centrosome in cell cycle has also been reported. AIM:In this study, we aimed to investigate the correlation between the centrosome and DNA ploidy in breast cancer in a search for a cytologic predictive and prognostic marker. MATERIALS AND METHODS:Cell prints were prepared from cell culture of mesothelial cells, fibroblast cell line MRC5 and breast cancer cell lines MCF7 and T47D. Indirect immunofluorescence was used with anti-γ-tubulin and centrosomes were quantified using a fluorescence microscope. DNA ploidy was scored with the DNA index analyzed by flow cytometry. RESULTS:The normal mesothelial cells (94% of the cells with one detected centrosome) and MRC5 diploid cells (68% with two centrosomes) were used as quality controls. A correlation between the number of centrosomes and DNA ploidy was found in MCF7 cell lines (64% of the cells with a number of centrosomes ≥ 3). It was not observed in invasive breast cancer samples; however, the frequency of cells with centrosomes ≥ 3 was found to be slightly higher in DNA aneuploid samples than in DNA diploid samples (15% vs 13.3%). CONCLUSION:Quantification of centrosome appears to be correlated to DNA ploidy in breast cancer cell lines and slightly associated to DNA aneuploidy in invasive breast cancer. Studies analyzing a larger number of samples as well as morphological abnormalities of the centrosome are needed.
journal_name
J Cytoljournal_title
Journal of cytologyauthors
Sakr RA,Fleury J,Prengel C,Bernaudin JF,Uzan S,Rouzier R,Darai Edoi
10.4103/0970-9371.97150subject
Has Abstractpub_date
2012-04-01 00:00:00pages
111-5issue
2eissn
0970-9371issn
0974-5165pii
JCytol-29-111journal_volume
29pub_type
杂志文章abstract：Introduction:The mortality due to uterine cervical cancer has been gradually increasing in women under 40 years of age (U40) in Japan. We investigated the effect of high-risk human papillomavirus (HR-HPV) on U40 subjects without any overt cytological abnormalities. Materials and Methods:We retrospectively examined the...
journal_title：Journal of cytology
pub_type： 杂志文章
doi：10.4103/JOC.JOC_148_17
更新日期：2019-07-01 00:00:00
abstract：:Histoplasmosis is a mycotic infection caused by dimorphic fungus, Histoplasma capsulatum. The organisms are usually found within the cells (macrophages). This organism mostly affects lungs in immunocompetent individuals and disseminated forms are seen in immunocompromised cases. Here, we describe a case of disseminate...
journal_title：Journal of cytology
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doi：10.4103/JOC.JOC_74_16
更新日期：2017-07-01 00:00:00
abstract：:Chondroid syringoma is a rare benign adnexal tumor of sweat glands with microscopic resemblance to the salivary gland pleomorphic adenoma. Cytology is rarely utilized for preoperative assessment of these slow-growing, small, nodular lesions. Definitive cytological diagnosis is also quite difficult, and majority of the...
journal_title：Journal of cytology
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doi：10.4103/0970-9371.145659
更新日期：2014-07-01 00:00:00
abstract：:Malignant mesothelioma of pleura is a locally aggressive rare neoplasm of mesothelial cells, which produces distant metastasis in advanced stages of its course. It is exceptional for patients to present with metastasis prior to the detection of primary tumor in the pleura. Here, we report a case of malignant mesotheli...
journal_title：Journal of cytology
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doi：10.4103/0970-9371.216123
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND:Image-guided fine-needle aspiration cytology (FNAC) serves as first-line diagnostic modalities for the assessment of mural thickening of the gallbladder (GB). AIM:The main objective of the present study was to correlate the diagnostic accuracy of preoperative image-guided FNAC of the thickened GB wall based...
journal_title：Journal of cytology
pub_type： 杂志文章
doi：10.4103/0970-9371.171231
更新日期：2015-10-01 00:00:00
abstract：:Herpes simplex virus (HSV) is a rare cause of breast infection. Few cases of maternal-infant transmission of the virus during breastfeeding resulting in nipple lesions have been documented. Only three cases have been reported in nonlactating women. We report an additional case of HSV mastitis in a 36-year-old nonlacta...
journal_title：Journal of cytology
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abstract：Background:Human papilloma virus (HPV) infection is the major etiologic agent of cervical carcinoma. The aim of this study was to determine the prevalence of HPV infection and genotype distribution in cervical swabs from 2,234 Turkish and 357 Albanian women with similar lifestyles from two different countries. Materia...
journal_title：Journal of cytology
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abstract：:The incidence of primary papillary carcinoma arising in thyroglossal cyst is rare and occurs in <1% of thyroglossal cysts. Even rarer is its diagnosis by pre-operative fine needle aspiration cytology (FNAC). Only 15 such cases diagnosed by FNAC have been previously reported in the literature. In this article, cytomorp...
journal_title：Journal of cytology
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更新日期：2010-01-01 00:00:00
abstract：:[This corrects the article on p. 153 in vol. 32, PMID: 26729974.]. ...
journal_title：Journal of cytology
pub_type： 已发布勘误
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更新日期：2016-01-01 00:00:00
abstract：Context:Intraoperative pathological assessment provides valuable information in a patient's diagnosis and management. Touch imprint/crush cytology is simple, rapid, and cost-effective. Also, imprint smears give excellent cytomorphology. Aims:To assess the utility and feasibility of intraoperative cytology technique as...
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pub_type： 杂志文章
doi：10.4103/JOC.JOC_94_19
更新日期：2020-07-01 00:00:00
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journal_title：Journal of cytology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:It is now known that the disease process of diabetes has effects on various tissues of the body. The following study was done to analyze the effects of diabetes on oral tissues. AIMS:To study the morphology and cytomorphometry of the cells obtained in cytologic smears from the buccal mucosa of diabetic pati...
journal_title：Journal of cytology
pub_type： 杂志文章
doi：10.4103/0970-9371.73291
更新日期：2010-10-01 00:00:00
abstract：:Synovial sarcomas are rare tumors accounting for approximately 5-10% of soft tissue sarcomas. They occur predominantly in the extremities, followed by head and neck. Primary pulmonary sarcomas are very rare and comprise only 0.5% of all primary lung malignancies. The diagnosis is established only after sarcomas like p...
journal_title：Journal of cytology
pub_type：
doi：10.4103/0970-9371.155240
更新日期：2015-01-01 00:00:00
abstract：:Prostatic adenocarcinoma (PA) is a common visceral malignancy of elderly men. Cutaneous metastasis of PA is rare. The incidence is <1%. A 55-year-old man presented with urinary symptoms and multiple cutaneous nodules around suprapubic region, inner aspect of both thighs and scrotum. Fine-needle aspiration cytology (FN...
journal_title：Journal of cytology
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doi：10.4103/0970-9371.160568
更新日期：2015-04-01 00:00:00
abstract：Aims:Tzanck smear is an old but useful test for diagnosis of cutaneous dermatoses. The aim of this study was to highlight the potential usefulness and diagnostic pitfalls of Tzanck smear for diagnosis of cutaneous dermatoses and infections. Materials and Methods:This hospital based cross-sectional study was carried ou...
journal_title：Journal of cytology
pub_type： 杂志文章
doi：10.4103/JOC.JOC_88_16
更新日期：2017-10-01 00:00:00
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journal_title：Journal of cytology
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doi：10.4103/0970-9371.151137
更新日期：2014-10-01 00:00:00
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journal_title：Journal of cytology
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doi：10.4103/0970-9371.73306
更新日期：2010-10-01 00:00:00
abstract：INTRODUCTION:Cervical intraepithelial neoplasia and cervical cancer remain important health problems. Cervical cytology by Papanicolaou (Pap) smears is an effective means of screening for cervical premalignant and malignant conditions. AIM:The aim of this study was to assess the prevalence of cervical dysplasia in pre...
journal_title：Journal of cytology
pub_type： 杂志文章
doi：10.4103/0970-9371.126659
更新日期：2013-10-01 00:00:00
abstract：:Tuberculosis of cervix is a rare disease. Tuberculosis usually affects women of childbearing age, indicating hormone dependence of infection. The patient presents with menstrual irregularities, infertility or vaginal discharge. Cervical lesions presents as papillary/vegetative growth or ulceration mimicking cervical c...
journal_title：Journal of cytology
pub_type：
doi：10.4103/0970-9371.93242
更新日期：2012-01-01 00:00:00
abstract：:Hepatoblastoma (HBL) is a rare primary malignant liver tumor affecting mainly pediatric patients in the age group 6 months to 3 years. Presentation of HBL in the neonatal period is rare. HBL can be diagnosed on cytology along with subtyping. Estimation of serum alpha-fetoprotein (AFP) is essential as a tumor marker. F...
journal_title：Journal of cytology
pub_type：
doi：10.4103/0970-9371.103945
更新日期：2012-10-01 00:00:00
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journal_title：Journal of cytology
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更新日期：2019-04-01 00:00:00
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journal_title：Journal of cytology
pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
abstract：:Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease) is a rare benign disease of unknown etiology presenting with massive lymphadenopathy. These cases can often be misdiagnosed as lymphoma. Hence, it is important to distinguish Rosai-Dorfman disease from lymphoma and other causes of histiocytosis b...
journal_title：Journal of cytology
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doi：10.4103/0970-9371.55229
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND:The use of oral exfoliative cytology as a diagnostic aid accentuates the need for establishing an accurate baseline, thereby enabling the comparison of abnormal oral tissue with established baseline. AIMS AND OBJECTIVE:To detect any changes in the nuclear area (NA), cytoplasmic area (CA), and nuclear:cytopl...
journal_title：Journal of cytology
pub_type： 杂志文章
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更新日期：2011-04-01 00:00:00
abstract：:Primary splenic angiosarcoma is a rare malignant vascular neoplasm of mesenchymal origin. The tumor is highly aggressive and has a high metastatic potential. It is usually diagnosed on histopathological examination of splenectomy specimen. Only few cases of angiosarcoma diagnosed by fine-needle aspiration (FNA) cytolo...
journal_title：Journal of cytology
pub_type：
doi：10.4103/JOC.JOC_148_16
更新日期：2018-04-01 00:00:00
abstract：:Salivary gland small cell carcinoma (SGSmCC) is extremely rare, accounting for less than 1% of salivary gland tumors. A 42-year-old female patient came to the outpatient clinic due to a mass localized in the left submandibular space and that had been growing since 3-4 months. The fine needle aspiration cytology (FNAC)...
journal_title：Journal of cytology
pub_type：
doi：10.4103/0970-9371.175509
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Fine needle aspiration cytology (FNAC) of oral and maxillofacial region has not been widely utilized for diagnosis due to diversity of lesion types, heterogeneity of cell populations and difficulties in reaching and aspirating these lesions. AIM:Our aim was to demonstrate the effectiveness of this cheap and...
journal_title：Journal of cytology
pub_type： 杂志文章
doi：10.4103/0970-9371.83461
更新日期：2011-07-01 00:00:00
abstract：:Primary (spontaneous) umbilical endometriosis is very rare with an estimated incidence of 0.5-1% of all patients with endometrial ectopia. Endometriosis is a common gynecological condition, the pelvis being the most common site of the disease. Extrapelvic site is less common and even more difficult to diagnose due to ...
journal_title：Journal of cytology
pub_type：
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更新日期：2011-10-01 00:00:00
abstract：:Fibromatosis colli is a peculiar, benign fibrous growth of the sternocleidomastoid that usually appears during the first few weeks of life and is often associated with muscular torticollis. Fibromatosis colli (FC) is seen in children born after difficult, prolonged labor, assisted delivery, and breech deliveries. Clin...
journal_title：Journal of cytology
pub_type：
doi：10.4103/0970-9371.54869
更新日期：2009-01-01 00:00:00
