Abstract:
PURPOSE:Osteoporosis mainly involves cancellous bone, and the spine and hip, with their relatively high cancellous bone to cortical bone ratio, are severely affected. Studies of bone mesenchymal stem cells (BMSCs) from osteoporotic patients and animal models have revealed that osteoporosis is often associated with reduction of BMSCs' proliferation and osteogenic differentiation. Our aim was to test whether polylactic acid-polyglycolic acid copolymer(PLGA)/collagen type I(CoI) microspheres combined with BMSCs could be used as injectable scaffolds to improve bone quality in osteoporotic female rats. METHODS:PLGA microspheres were coated with CoI. BMSCs of the third passage and were cultured with PLGA/CoI microspheres for seven days. Forty three-month-old female non-pregnant SD rats were ovariectomized to establish osteoporotic animal models. Three months after being ovariectomized, the osteoporotic rats were randomly divided into five groups: SHAM group, PBS group, cell group, microsphere (MS) group, and cell+MS group. Varying materials were injected into the intertrochanters of each group's rats. Twenty rats were sacrificed at one month and three months post-op, respectively. The femora were harvested in order to measure the intertrochanteric bone mineral density (BMD) with DEXA and trabecular thickness (Tb.Th), percentage of trabecular area (%Tb.Ar), bone volume fraction (BV/TV) and trabecular spacing (Tb.Sp) with Micro CT. One-way ANOVA and Kruskal-Wallis tests were used. RESULTS:BMSCs seeded on PLGA/CoI microspheres had a nice adhesion and proliferation. At one month post-op, the BMD (0.33 ± 0.01 g/cm(2)), Tb.Th (459.65 ± 28.31 μm), %Tb.Ar (9.61 ± 0.29 %) and Tb.Sp (2645.81 ± 94.91 μm) of the cell+ MS group were better than those of the SHAM group and the cell group. At three months post-op, the BMD (0.32 ± 0.01 g/cm(2)), Tb.Th (372.81 ± 38.45 μm), %Tb.Ar (6.65 ± 0.25 %), BV/TV (6.62 ± 0.25 %) and Tb.Sp (1559.03 ± 57.06 μm) of the cell + MS group were also better than those of the SHAM group and the cell group. CONCLUSION:The PLGA/CoI microspheres combined with BMSCs can repair bone defects more quickly. This means that PLGA/CoI microspheres combined with BMSCs can promote trabecular reconstruction and improve bone quality in osteoporotic rats. This scaffold can provide a promising minimally invasive surgical tool for enhancement of bone fracture healing or prevention of fracture occurrence which will in turn minimize complications endemic to patients with osteoporosis.
journal_name
Int Orthopjournal_title
International orthopaedicsauthors
Yu Z,Zhu T,Li C,Shi X,Liu X,Yang X,Sun Hdoi
10.1007/s00264-012-1543-4subject
Has Abstractpub_date
2012-10-01 00:00:00pages
2163-71issue
10eissn
0341-2695issn
1432-5195journal_volume
36pub_type
杂志文章abstract:PURPOSE:The increase in methicillin-resistant Staphylococcus aureus (MRSA) infections is currently a major health care problem. Vancomycin is still often the first-line anti-microbiological agent for treating such infections; however, a recent decline in efficacy of vancomycin in MRSA infections has raised concerns and...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-2149-1
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:Short-stem hip arthroplasty preserves femoral bone stock which includes the femoral neck. This implies that the stem has to follow the anatomy of the femoral neck. Therefore, it has been questioned whether biomechanical reconstruction of the hip can be safely achieved with SHA. METHODS:Biomechanical reconstruc...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-011-1477-2
更新日期:2012-07-01 00:00:00
abstract::We report on two cases of patients in whom hypocalcemic seizures during hemodialysis led to right scapular body fracture in one and bilateral femoral neck fractures in the other. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640050377
更新日期:1999-01-01 00:00:00
abstract::Between 1987 and 1991 the spine was stabilised in 205 patients using corundum ceramic implants. The radiological results were assessed with a follow up of from 6 to 24 months and were graded as good in 81%. Poor results were not due to the implanted material, but were usually caused by failure to obtain full correctio...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00180170
更新日期:1994-02-01 00:00:00
abstract::An osteosarcoma developed in a boy, 16 years of age, 9 years after staples had been inserted in the region of the lower femoral epiphysis. The possible association between the implant and the tumour is discussed. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640050090
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:The objective of this report was to analyse injury patterns and definitive management of local casualties with multiple blast extremity injuries in the Kabul International Airport Combat Support Hospital. METHODS:A clinical prospective study was performed from July 2012 to January 2013. Afghan victims of a bla...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2532-6
更新日期:2014-12-01 00:00:00
abstract::Over a 4-year period, 55 consecutive knee arthroscopies were performed on 54 patients with symptomatic mild to moderate osteoarthritis. There were 30 female patients and the average patient age was 60.9 years. The average follow-up period was 29.6 months. All patients underwent diagnostic arthroscopy and washout. Furt...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640000212
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:The purpose of the study was to evaluate the treatment of five patients with proximal tibial epiphysis preserving resection for malignant tumours of the tibia. METHOD:Three patients suffered from Ewing sarcoma, two had osteosarcoma. The proximal level of the resection was in the line of the growth plate in fou...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-012-1731-2
更新日期:2013-01-01 00:00:00
abstract::A man of 71 was seen with a solitary metastasis in the patella as the first presentation of carcinoma of the lung. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00266728
更新日期:1989-01-01 00:00:00
abstract::Thirty-two patients, who had frozen shoulders which had not improved with physiotherapy, were treated by manipulation under general anaesthesia or by steroid injection and hydraulic distension under local anaesthesia. Distension is recommended as it is easy to carry out and gave better results than manipulation. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00181697
更新日期:1993-11-01 00:00:00
abstract::We describe a hemiarthroplasty of the shoulder with an acrylic Judet hip prosthesis, inserted after the excision of a giant cell tumour of the head of the humerus, which functioned well for 39 years. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::We conducted a prospective, randomised study on primary total knee replacements to evaluate the effects of tourniquet use on total calculated blood loss using Gross formula, post-operative measured blood loss, operating time, need for blood transfusion, post-operative pain, analgesia requirement and knee flexion. Fort...
journal_title:International orthopaedics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00264-002-0360-6
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:Surgical treatment of acetabular fractures with plate fixation is challenging for orthopaedic surgeons because of variations of the surface curvature and complex fracture patterns of the acetabulum. We present our experience with pre-operative computer-assisted virtual simulation and three-dimensional (3D) prin...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-018-4111-8
更新日期:2019-08-01 00:00:00
abstract:PURPOSE:To determine the most common injury patterns, root cause, and the frequency with which unrecognized contralateral posterior ring injury occurs in patients presenting with surgically treated pelvic fractures. METHODS:The medical records of 73 patients presenting to our level I trauma center (52 male and 21 fema...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-018-4268-1
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:From February 21, the day of hospitalisation in ICU of the first diagnosed case of Covid-19, the social situation and the hospitals' organisation throughout Italy dramatically changed. METHODS:The CIO (Club Italiano dell'Osteosintesi) is an Italian society devoted to the study of traumatology that counts me...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04637-3
更新日期:2020-08-01 00:00:00
abstract:PURPOSE:The goal of total hip endoprosthesis is to achieve painless and functional hip for long term. Accurate reconstruction of hip anatomy largely depends on the implant design. In order to select an implant in correspondence with the native hip, the proximal femoral morphology has been in focus of many studies in th...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-021-04942-5
更新日期:2021-01-13 00:00:00
abstract:PURPOSE:To analyse the results of the Ponseti method in the treatment of non-idiopathic clubfeet and compare them with idiopathic clubfeet in a programme-based setting using a standardized protocol METHODS: We reviewed two groups of 782 children treated prospectively in our programme-based Clubfoot Clinic: group I comp...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04677-9
更新日期:2021-01-01 00:00:00
abstract:INTRODUCTION:Lateral humeral condylar fractures are the second most common elbow fractures in children. We present the long-term clinical and radiographic results. MATERIAL AND METHODS:All children (<16 years) in the geographic catchment area with lateral condylar fracture were asked to participate and 32 (76.2%) pati...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-017-3451-0
更新日期:2017-07-01 00:00:00
abstract::The concentrations of cobalt and chromium have been measured in the urine of 22 patients who had received uncemented hip prostheses made from cobalt, chromium and molybdenum alloy, and in 21 patients who had not received prostheses, using absorption spectrophotometry. The results were statistically analysed using the ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00454409
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:Although there are various new scaffold-based techniques for cartilage regeneration it remains unclear up to which defect size they can be used. The present study reports of a cell-free collagen type I gel matrix for the treatment of large cartilage defects of the knee after a two-year follow-up. METHODS:Twent...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-015-2695-9
更新日期:2015-12-01 00:00:00
abstract::The aim of this prospective study was to determine the effect of screening for methicillin-resistant Staphylococcus aureus (MRSA), in patients undergoing total hip and knee replacements, on reducing hospital-acquired infections and the length of hospital stay. We included 395 patients admitted to the elective orthopae...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-005-0649-3
更新日期:2005-06-01 00:00:00
abstract:PURPOSE:Occult intra-operative periprosthetic acetabular fracture is a seldom-reported complication of primary total hip arthroplasty (THA). It may potentially be associated with cup instability and implant loosening. The present study aimed to investigate clinical consequences of this complication. METHODS:Between 20...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-018-4084-7
更新日期:2019-07-01 00:00:00
abstract::Developmental dysplasia of the hip (DDH) is a spectrum of disorders that results in anatomic abnormalities leading to increased contact stress in the joint and, eventually, secondary osteoarthritis. However, many patients with DDH become symptomatic before the severe degenerative changes of the hip because of abnormal...
journal_title:International orthopaedics
pub_type: 杂志文章,评审
doi:10.1007/s00264-015-2927-z
更新日期:2016-05-01 00:00:00
abstract:PURPOSE:The prevalence of joint hypermobility (JH) in patients with femoroacetabular impingement (FAI) and its association with outcomes is yet widely unknown. This study aimed to validate a self-reported version of the Beighton score for FAI patients, and to determine possible associations between JH and clinical and ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2424-9
更新日期:2014-11-01 00:00:00
abstract:PURPOSE:The aim of this study was to compare angiogenic cytokine levels in knee osteoarthritis (OA) patients and healthy controls and to investigate the relationships between angiogenic cytokines and the OA severity. METHODS:Thirty-one knee OA patients and 15 healthy controls were recruited. Nine angiogenic cytokines ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2406-y
更新日期:2014-09-01 00:00:00
abstract:PURPOSE:This study describes a case series of 15 patients with radial head fractures who underwent radial head excision using an arthroscopic technique. METHODS:Over a four year period, 15 patients (average age 49.6 years) who had sustained a radial head or radial neck fracture underwent an arthroscopic excision of th...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-012-1667-6
更新日期:2012-12-01 00:00:00
abstract::The haematological and orthopaedic complications of patients with sickle cell disease from two different regions of Arabia are presented. Nineteen patients from the South-western region were matched for age and sex with an equal number from the Eastern region. The mean sickle cell haemoglobin was 75.95% in those from ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00182718
更新日期:1992-01-01 00:00:00
abstract:PURPOSE:We prospectively compared two types of dressing (conventional gauze-based versus absorbing hydrofibre) after primary total hip (THA) or knee (TKA) arthroplasties. METHODS:Eighty candidates for THA (n = 40) or TKA (s = 40) were randomized: gauze-based versus hydrofibre absorbing (Aquacel®, ConvaTec). The two gr...
journal_title:International orthopaedics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00264-015-2726-6
更新日期:2015-07-01 00:00:00
abstract::We measured cytokine secretion patterns in peripheral blood and granulation tissue by flow cytometry in 16 human immunodeficiency virus (HIV) positive and 26 HIV negative patients with spinal tuberculosis. Anti-retroviral therapy was not prescribed. There were no significant differences in the postoperative morbidity ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640000125
更新日期:2000-01-01 00:00:00
abstract::Joint arthroplasty registries show an increased rate of aseptic loosening in uncemented acetabular components as compared to cemented acetabular components. Since loosening is associated with particulate wear debris, we postulated that uncemented acetabular components demonstrate a higher polyethylene wear rate than c...
journal_title:International orthopaedics
pub_type: 杂志文章,评审
doi:10.1007/s00264-012-1738-8
更新日期:2013-01-01 00:00:00