Quantitative retinal protein analysis after optic nerve transection reveals a neuroprotective role for hepatoma-derived growth factor on injured retinal ganglion cells.

abstract：PURPOSE:We examined if light induces changes in the retinal structure that can be observed using optical coherence tomography (OCT). METHODS:Normal C57BL/6J mice (age 3-6 months) adapted to either room light (15 minutes to ∼5 hours, 50-500 lux) or darkness (overnight) were imaged using a Bioptigen UHR-OCT system. Conf...

journal_title：Investigative ophthalmology & visual science

authors： Li Y,Fariss RN,Qian JW,Cohen ED,Qian H

更新日期：2016-07-01 00:00:00

abstract：PURPOSE:Previous in vitro studies with transgenic and gene-knockout mice have shown that lenses with elevated levels of glutathione peroxidase (GPX)-1 activity are able to resist the cytotoxic effect of H(2)O(2), compared with normal lenses and lenses from GPX-1-deficient animals. The purpose of this study was to inves...

journal_title：Investigative ophthalmology & visual science

authors： Reddy VN,Giblin FJ,Lin LR,Dang L,Unakar NJ,Musch DC,Boyle DL,Takemoto LJ,Ho YS,Knoernschild T,Juenemann A,Lütjen-Drecoll E

更新日期：2001-12-01 00:00:00

abstract：:The authors compared cytoskeletal elements of the in situ human trabecular-meshwork cell with in situ human corneal cells using indirect immunofluorescence staining for tubulin and intermediate filaments (vimentin, cytokeratin, and desmin) and NBD-phallacidin staining for f-actin using both fixed frozen and unfixed fr...

journal_title：Investigative ophthalmology & visual science

authors： Weinreb RN,Ryder MI

更新日期：1990-09-01 00:00:00

abstract：:Retinal dysplasia is a failure in normal retinal development. The morphologic sequence of the dysplastic processes was examined in fetuses and neonates from dogs affected with an inherited form of retinal dysplasia. The dysplastic change has its onset at 45-50 days' gestation and is most prominent in the dorsal peripa...

journal_title：Investigative ophthalmology & visual science

authors： Whiteley HE

更新日期：1991-04-01 00:00:00

abstract：:PURPOSE. To investigate the characteristics of a spectral-domain optical coherence tomography (SD-OCT) image phenomenon known as the mirror artifact, calculate its prevalence, analyze potential risk factors, measure severity, and correlate it to spherical equivalent and central visual acuity (VA). METHODS. OCT macular...

journal_title：Investigative ophthalmology & visual science

authors： Ho J,Castro DP,Castro LC,Chen Y,Liu J,Mattox C,Krishnan C,Fujimoto JG,Schuman JS,Duker JS

更新日期：2010-07-01 00:00:00

abstract：:Photoreceptors can segregate inner segment proteins from outer segment proteins. It is uncertain whether such a sorting process occurs during disc morphogenesis in rods, thereby resulting in an outer segment plasma membrane which differs compositionally from the discs. In this study, the plasma membranes of intact, pu...

journal_title：Investigative ophthalmology & visual science

authors： Polans AS,Burton MD

更新日期：1988-10-01 00:00:00

abstract：PURPOSE:To study possible causes of motility disturbances that may result from orbital decompression surgery in patients with Graves orbitopathy and especially the role of rectus extraocular muscle paths. METHODS:Sixteen patients with Graves orbitopathy were studied before and 3 to 6 months after translid (6 patients)...

journal_title：Investigative ophthalmology & visual science

authors： Abràmoff MD,Kalmann R,de Graaf ME,Stilma JS,Mourits MP

更新日期：2002-02-01 00:00:00

abstract：PURPOSE:It is well documented that grafting of cells in the subretinal space of Royal College of Surgeons (RCS) rats limits deterioration of vision and loss of photoreceptors if performed early in postnatal life. What is unclear is whether cells introduced later, when photoreceptor degeneration is already advanced, can...

journal_title：Investigative ophthalmology & visual science

authors： Wang S,Lu B,Girman S,Holmes T,Bischoff N,Lund RD

更新日期：2008-01-01 00:00:00

abstract：Purpose:Synthesis of keratan sulfate (KS) relies on coordinated action of multiple enzymes, including the N-acetylglucosamine-transferring enzyme, β-1,3-N-acetylglucosaminyltransferase-7 (β3GnT7). A mouse model deficient in β3GnT7 was developed to explore structural changes in KS and the extracellular matrix (ECM; i.e....

journal_title：Investigative ophthalmology & visual science

authors： Littlechild SL,Young RD,Caterson B,Yoshida H,Yamazaki M,Sakimura K,Quantock AJ,Akama TO

更新日期：2018-03-01 00:00:00

abstract：:Patients with choroidal melanomas and simulating choroidal tumors were tested in a masked manner in a leukocyte-migration inhibition (LMI) assay with a 3M KCl antigen derived from a metastatic choroidal melanoma. Good discrimination petween patient groups was observed with this antigen. The sensitivity and specificity...

journal_title：Investigative ophthalmology & visual science

authors： Char DH

更新日期：1977-02-01 00:00:00

abstract：Purpose:Our goal was to generate and characterize a new mouse model in which only angiogenesis- and glaucoma-relevant tissues would be naturally fluorescent. The Matrix Gla (MGP) gene is highly expressed in vascular smooth muscle cells (VSMC) and trabecular meshwork (TM). We sought to direct our Mgp-Cre.KI mouse recomb...

journal_title：Investigative ophthalmology & visual science

authors： Asokan P,Mitra RN,Periasamy R,Han Z,Borrás T

更新日期：2018-02-01 00:00:00

abstract：PURPOSE:The purpose of the present study was to investigate metabolic changes in different compartments of the rat lens (anterior, nuclear, posterior, and equatorial) after exposure to an acute double threshold dose of ultraviolet-B radiation (UVR-B) by using high-resolution magic angle spinning (HR-MAS) (1)H nuclear m...

journal_title：Investigative ophthalmology & visual science

authors： Tessem MB,Bathen TF,Löfgren S,Saether O,Mody V,Meyer L,Dong X,Söderberg PG,Midelfart A

更新日期：2006-12-01 00:00:00

abstract：:We use a population balance model to study the mechanism and the rate of centripetal migration of epithelial cells, renewal of the corneal epithelial population by the cell derived from (progeny of) the limbal stem cells and the kinetics of the replacement of the donor corneal epithelium. The epithelial mass is consta...

journal_title：Investigative ophthalmology & visual science

authors： Sharma A,Coles WH

更新日期：1989-09-01 00:00:00

abstract：PURPOSE:To investigate the role of the gap junction protein connexin43 (Cx43), which is predominantly expressed in lens epithelial cells in the control of lens development and organization. METHODS:Newborn mice in which the Cx43 gene was disrupted by homologous recombination were used. Lenses from Cx43 (-/-) mice and ...

journal_title：Investigative ophthalmology & visual science

authors： Gao Y,Spray DC

更新日期：1998-06-01 00:00:00

abstract：PURPOSE:Inhibitors of 3-hydroxy-3-methylglutaryl CoA reductase (statins) reduce signs of diabetic retinopathy in diabetic patients and animals. Indirect clinical evidence supports the actions of statins in improving cardiovascular function, but the mechanisms of their protective actions in the retina are not understood...

journal_title：Investigative ophthalmology & visual science

authors： Al-Shabrawey M,Bartoli M,El-Remessy AB,Ma G,Matragoon S,Lemtalsi T,Caldwell RW,Caldwell RB

更新日期：2008-07-01 00:00:00

abstract：PURPOSE:The aim of this study was to evaluate a DNA dot hybridization assay (DHA) for assessing bacterial bioburden in orthokeratology lens (OK) storage cases. METHODS:Forty-one OK wearers participated in this study. The dot hybridization assay was used to assess the bacterial bioburden of OK after removal and 6-hour ...

journal_title：Investigative ophthalmology & visual science

authors： Kuo MT,Chien CC,Lo J,Hsiao CC,Tseng SL,Lai YH,Fang PC,Chang TC

更新日期：2014-12-23 00:00:00

abstract：PURPOSE:To evaluate the longitudinal variability of optic disc and retinal nerve fiber layer (RNFL) measurements obtained from optical coherence tomography (OCT), scanning laser polarimetry (SLP), and confocal scanning laser ophthalmoscopy (CSLO). METHODS:Forty-five normal and 43 glaucomatous eyes of 88 subjects were ...

journal_title：Investigative ophthalmology & visual science

authors： Leung CK,Cheung CY,Lin D,Pang CP,Lam DS,Weinreb RN

更新日期：2008-11-01 00:00:00

abstract：PURPOSE:Focal opacities are signs of early cataractogenesis in the human lens. They progress slowly over a lifetime and may be precursors of mature cataracts. The authors analyzed changes in proteins, phospholipids, and cholesterol in these opacities using in situ techniques: Raman microspectroscopy, filipin cytochemis...

journal_title：Investigative ophthalmology & visual science

authors： Duindam JJ,Vrensen GF,Otto C,Greve J

更新日期：1998-01-01 00:00:00

abstract：Purpose:To investigate by optical coherence tomography angiography (OCT-A) the choroidal vascular response to experimentally increased blood pressure in patients with central serous chorioretinopathy (CSCR). Methods:For this multicenter, observational, case-control study, we enrolled 35 patients with an established di...

journal_title：Investigative ophthalmology & visual science

authors： Cardillo Piccolino F,Lupidi M,Cagini C,Fruttini D,Nicolò M,Eandi CM,Tito S

更新日期：2018-08-01 00:00:00

abstract：PURPOSE:To investigate feasibility and potential uses of flow cytometry in impression cytology as a new procedure to assess and quantify conjunctival inflammation. METHODS:Specimens for cytology were collected by impression from 30 patients with various chronic ocular surface disorders and from 10 normal subjects. Two...

journal_title：Investigative ophthalmology & visual science

authors： Baudouin C,Brignole F,Becquet F,Pisella PJ,Goguel A

更新日期：1997-06-01 00:00:00

abstract：PURPOSE:To evaluate the ocular complications of intravitreal injection of high dose bevacizumab in monkey eyes. METHODS:Four healthy monkeys (Macaca fuscata) received an intravitreal injection of either 6.25 or 12.5 mg bevacizumab in the right eye; the left control eye received an intravitreal injection of the same vo...

journal_title：Investigative ophthalmology & visual science

authors： Sakurai K,Akiyama H,Shimoda Y,Yoshida I,Kurabayashi M,Kishi S

更新日期：2009-10-01 00:00:00

abstract：PURPOSE:To report neural rim dimensions for South Indians and examine rim shape with relevance to clinical evaluation. METHODS:Healthy phakic participants (n = 623) of the Chennai Glaucoma Study with normal frequency-doubling perimetry underwent complete eye examinations including optic disc digital stereophotography....

journal_title：Investigative ophthalmology & visual science

authors： Arvind H,George R,Raju P,Ve RS,Mani B,Kannan P,Vijaya L

更新日期：2008-08-01 00:00:00

abstract：PURPOSE:We investigated the neuroprotective effect of human serum albumin nanoparticles (HSA-NPs) and their conjugation with brimonidine (HSA-Br-NPs) on retinal ganglion cells (RGCs) in optic nerve crush (ONC) model. METHODS:We fabricated HSA-Br-NPs by ethanol precipitation, including 0.18% brimonidine (Br) and 3.5% h...

journal_title：Investigative ophthalmology & visual science

authors： Kim KE,Jang I,Moon H,Kim YJ,Jeoung JW,Park KH,Kim H

更新日期：2015-08-01 00:00:00

abstract：PURPOSE:To characterize in SV40-immortalized human corneal epithelial cells (tHCEC) osmosensitive taurine transporter gene and protein expression as well as its functional activity. To evaluate whether medium supplementation with taurine improves cell viability during a hypertonic challenge. METHODS:tHCEC were preincu...

journal_title：Investigative ophthalmology & visual science

authors： Shioda R,Reinach PS,Hisatsune T,Miyamoto Y

更新日期：2002-09-01 00:00:00

abstract：PURPOSE:The objective of this study was to determine whether alteration in mucins could be detected in patients with dry eye symptoms by using the monoclonal antibody H185, which recognizes carbohydrate epitopes on mucin molecules. METHODS:Immunohistochemistry and immunoelectron microscopy was used to examine binding ...

journal_title：Investigative ophthalmology & visual science

authors： Danjo Y,Watanabe H,Tisdale AS,George M,Tsumura T,Abelson MB,Gipson IK

更新日期：1998-12-01 00:00:00

abstract：PURPOSE:To investigate morphological characteristics of optic nerve head and peripapillary region with myopia and glaucoma. METHODS:Ten normal and 17 glaucomatous myopic participants were imaged with a custom 1060-nm swept-source optical coherence tomography system. The three-dimensional images were processed and segm...

journal_title：Investigative ophthalmology & visual science

authors： Lee S,Han SX,Young M,Beg MF,Sarunic MV,Mackenzie PJ

更新日期：2014-06-03 00:00:00

abstract：PURPOSE:Retinal injury induces Müller cell dedifferentiation by activating extracellular signal-regulated kinase (ERK) signaling. Stimulation of α2-adrenergic receptors protects against injury but also activates ERK in Müller cells. The purpose of this work was to study the effect of α2-adrenergic signaling on injury-i...

journal_title：Investigative ophthalmology & visual science

authors： Harun-Or-Rashid M,Díaz-DelCastillo M,Galindo-Romero C,Hallböök F

更新日期：2015-09-01 00:00:00

abstract：PURPOSE:We assessed whether partial occlusion of the nonamblyopic eye with Bangerter filters can immediately reduce suppression and promote binocular summation of contrast in observers with amblyopia. METHODS:In Experiment 1, suppression was measured for 22 observers (mean age, 20 years; range, 14-32 years; 10 females...

journal_title：Investigative ophthalmology & visual science

authors： Chen Z,Li J,Thompson B,Deng D,Yuan J,Chan L,Hess RF,Yu M

更新日期：2014-10-28 00:00:00

abstract：PURPOSE:RPE cell activation is an important feature of autoimmune uveitis. This investigation focused on whether extracellular nucleotides could contribute to this activation, and the effects of ATPgammaS, UTP, and UDP on the production of IL-8 by RPE cells was studied in relation to their expression of functional P2Y ...

journal_title：Investigative ophthalmology & visual science

authors： Relvas LJ,Bouffioux C,Marcet B,Communi D,Makhoul M,Horckmans M,Blero D,Bruyns C,Caspers L,Boeynaems JM,Willermain F

更新日期：2009-03-01 00:00:00

abstract：PURPOSE:To determine whether the lipid peroxidation inhibitor tirilazad mesylate can block the death of retinal ganglion cells induced by the inhibition of oxidative phosphorylation and glycolysis. METHODS:Rat retinal ganglion cells were labeled retrogradely with the fluorescent tracer DiI, and mixed cultures were pre...

journal_title：Investigative ophthalmology & visual science

authors： Levin LA,Clark JA,Johns LK

更新日期：1996-12-01 00:00:00