Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:Artemisia princeps Pampanini is widely used in Eastern traditional medicine for the treatment of circulatory disorders, such as, dysmenorrhea, hematuria, hemorrhoids, and inflammation, and is also used to treat chronic conditions, such as, cancers, ulcers, and digestive disorders. AIM OF THE STUDY:The purpose of this study is to investigate the effect of a standardized flavonoid-rich fraction of Artemisia princeps Pampanini cv. Sajabal (FRAP) on the induction of apoptosis and the molecular mechanism involved in human cervical cancer HeLa cells. MATERIALS AND METHODS:Human cervical cancer HeLa cells were treated with FRAP and apoptosis was detected by cell morphologic observation, annexin-V-PI staning and western blot analysis on the expression of protein associated with cell death. RESULTS:FRAP led to the cleavages of caspase-3, -8, and -9 and the cleavage of poly (ADP-ribose) polymerase (PARP) in HeLa cells. Caspase-3 inhibitor (z-DEVD-fmk), caspase-8 inhibitor (z-IETD-fmk), caspase-9 inhibitor (z-LEHD), and broad caspase inhibitor (z-VAD-fmk) significantly suppressed the FRAP-induced accumulation of annexin V positive cells. Furthermore, it was found that FRAP caused a loss of mitochondrial membrane potential (MMP) and the release of cytochrome c to the cytosol. Furthermore, the overexpression of Bcl-xL significantly prevented FRAP-induced apoptosis, MMP changes, and the activations of caspase-3, -8, and -9. Interestingly, pretreatment with caspase-8 inhibitor significantly reduced the FRAP-induced activation of caspase-3 but not that of caspase-9, whereas the caspase-3 inhibitor, z-DEVD-fmk, markedly attenuated the FRAP-induced activation of caspase-8. In BALB/c(nu/nu) mice bearing a HeLa xenograft, FRAP dosed at 25 or 50mg/kg significantly inhibited tumor growth. CONCLUSION:Our results indicate caspase-mediated activation of the mitochondrial death pathway plays a critical role in the FRAP-induced apoptosis of HeLa cells and that FRAP inhibits the in vivo tumor growth of HeLa xenograft mice.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Ju HK,Lee HW,Chung KS,Choi JH,Cho JG,Baek NI,Chung HG,Lee KTdoi
10.1016/j.jep.2012.03.011subject
Has Abstractpub_date
2012-05-07 00:00:00pages
460-8issue
1eissn
0378-8741issn
1872-7573pii
S0378-8741(12)00169-9journal_volume
141pub_type
杂志文章abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Quercus Infectoria galls (QIG) have a long history of use in traditional Chinese medicine and traditional Uyghur medicine for the treatment of diarrhea, hemorrhage, skin disease, and many other human ailments. Medicinal applications of QIG have become increasingly popular in Greece, Asia ...
journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Meta-analysis (MA) on Chinese herbal medicine (CHM) trials is increasingly published and indexed in major international databases but their trustworthiness and clinical applicability is uncertain. We aimed to assess the characteristics and methodological quality of MA on CHM. MATERIALS A...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
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abstract::In this study, the effect of regular khat chewing on serum glucose and C-peptide levels were investigated in both healthy male individuals and type 2 diabetic patients. The results presented show the levels of glucose and C-peptide of healthy individuals to be non-significantly different between khat and non-khat chew...
journal_title:Journal of ethnopharmacology
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abstract::Impatiens pritzellii Hook. f. var. hupehensis Hook. f. (Balsaminaceae) has been well-known and widely used in China as an anti-rheumatoid arthritis (anti-RA) herb. In this present study, mice with collagen-induced arthritis (CIA) have been treated with the methanol (MeOH) extract (0.56, 1.12, 1.68 and 2.24 g/kg body w...
journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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abstract:AIM OF THE STUDY:The majority of people living in Kourittenga Province, Burkina Faso, are highly dependent on medicinal plants for their daily health care. Knowledge on the use of medicinal plants by traditional healers is being seriously threatened, due to the fact that it is commonly transferred from one generation t...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Type 2 diabetes mellitus (T2DM) regarded as a "hot" disease in traditional Chinese medicine (TCM). Accordingly, TCM uses a cold drug or formula such as the Chinese herbal formulae "Yitangkang" (YTK) as a treatment. YTK exhibited a good clinical antidiabetic effect in several experiments. ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2019-07-15 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2012-10-31 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2010-01-08 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2015-11-04 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2012-03-27 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2005-02-10 00:00:00
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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更新日期:1995-05-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2013-10-28 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2005-05-13 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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