Abstract：
:The purpose of this study was to investigate the feasibility and advantages of constructing a novel tissue engineering bone, using β-tricalcium phosphate (β-TCP) and rat bone marrow mesenchymal stem cells (MSCs), modified with human bone morphogenetic protein 2 gene (hBMP2) and human vascular endothelial growth factor 165 gene (hVEGF165), through lentiviral transfection. Both genes were successfully co-expressed in the co-transfection group for up to eight weeks confirmed by enzyme-linked immunosorbent assay (ELISA). After seeding MSCs onto the scaffolds, scanning electron microscopy (SEM) observation showed that MSCs grew and proliferated well in co-transfection group at 7 and 14 days. There was no significant difference among all the groups in hoechst DNA assay for cell proliferation for 14 days after cell seeding (P > 0.05), but the highest alkaline phosphatase (ALP) activity was observed in the co-transfection group at 14 days after cell seeding (p < 0.01). These results demonstrated that it was advantageous to construct tissue engineering bone using β-TCP combined with MSCs lentivirally co-transfected with BMP2 and VEGF165, providing an innovative way for treating bone defects.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Jiang J,Fan CY,Zeng BFdoi
10.3390/ijms12031744subject
Has Abstractpub_date
2011-01-01 00:00:00pages
1744-55issue
3issn
1422-0067pii
ijms-12-01744journal_volume
12pub_type
杂志文章abstract：:The deubiquitination of histone H2A on lysine 119 by 2A-DUB/MYSM1, BAP1, USP16, and other enzymes is required for key cellular processes, including transcriptional activation, apoptosis, and cell cycle control, during normal hematopoiesis and tissue development, and in tumor cells. Based on our finding that MYSM1 colo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21113762
更新日期：2020-05-26 00:00:00
abstract：:Inorganic phosphate (Pi) is an essential nutrient for the maintenance of cells. In healthy mammals, extracellular Pi is maintained within a narrow concentration range of 0.70 to 1.55 mM. Mammalian cells depend on Na+/Pi cotransporters for Pi absorption, which have been well studied. However, a new type of sodium-indep...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21239298
更新日期：2020-12-06 00:00:00
abstract：:Integrins are important regulators of cell survival, proliferation, adhesion and migration. Once activated, integrins establish a regulated link between the extracellular matrix and the cytoskeleton. Integrins have well-established functions in cancer, such as in controlling cell survival by engagement of many specifi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18010189
更新日期：2017-01-18 00:00:00
abstract：:Lysozyme is widely used as a model protein in studies of structure-function relationships. Recently, lysozyme has gained attention for use in accelerating the degradation of secondary sludge, which mainly consists of bacteria. However, a high-throughput screening system for lysozyme engineering has not been reported. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228668
更新日期：2020-11-17 00:00:00
abstract：:Extracellular vesicles (EVs), including exosomes and microvesicles, are nano-to-micrometer vesicles released from nearly all cellular types. EVs comprise a mixture of bioactive molecules (e.g., mRNAs, miRNAs, lipids, and proteins) that can be transported to the targeted cells/tissues via the blood or lymph circulation...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145163
更新日期：2020-07-21 00:00:00
abstract：:Citrate is a key intermediate of the tricarboxylic acid cycle and acts as an allosteric signal to regulate the production of cellular ATP. An elevated cytosolic citrate concentration inhibits growth in several types of human cancer cells; however, the underlying mechanism by which citrate induces the growth arrest of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092105
更新日期：2019-04-29 00:00:00
abstract：:Mercaptopyruvate sulfurtransferase (Mpst) and its homolog thiosulfate sulfurtransferase (Tst = rhodanese) detoxify cyanide to thiocyanate. Mpst is attracting attention as one of the four endogenous hydrogen sulfide (H2S)/reactive sulfur species (RSS)-producing enzymes, along with cystathionine β-synthase (Cbs), cystat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21030818
更新日期：2020-01-27 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is one of the leading presentations of head and neck cancer (HNC). The first part of this review will describe the highlights of the oral microbiome in health and normal development while demonstrating how both the oral and gut microbiome can map OSCC development, progression, treat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218061
更新日期：2020-10-29 00:00:00
abstract：:Cementum is a mineralized layer on the tooth's root surface and facilitates the biomechanical anchoring of fibrous connective tissues as a part of tooth-supportive complexes. Previously, we observed that OCCM30 cementoblasts cultured on fibrin matrices underwent apoptosis due to fibrin degradation through the expressi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112380
更新日期：2017-11-09 00:00:00
abstract：:The market success of any rice cultivar is exceedingly dependent on its grain appearance, as well as its grain yield, which define its demand by consumers as well as growers. The present study was undertaken to explore the contribution of nine major genes, qPE9~1, GW2, SLG7, GW5, GS3, GS7, GW8, GS5, and GS2, in regula...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194824
更新日期：2019-09-28 00:00:00
abstract：:Sclerotinia sclerotiorum (Ss) is a devastating fungal pathogen that causes Sclerotinia stem rot in rapeseed (Brassica napus), and is also detrimental to mulberry and many other crops. A wild mulberry germplasm, Morus laevigata, showed high resistance to Ss, but the molecular basis for the resistance is largely unknown...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218358
更新日期：2020-11-07 00:00:00
abstract：:Eosinophilic colitis (EC) is a common cause of haematochezia in infants and young children. The exact pathomechanism is not understood, and the diagnosis is challenging. The role of microRNAs as key class of regulators of mRNA expression and translation in patients with EC has not been explored. Therefore, the aim of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051050
更新日期：2017-05-12 00:00:00
abstract：:Consecutive treatment of adult rat heart with isoproterenol and adenosine (Iso/Aden), known to consecutively activate PKA/PKC signaling, is cardioprotective against ischemia and reperfusion (I/R). Whether this is cardioprotective in an immature heart is unknown. Langendorff-perfused hearts from adult and immature (60 ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020494
更新日期：2018-02-07 00:00:00
abstract：:Metronidazole (MD) is known as a periodontitis medicine and has been widely used in antibiotics for resistance to anaerobic bacteria, periodontal disease, and other threats. To treat diseases, drug delivery carriers are needed with a high bioadhesive property and enhanced drug penetration. Poly (acrylic acid) (PAA) hy...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010187
更新日期：2019-12-26 00:00:00
abstract：:High levels of docosahexaenoic acid (DHA) in the phospholipids of mammalian brain have generated increasing interest in the search for its role in regulating brain functions. Recent studies have provided evidence for enhanced protective effects when DHA is administered in combination with phytochemicals, such as querc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20040932
更新日期：2019-02-21 00:00:00
abstract：:The conserved serine/threonine kinase mTOR (the mammalian target of rapamycin), a downstream effector of the PI3K/AKT pathway, forms two distinct multiprotein complexes: mTORC1 and mTORC2. mTORC1 is sensitive to rapamycin, activates S6K1 and 4EBP1, which are involved in mRNA translation. It is activated by diverse sti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms13021886
更新日期：2012-01-01 00:00:00
abstract：:The life of any living organism can be defined as a hurdle due to different kind of stresses. As with all living organisms, plants are exposed to various abiotic stresses, such as drought, salinity, extreme temperatures and chemical toxicity. These primary stresses are often interconnected, and lead to the overproduct...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms13044458
更新日期：2012-01-01 00:00:00
abstract：:Asymmetric dimethylarginine (ADMA) is an endogenous nitric oxide synthase (NOS) inhibitor known as a mediator of endothelial dysfunction and atherosclerosis. Circulating ADMA levels are correlated with cardiovascular risk factors such as hypercholesterolemia, arterial hypertension, diabetes mellitus, hyperhomocysteine...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15046391
更新日期：2014-04-15 00:00:00
abstract：:The largest group of deubiquitinases-ubiquitin-specific proteases (UBPs)-perform extensive and significant roles in plants, including the regulation of development and stress responses. A comprehensive analysis of UBP genes has been performed in Arabidopsis thaliana, but no systematic study has been conducted in moso ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174309
更新日期：2019-09-03 00:00:00
abstract：:The drought is a crucial environmental factor that determines yielding of many crop species, e.g., Fabaceae, which are a source of valuable proteins for food and feed. Herein, we focused on the events accompanying drought-induced activation of flower abscission zone (AZ)-the structure responsible for flower detachment...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20153731
更新日期：2019-07-31 00:00:00
abstract：:In wheat, flag leaf, stem, chaff and awns contribute to grain filling through photosynthesis and/or re-mobilization. Environmental and genetic effects on the relative contribution of each organ were examined by analyzing the consequences of sink-source manipulations (shading and excision) and by comparing carbon isoto...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010056
更新日期：2017-12-25 00:00:00
abstract：:Rates of loco-regional recurrence and distant metastasis remain high among head and neck squamous cell carcinoma (HNSCC) patients, despite advancing cancer treatment modalities and therapeutic agents. One area that has generated considerable interest is the immune landscape of the tumour, heralding a wave of immune ch...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218229
更新日期：2020-11-03 00:00:00
abstract：:Maize (Zea mays L.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. A considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161226106
更新日期：2015-11-30 00:00:00
abstract：:The use of animal models in fundamental or pre-clinical research remains an absolute requirement for understanding human pathologies and developing new drugs. In order to transpose these results into clinical practice, many parameters must be taken into account to limit bias. Attention has recently been focused on the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020774
更新日期：2021-01-14 00:00:00
abstract：:Agricultural production continues to be constrained by a number of biotic and abiotic factors that can reduce crop yield quantity and quality. Potassium (K) is an essential nutrient that affects most of the biochemical and physiological processes that influence plant growth and metabolism. It also contributes to the s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14047370
更新日期：2013-04-02 00:00:00
abstract：:In this article we propose a systematic development method for rational drug design while reviewing paradigms in industry, emerging techniques and technologies in the field. Although the process of drug development today has been accelerated by emergence of computational methodologies, it is a herculean challenge requ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms12042262
更新日期：2011-01-01 00:00:00
abstract：:Carvacrol is a natural compound known to be a highly effective antibacterial; however, it is a hydrophobic molecule, which is a limitation to its use within food packaging. Flaxseed gum (FG) films containing different contents of carvacrol (C) were produced by a film-casting method with sonication. The effects of sonic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051637
更新日期：2020-02-28 00:00:00
abstract：:Bacterial exopolysaccharides produced by lactic acid bacteria are of increasing interest in the food industry, since they might enhance the technological and functional properties of some edible matrices. In this work, Pediococcus parvulus 2.6, which produces an O2-substituted (1,3)-β-d-glucan exopolysaccharide only s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18071588
更新日期：2017-07-21 00:00:00
abstract：:It has been well established that hypoxia significantly increases both cellular and tumor resistance to ionizing radiation. Hypoxia associated radiation resistance has been known for some time but there has been limited success in sensitizing cells to radiation under hypoxic conditions. These studies show that, when i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082228
更新日期：2018-07-30 00:00:00
abstract：:The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism-luminous bacteria-under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was consider...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186783
更新日期：2020-09-16 00:00:00
