Electroporation-mediated gene transfer of SOX trio to enhance chondrogenesis in adipose stem cells.


OBJECTIVE:The aim of the present study was to determine if the electroporation-mediated gene transfer of SOX trio enhances the chondrogenic potential of adipose stem cells (ASCs). DESIGN:ASCs were transfected with SOX trio genes using an electroporation technique and cultured for 3 weeks. The pellets were analyzed for DNA and glycosaminoglycan (GAG) analysis, and the gene and protein expression of SOX-5, SOX-6, SOX-9, type 1 collagen (COL1Al), type 2 collagen (COL2Al) and type 10 collagen (COL10A1) using real-time PCR and Western blot analysis. Further in vivo studies were carried out by subcutaneous transplantation of pellets in severe combined immunodeficiency (SCID) mice for 3 weeks. RESULTS:The gene transfer efficiency was high (approximately 70%). Transfected ASCs showed high expression of corresponding genes after 21 days, and each SOX protein was detected in ASCs transfected with the corresponding gene. The chondrogenic differentiation of ASCs, as demonstrated by GAG levels and Safranin-O staining, showed significant enhancement when SOX trio were co-transfected, while subsets with single gene transfer of SOX-5, -6, or -9 did not show significant elevation. SOX trio co-transfection enhanced COL2A1 mRNA, but did not increase COL1A1 and COL10A1 mRNA. Type II collagen protein dramatically increased, and type X collagen decreased with co-transfection of the SOX trio. When pellets were implanted in the subcutaneous pouch of SCID mice for 3 weeks, ASCs co-transfected with SOX trio demonstrated abundant proteoglycan, significantly reduced mineralization. CONCLUSION:The electroporation-mediated transfection of SOX trio greatly enhances chondrogenesis from ASCs, while decreasing hypertrophy.


Im GI,Kim HJ




Has Abstract


2011-04-01 00:00:00














  • Systematic review and meta-analysis of the reliability and discriminative validity of cartilage compositional MRI in knee osteoarthritis.

    abstract:OBJECTIVE:To assess reliability and discriminative validity of cartilage compositional magnetic resonance imaging (MRI) in knee osteoarthritis (OA). DESIGN:The study was carried out per PRISMA recommendations. We searched MEDLINE and EMBASE (1974 - present) for eligible studies. We performed qualitative synthesis of r...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析


    authors: MacKay JW,Low SBL,Smith TO,Toms AP,McCaskie AW,Gilbert FJ

    更新日期:2018-09-01 00:00:00

  • Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells?

    abstract:OBJECTIVE:Adipose tissue-derived mesenchymal stem cells (ATMSCs) have been shown to differentiate into bone, cartilage, fat or muscle. However, it is not certain that ATMSCs are equal to bone marrow-derived mesenchymal stem cells (BMMSC) for their bone and cartilage forming potential. The purpose of this study was to a...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Im GI,Shin YW,Lee KB

    更新日期:2005-10-01 00:00:00

  • Associations between pre-operative radiographic changes and outcomes after total knee joint replacement for osteoarthritis.

    abstract:OBJECTIVE:To assess the influence of pre-operative X-ray changes on the response to total knee joint replacement (TKR). METHODS:We included patients from one centre who underwent primary TKR (n = 478) for osteoarthritis in 2006 and 2007. The International Knee Society score (IKSS) and short form health survey were col...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Dowsey MM,Nikpour M,Dieppe P,Choong PF

    更新日期:2012-10-01 00:00:00

  • Osteoarthritis year 2010 in review: pathomechanisms.

    abstract::Osteoarthritis is characterized by progressive breakdown of articular cartilage. This review summarizes findings of the last year, which shed new light on mechanisms and factors involved in cartilage loss. Evidence is accumulating that the transcription factor hypoxia-inducible factor-2α (HIF-2α) is highly enhanced in...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: van den Berg WB

    更新日期:2011-04-01 00:00:00

  • Measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis: a systematic review.

    abstract:OBJECTIVES:To systematically appraise the evidence on measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis (OA). METHODS:Electronic searches were performed in MEDLINE, CINAHL, Scopus and SPORT...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Kroman SL,Roos EM,Bennell KL,Hinman RS,Dobson F

    更新日期:2014-01-01 00:00:00

  • The longitudinal relationship between hand, hip and knee osteoarthritis and cardiovascular events: a population-based cohort study.

    abstract:OBJECTIVE:In this population-based cohort study, we examined the association between the presence of symptomatic osteoarthritis (OA) and risk for cardiovascular (CV) events. METHOD:A cohort aged ≥55 years recruited from 1996 to 98 was followed through provincial health administrative data to 2014. Demographics, joint ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kendzerska T,Jüni P,King LK,Croxford R,Stanaitis I,Hawker GA

    更新日期:2017-11-01 00:00:00

  • Analysis of ADAMTS4 and MT4-MMP indicates that both are involved in aggrecanolysis in interleukin-1-treated bovine cartilage.

    abstract:OBJECTIVE:To investigate the mechanism of aggrecanolysis in interleukin-1 (IL-1)-treated cartilage tissue by examining the time course of aggrecan cleavages and the tissue and medium content of membrane type 4-matrix metalloproteinases (MT4-MMP) and a disintegrin and metalloproteinase with thrombospondin type I motifs ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Patwari P,Gao G,Lee JH,Grodzinsky AJ,Sandy JD

    更新日期:2005-04-01 00:00:00

  • The evolving large-strain shear responses of progressively osteoarthritic human cartilage.

    abstract:OBJECTIVE:The composition and structure of articular cartilage evolves during the development and progression of osteoarthritis (OA) resulting in changing mechanical responses. We aimed to assess the evolution of the intrinsic, large-strain mechanics of human articular cartilage-governed by collagen and proteoglycan an...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Maier F,Lewis CG,Pierce DM

    更新日期:2019-05-01 00:00:00

  • Bone mineral distribution of the proximal tibia in gonarthrosis assessed in vivo by photon absorption.

    abstract::Regional bone mineral density (BMD, g/cm2) of the proximal tibia including the subchondral plate was measured in vivo by dual photon absorptiometry (DPA). Twenty-one women and six men with gonarthrosis (GA) were examined. Twenty-four healthy women served as controls for the women. The precision error of the BMD measur...

    journal_title:Osteoarthritis and cartilage

    pub_type: 临床试验,杂志文章


    authors: Madsen OR,Schaadt O,Bliddal H,Egsmose C,Sylvest J

    更新日期:1994-06-01 00:00:00

  • A quantitative metric for knee osteoarthritis: reference values of joint space loss.

    abstract:OBJECTIVES:Knee osteoarthritis (OA) onset and progression has been defined with transitions in Kellgren-Lawrence (KL) grade or Osteoarthritis Research Society International (OARSI) Joint Space Narrowing (JSN) grade. We quantitatively describe one-year transitions in KL grade and JSN, using fixed joint space width (fJSW...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ratzlaff C,Ashbeck EL,Guermazi A,Roemer FW,Duryea J,Kwoh CK

    更新日期:2018-09-01 00:00:00

  • Human genome-wide expression analysis reorients the study of inflammatory mediators and biomechanics in osteoarthritis.

    abstract::A major objective of this article is to examine the research implications of recently available genome-wide expression profiles of cartilage from human osteoarthritis (OA) joints. We propose that, when viewed in the light of extensive earlier work, this novel data provides a unique opportunity to reorient the design o...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Sandy JD,Chan DD,Trevino RL,Wimmer MA,Plaas A

    更新日期:2015-11-01 00:00:00

  • A static, closed and scaffold-free bioreactor system that permits chondrogenesis in vitro.

    abstract:OBJECTIVE:To characterise in vitro engineered cartilaginous constructs made employing a novel static, scaffold-free and closed chamber system. DESIGN:Chondrocytes derived from slaughter age pigs (3-6 months) were seeded at high density (20 x 10(6)) into cylindrical chambers (1.0 x 0.5cm) and were maintained between an...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Grogan SP,Rieser F,Winkelmann V,Berardi S,Mainil-Varlet P

    更新日期:2003-06-01 00:00:00

  • Preoperative progressive explosive-type resistance training is feasible and effective in patients with hip osteoarthritis scheduled for total hip arthroplasty--a randomized controlled trial.

    abstract:OBJECTIVE:To investigate the efficacy and feasibility of progressive explosive-type resistance training (RT) in patients with osteoarthritis (OA) of the hip scheduled for total hip arthroplasty (THA). METHOD:Randomized controlled trial (1:1) in patients diagnosed with hip OA and scheduled for THA. The intervention gro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Hermann A,Holsgaard-Larsen A,Zerahn B,Mejdahl S,Overgaard S

    更新日期:2016-01-01 00:00:00

  • Should subchondral bone turnover be targeted when treating osteoarthritis?

    abstract:OBJECTIVE:Osteoarthritis (OA) is the most common form of arthritic disease, and it is a major cause of disability and impaired quality of life in the elderly. OA is a complex disease of the entire joint, including bone and cartilage, thereby presenting alternative approaches for treatment. This review summarizes emergi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Karsdal MA,Leeming DJ,Dam EB,Henriksen K,Alexandersen P,Pastoureau P,Altman RD,Christiansen C

    更新日期:2008-06-01 00:00:00

  • Subchondral bone marrow lesions are highly associated with, and predict subchondral bone attrition longitudinally: the MOST study.

    abstract:OBJECTIVE:Subchondral bone attrition (SBA) is defined as flattening or depression of the osseous articular surface. The causes of attrition are unknown, but remodeling processes due to chronic overload that are reflected as bone marrow edema-like lesions (BMLs) on magnetic resonance imaging (MRI) might predispose the s...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究


    authors: Roemer FW,Neogi T,Nevitt MC,Felson DT,Zhu Y,Zhang Y,Lynch JA,Javaid MK,Crema MD,Torner J,Lewis CE,Guermazi A

    更新日期:2010-01-01 00:00:00

  • Impaired muscle function in a mouse surgical model of post-traumatic osteoarthritis.

    abstract:OBJECTIVES:Using a mouse surgical model of post-traumatic osteoarthritis (OA), we sought to determine if muscle function is altered following acute joint injury and whether this relates to OA progression. DESIGN:Male C57BL/6 mice underwent surgical transection of the medio-meniscal tibial ligament destabilisation of t...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: van der Poel C,Levinger P,Tonkin BA,Levinger I,Walsh NC

    更新日期:2016-06-01 00:00:00

  • Vital marking of articular chondrocytes by retroviral infection using green fluorescence protein.

    abstract:OBJECTIVE:One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluor...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Hirschmann F,Verhoeyen E,Wirth D,Bauwens S,Hauser H,Rudert M

    更新日期:2002-02-01 00:00:00

  • Relationship between weight loss in obese knee osteoarthritis patients and serum biomarkers of cartilage breakdown: secondary analyses of a randomised trial.

    abstract:OBJECTIVE:To explore effects of weight loss and maintenance on serum cartilage biomarkers denaturation neoepitope for Collagen2 (Coll2-1) and Fibulin3 fragment (Fib3-2), as well as correlations between Coll2-1 and Fib3-2 and symptomatic improvement, in a knee osteoarthritis (KOA) population. DESIGN:192 obese KOA patie...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Bartels EM,Henrotin Y,Bliddal H,Centonze P,Henriksen M

    更新日期:2017-10-01 00:00:00

  • Epigenetically mediated spontaneous reduction of NFAT1 expression causes imbalanced metabolic activities of articular chondrocytes in aged mice.

    abstract:OBJECTIVE:Abnormal metabolic activities of chondrocytes may cause articular cartilage (AC) degradation, but key transcription factors regulating metabolic activities in AC of aging individuals remain unknown. This study aimed to investigate the role of transcription factor NFAT1 in regulating the expression of anabolic...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Zhang M,Lu Q,Egan B,Zhong XB,Brandt K,Wang J

    更新日期:2016-07-01 00:00:00

  • Poor replicability of recommended exercise interventions for knee osteoarthritis: a descriptive analysis of evidence informing current guidelines and recommendations.

    abstract:OBJECTIVE:To examine the reporting completeness of exercise-based interventions for knee osteoarthritis (OA) in studies that form the basis of current clinical guidelines, and examine if the clinical benefit (pain and disability) from exercise is associated with the intervention reporting completeness. DESIGN:Review o...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析,评审


    authors: Bartholdy C,Nielsen SM,Warming S,Hunter DJ,Christensen R,Henriksen M

    更新日期:2019-01-01 00:00:00

  • Osteoarthritis biomarkers: year in review.

    abstract:OBJECTIVE:To summarise important findings from biomarker studies relevant to osteoarthritis (OA), published between April 2016 and March 2017; to consider these findings in the context of new discoveries and technologies, and clinical and scientific need in OA. DESIGN:Studies were selected by PubMed search, conducted ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Watt FE

    更新日期:2018-03-01 00:00:00

  • Synovial incorporation of polyacrylamide hydrogel after injection into normal and osteoarthritic animal joints.

    abstract:OBJECTIVE:Polyacrylamide hydrogel (PAAG) is a non-toxic, non-degradable synthetic product, used for years in the augmentation of soft tissues. Preliminary results in animals and humans have suggested long-lasting beneficial effects on symptoms of osteoarthritis (OA). The aim of this histopathological study was to inves...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Christensen L,Camitz L,Illigen KE,Hansen M,Sarvaa R,Conaghan PG

    更新日期:2016-11-01 00:00:00

  • Macroscopic cartilage repair scoring of defect fill, integration and total points correlate with corresponding items in histological scoring systems - a study in adult sheep.

    abstract:OBJECTIVE:To correlate osteochondral repair assessed by validated macroscopic scoring systems with established semiquantitative histological analyses in an ovine model and to test the hypothesis that important macroscopic individual categories correlate with their corresponding histological counterparts. METHODS:In th...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Goebel L,Orth P,Cucchiarini M,Pape D,Madry H

    更新日期:2017-04-01 00:00:00

  • The impact of a significant weight loss on inflammation assessed on DCE-MRI and static MRI in knee osteoarthritis: a prospective cohort study.

    abstract:OBJECTIVE:To study the impact of weight loss on inflammation in individuals with overweight and knee osteoarthritis (OA) using both static- and dynamic contrast-enhanced (DCE)-MRI and assess the association of these changes to pain. DESIGN:Individuals with overweight (BMI > 27) and knee OA were examined before and aft...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Daugaard CL,Henriksen M,Riis RGC,Bandak E,Nybing JD,Hangaard S,Bliddal H,Boesen M

    更新日期:2020-06-01 00:00:00

  • Magnetic resonance imaging (MRI) of articular cartilage in knee osteoarthritis (OA): morphological assessment.

    abstract:OBJECTIVE:Magnetic resonance imaging (MRI) is a three-dimensional imaging technique with unparalleled ability to evaluate articular cartilage. This report reviews the current status of morphological assessment of cartilage with quantitative MRI (qMRI), and its relevance for identifying disease status, and monitoring pr...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Eckstein F,Cicuttini F,Raynauld JP,Waterton JC,Peterfy C

    更新日期:2006-01-01 00:00:00

  • Differential induction and regulation of matrix metalloproteinases in osteoarthritic tissue and fluid synovial fibroblasts.

    abstract:OBJECTIVES:To investigate the secretion profiles of matrix metalloproteinases (MMP) and their inhibitors (TIMP) in synovial fluid-derived fibroblasts and to compare them with those of tissue-derived fibroblasts. METHODS:Fibroblast cultures established from synovial tissues (TSC) and fluids (FSC) of the same OA patient...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fuchs S,Skwara A,Bloch M,Dankbar B

    更新日期:2004-05-01 00:00:00

  • Expression of biglycan, decorin and fibromodulin in the hypertrophic phase of experimental osteoarthritis.

    abstract::This study sought to assess the relative levels of the mRNAs of the core proteins of the small proteoglycans (PGs) biglycan, decorin and fibromodulin in the hypertrophic phase of the early osteoarthritis (OA) that follows joint injury. Experimental OA was induced in eight dogs by transection of the anterior cruciate l...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Dourado GS,Adams ME,Matyas JR,Huang D

    更新日期:1996-09-01 00:00:00

  • Relationship between anisotropic diffusion properties and tissue morphology in porcine TMJ disc.

    abstract:OBJECTIVE:To investigate the relationship between anisotropic solute diffusion properties and tissue morphology in porcine temporomandibular joint (TMJ) discs. DESIGN:TMJ discs from eleven pigs aged 6-8 months were divided into five regions: anterior, intermediate, posterior, lateral, and medial. The transport propert...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Shi C,Wright GJ,Ex-Lubeskie CL,Bradshaw AD,Yao H

    更新日期:2013-04-01 00:00:00

  • Trabecular bone structure and spatial differences in articular cartilage MR relaxation times in individuals with posterior horn medial meniscal tears.

    abstract:OBJECTIVE:To analyze knee trabecular bone structure and spatial cartilage T(1ρ) and T(2) relaxation times using 3-T magnetic resonance imaging (MRI) in subjects with and without tears of posterior horn of the medial meniscus (PHMM). DESIGN:3-T MRI from 59 subjects (>18 years), were used to evaluate PHMM tears based on...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kumar D,Schooler J,Zuo J,McCulloch CE,Nardo L,Link TM,Li X,Majumdar S

    更新日期:2013-01-01 00:00:00

  • Expression of microRNAs during chondrogenesis of human adipose-derived stem cells.

    abstract:OBJECTIVES:MicroRNAs (miRNAs) play an important role in the regulation of chondrogenesis of mesenchymal stem cells, but their expression still remains unknown in human adipose-derived stem cells (hADSCs). In this study the miRNA expression profile during chondrogenic differentiation of hADSC and the potential mechanism...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Zhang Z,Kang Y,Zhang Z,Zhang H,Duan X,Liu J,Li X,Liao W

    更新日期:2012-12-01 00:00:00