Abstract:
:The charge-stabilization effect associated with alpha-helices in proteins has been reexamined by microscopic calculations without any a priori assumptions about the dielectric constant of the protein. The calculations reproduce the observed charge stabilization effect of a helix in two well-defined test cases: a histidine residue situated at the C-terminal end of a helix in barnase and the sulfate ligand located near the N-terminal end of a helix of the sulfate-binding protein. They also show that the effective dielectric constant for helix-charge interactions is much larger than previously assumed and that the stabilizing effect of the helix is not associated with the helix macrodipole but rather with a few localized dipoles confined mostly to the first turn of the helix. It is predicted that mutations at one end of the helix should have very small effects on the stabilization of charges at the opposite terminus. It is pointed out that the relatively short-ranged effect of the helix is essentially similar to other cases in which localized dipoles play key roles in electrostatic stabilization.
journal_name
Proc Natl Acad Sci U S Aauthors
Aqvist J,Luecke H,Quiocho FA,Warshel Adoi
10.1073/pnas.88.5.2026subject
Has Abstractpub_date
1991-03-01 00:00:00pages
2026-30issue
5eissn
0027-8424issn
1091-6490journal_volume
88pub_type
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