Abstract:
BACKGROUND:We have reported that doxycycline-induced over-expression of wild type prion protein (PrP) in skeletal muscles of Tg(HQK) mice is sufficient to cause a primary myopathy with no signs of peripheral neuropathy. The preferential accumulation of the truncated PrP C1 fragment was closely correlated with these myopathic changes. In this study we use gene expression profiling to explore the temporal program of molecular changes underlying the PrP-mediated myopathy. RESULTS:We used DNA microarrays, and confirmatory real-time PCR and Western blot analysis to demonstrate deregulation of a large number of genes in the course of the progressive myopathy in the skeletal muscles of doxycycline-treated Tg(HQK) mice. These include the down-regulation of genes coding for the myofibrillar proteins and transcription factor MEF2c, and up-regulation of genes for lysosomal proteins that is concomitant with increased lysosomal activity in the skeletal muscles. Significantly, there was prominent up-regulation of p53 and p53-regulated genes involved in cell cycle arrest and promotion of apoptosis that paralleled the initiation and progression of the muscle pathology. CONCLUSION:The data provides the first in vivo evidence that directly links p53 to a wild type PrP-mediated disease. It is evident that several mechanistic features contribute to the myopathy observed in PrP over-expressing mice and that p53-related apoptotic pathways appear to play a major role.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Liang J,Parchaliuk D,Medina S,Sorensen G,Landry L,Huang S,Wang M,Kong Q,Booth SAdoi
10.1186/1471-2164-10-201subject
Has Abstractpub_date
2009-04-28 00:00:00pages
201issn
1471-2164pii
1471-2164-10-201journal_volume
10pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:Glutamine is a major amino donor for the synthesis of amino acids, nucleotides, and other nitrogen-containing compounds in all organisms. In addition to its role in nutrition and metabolism, glutamine can also function as a signaling molecule in bacteria, yeast, and humans. By contrast, the functions of glut...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1892-7
更新日期:2015-09-25 00:00:00
abstract:BACKGROUND:Tea is one of the most popular non-alcoholic beverages worldwide. However, the tea plant, Camellia sinensis, is difficult to culture in vitro, to transform, and has a large genome, rendering little genomic information available. Recent advances in large-scale RNA sequencing (RNA-seq) provide a fast, cost-eff...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-131
更新日期:2011-02-28 00:00:00
abstract:BACKGROUND:Skeletal muscle mass can be markedly reduced through a process called atrophy, as a consequence of many diseases or critical physiological and environmental situations. Atrophy is characterised by loss of contractile proteins and reduction of fiber volume. Although in the last decade the molecular aspects un...
journal_title:BMC genomics
pub_type: 杂志文章,meta分析
doi:10.1186/1471-2164-9-630
更新日期:2008-12-23 00:00:00
abstract:BACKGROUND:The ability to rapidly map millions of oligonucleotide fragments to a reference genome is crucial to many high throughput genomic technologies. RESULTS:We propose an intuitive and efficient algorithm, titled extreme MApping of OligoNucleotide (xMAN), to rapidly map millions of oligonucleotide fragments to a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-S1-S20
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:The recent advances in next generation sequencing technology have made the sequencing of RNA (i.e., RNA-Seq) an extemely popular approach for gene expression analysis. Identification of significant differential expression represents a crucial initial step in these analyses, on which most subsequent inference...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2848-2
更新日期:2016-08-04 00:00:00
abstract:BACKGROUND:The sustainability of poultry farming relies on the development of more efficient and autonomous production systems in terms of feed supply. This implies a better integration of adaptive traits in breeding programs, including digestive efficiency, in order to favor the use of a wider variety of feedstuffs. T...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5344-z
更新日期:2018-12-13 00:00:00
abstract:BACKGROUND:The purpose of this research was to develop a novel information theoretic method and an efficient algorithm for analyzing the gene-gene (GGI) and gene-environmental interactions (GEI) associated with quantitative traits (QT). The method is built on two information-theoretic metrics, the k-way interaction inf...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-509
更新日期:2009-11-04 00:00:00
abstract:BACKGROUND:Phenylalanine ammonia lyase (PAL) is a key enzyme of the phenylpropanoid pathway that catalyzes the deamination of phenylalanine to trans-cinnamic acid, a precursor for the lignin and flavonoid biosynthetic pathways. To date, PAL genes have been less extensively studied in gymnosperms than in angiosperms. Ou...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S3-S1
更新日期:2012-06-11 00:00:00
abstract:BACKGROUND:The ultimate phenome of any organism is modulated by regulated transcription of many genes. Characterization of genetic makeup is thus crucial for understanding the molecular basis of phenotypic diversity, evolution and response to intra- and extra-cellular stimuli. Chickpea is the world's third most importa...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-415
更新日期:2009-09-05 00:00:00
abstract:BACKGROUND:Banana wilt disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), is one of the most devastating diseases in banana (Musa spp.). Foc is a soil borne pathogen that causes rot of the roots or wilt of leaves by colonizing the xylem vessels. The dual RNA sequencing is used to simultaneo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5902-z
更新日期:2019-06-24 00:00:00
abstract:BACKGROUND:Environmental heterogeneity continuously produces a selective pressure that results in genomic variation among organisms; understanding this relationship remains a challenge in evolutionary biology. Here, we evaluated the degree of genome-environmental association of seven stonefly species across a wide geog...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5453-3
更新日期:2019-01-24 00:00:00
abstract:BACKGROUND:The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-27
更新日期:2002-09-27 00:00:00
abstract:BACKGROUND:Development of the soil amoeba Dictyostelium discoideum is triggered by starvation. When placed on a solid substrate, the starving solitary amoebae cease growth, communicate via extracellular cAMP, aggregate by tens of thousands and develop into multicellular organisms. Early phases of the developmental prog...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1491-7
更新日期:2015-04-13 00:00:00
abstract:BACKGROUND:Development of crop varieties with high nitrogen use efficiency (NUE) is crucial for minimizing N loss, reducing environmental pollution and decreasing input cost. Maize is one of the most important crops cultivated worldwide and its productivity is closely linked to the amount of fertilizer used. A survey o...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-77
更新日期:2014-01-28 00:00:00
abstract:BACKGROUND:Genomic and transcriptomic sequence data are essential tools for tackling ecological problems. Using an approach that combines next-generation sequencing, de novo transcriptome assembly, gene annotation and synthetic gene construction, we identify and cluster the protein families from Favia corals from the n...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-546
更新日期:2013-08-12 00:00:00
abstract:BACKGROUND:Interactions between fish and pathogens, that may be harmless under natural conditions, often result in serious diseases in aquaculture systems. This is especially important due to the fact that the strains used in aquaculture are derived from wild strains that may not have had enough time to adapt to new di...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-541
更新日期:2011-11-02 00:00:00
abstract:BACKGROUND:The ascomycete fungus Ceratocystis cacaofunesta is the causal agent of wilt disease in cacao, which results in significant economic losses in the affected producing areas. Despite the economic importance of the Ceratocystis complex of species, no genomic data are available for any of its members. Given that ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-91
更新日期:2013-02-11 00:00:00
abstract:BACKGROUND:Plant microRNAs (miRNAs) are involved in post-transcriptional regulatory mechanisms of several processes, including the response to biotic and abiotic stress, often contributing to the adaptive response of the plant to adverse conditions. In addition to conserved miRNAs, found in a wide range of plant specie...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-41
更新日期:2012-01-24 00:00:00
abstract:BACKGROUND:Fusion genes are known to be drivers of many common cancers, so they are potential markers for diagnosis, prognosis or therapy response. The advent of paired-end RNA sequencing enhances our ability to discover fusion genes. While there are available methods, routine analyses of large number of samples are st...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5156-1
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Gene expression technologies have the ability to generate vast amounts of data, yet there often resides only limited resources for subsequent validation studies. This necessitates the ability to perform sorting and prioritization of the output data. Previously described methodologies have used functional pat...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-58
更新日期:2004-08-20 00:00:00
abstract:BACKGROUND:The advance of high-throughput sequencing has made it possible to obtain new transcriptomes and study splicing mechanisms in non-model organisms. In these studies, there is often a need to investigate the transcriptomes of two related organisms at the same time in order to find the similarities and differenc...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-16-S11-S5
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Horseradish peroxidases (HRPs) from Armoracia rusticana have long been utilized as reporters in various diagnostic assays and histochemical stainings. Regardless of their increasing importance in the field of life sciences and suggested uses in medical applications, chemical synthesis and other industrial ap...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-227
更新日期:2014-03-24 00:00:00
abstract:BACKGROUND:The process of alternative splicing provides a unique mechanism by which eukaryotes are able to produce numerous protein products from the same gene. Heightened variability in the proteome has been thought to potentiate increased behavioral complexity and response flexibility to environmental stimuli, thus c...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6600-6
更新日期:2020-03-23 00:00:00
abstract:BACKGROUND:The reconstruction of gene regulatory networks from high-throughput "omics" data has become a major goal in the modelling of living systems. Numerous approaches have been proposed, most of which attempt only "one-shot" reconstruction of the whole network with no intervention from the user, or offer only simp...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-S3-S17
更新日期:2009-12-03 00:00:00
abstract:BACKGROUND:We use an approach based on Factor Analysis to analyze datasets generated for transcriptional profiling. The method groups samples into biologically relevant categories, and enables the identification of genes and pathways most significantly associated to each phenotypic group, while allowing for the partici...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-6-109
更新日期:2005-08-17 00:00:00
abstract:BACKGROUND:Protein phosphorylation is responsible for a large portion of the regulatory functions of eukaryotic cells. Although the list of sequenced genomes of filamentous fungi has grown rapidly, the kinomes of recently sequenced species have not yet been studied in detail. The objective of this study is to apply a c...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-133
更新日期:2010-02-24 00:00:00
abstract:BACKGROUND:With the advance of new massively parallel genotyping technologies, quantitative trait loci (QTL) fine mapping and map-based cloning become more achievable in identifying genes for important and complex traits. Development of high-density genetic markers in the QTL regions of specific mapping populations is ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-469
更新日期:2010-08-11 00:00:00
abstract:BACKGROUND:Fusarium head blight (FHB), caused mainly by Fusarium graminearum (Fg) Schwabe (teleomorph: Gibberellazeae Schwble), brings serious damage to wheat production. Chinese wheat landrace Wangshuibai is one of the most important resistance sources in the world. The knowledge of mechanism underlying its resistance...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-197
更新日期:2013-03-21 00:00:00
abstract:BACKGROUND:Obesity, the excessive accumulation of body fat, is a highly heritable and genetically heterogeneous disorder. The complex, polygenic basis for the disease consisting of a network of different gene variants is still not completely known. RESULTS:In the current study we generated a BAC library of the obese-p...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3552-6
更新日期:2017-02-15 00:00:00
abstract:BACKGROUND:Democratising the growing body of whole genome sequencing data available for Triticum aestivum (bread wheat) has been impeded by the lack of a genome reference and the large computational requirements for analysing these data sets. RESULTS:DAWN (Diversity Among Wheat geNomes) integrates data from the T. aes...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5228-2
更新日期:2018-12-17 00:00:00