Abstract：
:Drebrin is a well-known side-binding protein of F-actin in the brain. Immunohistochemical data suggest that the peripheral parts of growing axons are enriched in the drebrin E isoform and mature axons are not. It has also been observed that drebrin E is concentrated in the growth cones of PC12 cells. These data strongly suggest that drebrin E plays a role in axonal growth during development. In this study, we used primary hippocampal neuronal cultures to analyze the role of drebrin E. Immunocytochemistry showed that within axonal growth cones drebrin E specifically localized to the transitional zone, an area in which dense networks of F-actins and microtubules overlapped. Over-expression of drebrin E caused drebrin E and F-actin to accumulate throughout the growth cone and facilitated axonal growth. In contrast, knockdown of drebrin E reduced drebrin E and F-actin in the growth cone and prevented axonal growth. Furthermore, inhibition of myosin II ATPase masked the promoting effects of drebrin E over-expression on axonal growth. These results suggest that drebrin E plays a role in axonal growth through actin-myosin interactions in the transitional zone of axonal growth cones.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Mizui T,Kojima N,Yamazaki H,Katayama M,Hanamura K,Shirao Tdoi
10.1111/j.1471-4159.2009.05993.xsubject
Has Abstractpub_date
2009-04-01 00:00:00pages
611-22issue
2eissn
0022-3042issn
1471-4159pii
JNC5993journal_volume
109pub_type
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1982.tb08697.x
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