Abstract:
:Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and single-virus tracking to investigate the cell entry, endocytic trafficking, and fusion behavior of DENV. Simultaneous tracking of DENV particles and various endocytic markers revealed that DENV enters cells exclusively via clathrin-mediated endocytosis. The virus particles move along the cell surface in a diffusive manner before being captured by a pre-existing clathrin-coated pit. Upon clathrin-mediated entry, DENV particles are transported to Rab5-positive endosomes, which subsequently mature into late endosomes through acquisition of Rab7 and loss of Rab5. Fusion of the viral membrane with the endosomal membrane was primarily detected in late endosomal compartments.
journal_name
PLoS Pathogjournal_title
PLoS pathogensauthors
van der Schaar HM,Rust MJ,Chen C,van der Ende-Metselaar H,Wilschut J,Zhuang X,Smit JMdoi
10.1371/journal.ppat.1000244subject
Has Abstractpub_date
2008-12-01 00:00:00pages
e1000244issue
12eissn
1553-7366issn
1553-7374journal_volume
4pub_type
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