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Effects of contaminated sediment from Cork Harbour, Ireland on the cytochrome P450 system of turbot.

Abstract：

:Hatchery-reared juvenile turbot (Scophthalmus maximus L.) were exposed for 3 weeks, under laboratory conditions, to inter-tidal sediments collected from polluted sites in Cork Harbour (Whitegate and Agahda) and a reference site at Ballymacoda Co., Cork, Ireland. The potential of the sediment exposure to induce cytochrome P450 activities and CYP1A1 in the fish was assessed. Chemical analysis revealed that the sediments originating from the reference and harbour sites were contaminated principally with PAHs-the harbour sites having double the levels of those at the reference site. Following 3 weeks exposure to the sediments western blotting demonstrated a strong immunogenic response for CYP1A1 in the liver, but not for gill or intestine. P450 activities were generally significantly higher than those exposed to reference site sediment. Liver was the most responsive tissue with significantly greater P450 activities compared with gill and intestinal tissues.

journal_name

Ecotoxicol Environ Saf

journal_title

Ecotoxicology and environmental safety

authors

Kilemade M,Hartl MG,O'Halloran J,O'Brien NM,Sheehan D,Mothersill C,van Pelt FN

doi

10.1016/j.ecoenv.2008.09.026

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

747-55

issue

3

eissn

0147-6513

issn

1090-2414

pii

S0147-6513(08)00234-0

journal_volume

72

pub_type

杂志文章

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