Thermodesulfovibrio aggregans sp. nov. and Thermodesulfovibrio thiophilus sp. nov., anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio.

Abstract:

:Four obligately anaerobic, thermophilic, sulfate-reducing bacterial strains, designated TGE-P1(T), TDV(T), TGL-LS1 and TSL-P1, were isolated from thermophilic (operated at 55 degrees C) methanogenic sludges from waste and wastewater treatment. The optimum temperature for growth of all the strains was in the range 55-60 degrees C. The four strains grew by reduction of sulfate with a limited range of electron donors, such as hydrogen, formate, pyruvate and lactate. In co-culture with the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus DeltaH(T), strains TGE-P1(T), TGL-LS1 and TSL-P1 were able to utilize lactate syntrophically for growth. The DNA G+C contents of all the strains were in the range 34-35 mol%. The major cellular fatty acids of the strains were iso-C(17 : 0), iso-C(16 : 0), C(16 : 0) and anteiso-C(15 : 0). Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strains belong to the Thermodesulfovibrio clade of the phylum 'Nitrospirae'. On the basis of their physiological, chemotaxonomic and genetic properties, strains TGL-LS1 (=JCM 13214) and TSL-P1 (=JCM 13215) were classified as strains of Thermodesulfovibrio islandicus. Two novel species of the genus Thermodesulfovibrio are proposed to accommodate the other two isolates: Thermodesulfovibrio aggregans sp. nov. (type strain TGE-P1(T) =JCM 13213(T) =DSM 17283(T)) and Thermodesulfovibrio thiophilus sp. nov. (type strain TDV(T) =JCM 13216(T) =DSM 17215(T)). To examine the ecological aspects of Thermodesulfovibrio-type cells in the sludge from which the strains were originally isolated, an oligonucleotide probe targeting 16S rRNA of all Thermodesulfovibrio species was designed and applied to thin sections of thermophilic sludge granules. Fluorescence in situ hybridization using the probe revealed rod- or vibrio-shaped cells as a significant population within the sludge, indicating their important role in the original ecosystem.

authors

Sekiguchi Y,Muramatsu M,Imachi H,Narihiro T,Ohashi A,Harada H,Hanada S,Kamagata Y

doi

10.1099/ijs.0.2008/000893-0

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

2541-8

issue

Pt 11

eissn

1466-5026

issn

1466-5034

pii

58/11/2541

journal_volume

58

pub_type

杂志文章
  • Phylogenetic relationships of necrogenic Erwinia and Brenneria species as revealed by glyceraldehyde-3-phosphate dehydrogenase gene sequences.

    abstract::Recent examination of the relationships of the dry necrosis-inducing (necrogenic) erwinias using 16S rDNA sequences demonstrated that these bacteria comprise a polyphyletic group and, therefore, have been subdivided into three distinct genera, Erwinia, Brenneria and Pectobacterium, with the classical 'amylovora' group...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-6-2057

    authors: Brown EW,Davis RM,Gouk C,van der Zwet T

    更新日期:2000-11-01 00:00:00

  • Solitalea longa sp. nov., isolated from freshwater and emended description of the genus Solitalea.

    abstract::A Gram-negative, strictly aerobic, oxidase-positive, catalase-negative and yellow-pigmented bacterium, designated strain HR-AVT, was isolated from a water sample of the Han River. Cells were elongated rods with gliding motility without flagellum. Growth was observed at 5-30 °C (optimum, 20 °C), pH 7-8 and 0-0.5 % NaCl...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002903

    authors: Lee Y,Jeon CO

    更新日期:2018-09-01 00:00:00

  • Bacillus oleivorans sp. nov., a diesel oil-degrading and solvent-tolerant bacterium.

    abstract::Two Gram-stain-positive, diesel oil-degrading, solvent-tolerant, aerobic, endospore-forming, rod-shaped bacteria were isolated from a contaminated laboratory plate. Based on 16S rRNA gene sequence analysis, strains JC228(T) and JC279 were identified as belonging to the genus Bacillus within the family Bacillaceae of t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000103

    authors: Azmatunnisa M,Rahul K,Subhash Y,Sasikala C,Ramana CV

    更新日期:2015-04-01 00:00:00

  • Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum.

    abstract::An obligately anaerobic and equol-producing bacterium, designated strain do03T, was isolated from the caecal content of a rat. Cells were Gram-positive, non-spore-forming rods. The results from a phylogenetic analysis based on 16S rRNA gene sequences showed that strain do03T formed a separate line of descent in the ph...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64894-0

    authors: Minamida K,Ota K,Nishimukai M,Tanaka M,Abe A,Sone T,Tomita F,Hara H,Asano K

    更新日期:2008-05-01 00:00:00

  • Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a.

    abstract::Among a group of marine isolates that were found to be positive for genes of the bacterial photosynthetic reaction centre, a strain was selected for characterization that was phylogenetically close to the genus Roseovarius. The strain, designated DFL-24T, originated from a culture of Alexandrium ostenfeldii (dinoflage...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63832-0

    authors: Biebl H,Allgaier M,Lünsdorf H,Pukall R,Tindall BJ,Wagner-Döbler I

    更新日期:2005-11-01 00:00:00

  • Sphingomonas populi sp. nov., isolated from bark of Populus × euramericana.

    abstract::One Gram-stain-negative, aerobic, motile bacterial strain, 3-7T, was isolated from symptomatic canker bark tissue of Populus × euramericana. 16S rRNA gene sequence data revealed that the novel isolate shared highest similarity with Sphingomonas panacis DCY99T (98.8 %), and <96.5 % sequence similarity with all other sp...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003841

    authors: Li Y,Bian DR,Chang JP,Guo LM,Yang XQ

    更新日期:2020-02-01 00:00:00

  • Limibaculum halophilum gen. nov., sp. nov., a new member of the family Rhodobacteraceae.

    abstract::A Gram-stain-negative, cream-pigmented, aerobic, non-motile, non-spore-forming and short-rod-shaped bacterial strain, designated CAU 1123T, was isolated from mud from reclaimed land. The strain's taxonomic position was investigated by using a polyphasic approach. Strain CAU 1123T grew optimally at 37 °C and at pH 7.5 ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002200

    authors: Shin YH,Kim JH,Suckhoom A,Kantachote D,Kim W

    更新日期:2017-10-01 00:00:00

  • Streptomyces ovatisporus sp. nov., isolated from deep marine sediment.

    abstract::The taxonomic position of a Gram-staining-positive strain, designated strain S4702T was isolated from a marine sediment collected from the southern Black Sea coast, Turkey, determined using a polyphasic approach. The isolate was found to have chemotaxonomic, morphological and phylogenetic properties consistent with it...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001442

    authors: Veyisoglu A,Cetin D,Inan Bektas K,Guven K,Sahin N

    更新日期:2016-11-01 00:00:00

  • Change of the names Haloanaerobiales, Haloanaerobiaceae and Haloanaerobium to Halanaerobiales, Halanaerobiaceae and Halanaerobium, respectively, and further nomenclatural changes within the order Halanaerobiales.

    abstract::A formal proposal is made to rename the order Haloanaerobiales as Halanaerobiales, the family Haloanaerobiaceae as Halanaerobiaceae, the genera Haloanaerobium and Haloanaerobacter as Halanaerobium and Halanaerobacter, respectively, and to change the specific epithets of Halanaerobium acetoethylicum, Halanaerobacter la...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-6-2229

    authors: Oren A

    更新日期:2000-11-01 00:00:00

  • Description of Conyzicola nivalis sp. nov., isolated from glacial snow, and emended description of the genus Conyzicola and Conyzicola lurida.

    abstract::A cold-tolerant, translucent, yellow-pigmented, Gram-stain-positive, non-motile, rod-shaped bacteria was isolated from snow of the Zadang Glacier on the Tibetan Plateau, PR China. 16S rRNA gene sequence similarity analysis indicated that the isolate was closely related to Conyzicola lurida KCTC 29231T and Leifsonia ps...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002027

    authors: Gu Z,Liu Y,Xu B,Wang N,Shen L,Liu H,Zhou Y,Xing T,Guo B,Liu X

    更新日期:2017-08-01 00:00:00

  • Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov.

    abstract::Five heterotrophic, aerobic, halotolerant and pigmented bacterial strains with gliding motility were isolated from Antarctic sea water; one other isolate was collected from the sea urchin Strongylocentrotus intermedius in the Gulf of Peter the Great in the Sea of Japan. 16S rRNA gene sequence analysis indicated that t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63410-0

    authors: Nedashkovskaya OI,Vancanneyt M,Dawyndt P,Engelbeen K,Vandemeulebroecke K,Cleenwerck I,Hoste B,Mergaert J,Tan TL,Frolova GM,Mikhailov VV,Swings J

    更新日期:2005-05-01 00:00:00

  • Desulfotomaculum defluvii sp. nov., a sulfate-reducing bacterium isolated from the subsurface environment of a landfill.

    abstract::A novel sulfate-reducing, strictly anaerobic and endospore-forming bacterium, designated strain A5LFS102(T), was isolated from a subsurface landfill sample. The strain was characterized using a polyphasic approach. Optimal growth was observed at 37 °C and pH 7.5 with sulfate as an electron acceptor. Sulfite and thiosu...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.047662-0

    authors: Krishnamurthi S,Spring S,Kumar PA,Mayilraj S,Klenk HP,Suresh K

    更新日期:2013-06-01 00:00:00

  • Gordonia malaquae sp. nov., isolated from sludge of a wastewater treatment plant.

    abstract::The taxonomic status of a bacterial isolate from the sludge of a wastewater treatment plant was characterized by using a polyphasic taxonomic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genu...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64893-0

    authors: Yassin AF,Shen FT,Hupfer H,Arun AB,Lai WA,Rekha PD,Young CC

    更新日期:2007-05-01 00:00:00

  • 'Candidatus Phytoplasma cirsii', a novel taxon from creeping thistle [Cirsium arvense (L.) Scop].

    abstract::Creeping thistle [Cirsium arvense (L.) Scop.] and dahlia (Dahlia sp.) plants showing typical symptoms of phytoplasma infection including yellowing, stunting, inflorescence and proliferation, were sampled; the presence of phytoplasma was confirmed by standard PCR using universal primers. RFLP analysis allowed classific...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000937

    authors: Šafárˇová D,Zemánek T,Válová P,Navrátil M

    更新日期:2016-04-01 00:00:00

  • Altererythrobacter sediminis sp. nov., isolated from lagoon sediments.

    abstract::A Gram-stain-negative, non-motile, non-spore-forming, ovoid rod-shaped bacterium, designated strain CAU1172T, was isolated from lagoon sediments along the east coast of the Republic of Korea. Strain CAU1172T formed a yellow pigment on marine agar. Growth occurred at 20-37 °C (optimum, 30 °C), at pH 6.5-10 (optimum, 7....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001535

    authors: Kim JH,Yoon JH,Kim W

    更新日期:2016-12-01 00:00:00

  • Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy.

    abstract::Several nocardioform actinomycetes were isolated from tufaceous surfaces with whitish-grey patinas in the catacombs of St Callistus in Rome, Italy. The morphology of the isolates and their chemotaxonomic characteristics such as LL-diaminopimelic acid in the cell-wall peptidoglycan, the major menaquinone of MK-9(H(4)),...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65613-0

    authors: Urzì C,De Leo F,Schumann P

    更新日期:2008-09-01 00:00:00

  • Natranaerobius thermophilus gen. nov., sp. nov., a halophilic, alkalithermophilic bacterium from soda lakes of the Wadi An Natrun, Egypt, and proposal of Natranaerobiaceae fam. nov. and Natranaerobiales ord. nov.

    abstract::Novel halophilic, alkalithermophilic, Gram-type-positive bacterial strains were isolated from sediment of alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Cells of strain JW/NM-WN-LFT were rod-shaped, non-spore-forming and non-motile. Strain JW/NM-WN-LFT grew (at pH55 degrees C 9.5) between 35 and 56 degrees ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65068-0

    authors: Mesbah NM,Hedrick DB,Peacock AD,Rohde M,Wiegel J

    更新日期:2007-11-01 00:00:00

  • Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil.

    abstract::A Gram-reaction-negative, rod-shaped marine bacterium, designated MEBiC08158(T), was isolated from sediments collected from Taean County, Korea, near the Hebei Spirit tanker oil spill accident. 16S rRNA gene sequence analysis revealed that strain MEBiC08158(T) was closely related to Alcanivorax marinus R8-12(T) (99.5%...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000244

    authors: Kyoung Kwon K,Hye Oh J,Yang SH,Seo HS,Lee JH

    更新日期:2015-07-01 00:00:00

  • Vibrio ouci sp. nov. and Vibrio aquaticus sp. nov., two marine bacteria isolated from the East China Sea.

    abstract::Two Gram-stain-negative, catalase- and oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains BEI176T and BEI207T, were isolated from seawater collected in the East China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains BEI176T and BEI207T belonged to ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003732

    authors: Li B,Li Y,Liu R,Xue C,Zhu X,Tian X,Wang X,Liang J,Zheng Y,Zhang XH

    更新日期:2020-01-01 00:00:00

  • Marinobacter maroccanus sp. nov., a moderately halophilic bacterium isolated from a saline soil.

    abstract::During the taxonomic investigation of exopolymer-producing halophilic bacteria, a rod-shaped, motile, Gram-stain-negative, halophilic bacterium, designated strain N4T, was isolated from a saline soil located in northern Morocco. Optimal growth of the isolate was at 30-37 ºC and at pH 7.0-8.0, in the presence of 5-7 % ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003134

    authors: Boujida N,Palau M,Charfi S,Manresa À,Skali Senhaji N,Abrini J,Miñana-Galbis D

    更新日期:2019-01-01 00:00:00

  • Notification that new names and new combinations have appeared in volume 54, part 3, of the IJSEM.

    abstract::This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein are listed acc...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63382-0

    authors:

    更新日期:2004-09-01 00:00:00

  • Microvirga lupini sp. nov., Microvirga lotononidis sp. nov. and Microvirga zambiensis sp. nov. are alphaproteobacterial root-nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts.

    abstract::Strains of Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes Listia angolensis (from Zambia) and Lupinus texensis (from Texas, USA). Phylogenetic analysis of the 16S rRNA gene showed that the novel strains belong to the genus Microvirga, with ≥ 96.1%...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.035097-0

    authors: Ardley JK,Parker MA,De Meyer SE,Trengove RD,O'Hara GW,Reeve WG,Yates RJ,Dilworth MJ,Willems A,Howieson JG

    更新日期:2012-11-01 00:00:00

  • Phylogenetic analysis of Saccharothrix and related taxa: proposal for Actinosynnemataceae fam. nov.

    abstract::Partial sequences for 16S rDNA were determined for strains of the genus Saccharothrix, including most described species, as well as strains of the described species of the related genera Kutzneria, Actinokineospora and Actinosynnema. These were aligned with published sequences for other species of these genera, as wel...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-1-331

    authors: Labeda DP,Kroppenstedt RM

    更新日期:2000-01-01 00:00:00

  • Halodesulfovibrio spirochaetisodalis gen. nov. sp. nov. and reclassification of four Desulfovibrio spp.

    abstract::An antibiotic-producing, obligate anaerobic, Gram-stain-negative, catalase- and oxidase-negative strain (JC271T) was isolated from a marine habitat and identified, based on 16S rRNA gene sequence analysis, as a novel member of the family Desulfovibrionaceae. The closest phylogenetic relatives of strain JC271T were fou...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001574

    authors: Shivani Y,Subhash Y,Sasikala C,Ramana CV

    更新日期:2017-01-01 00:00:00

  • Eubacterium maltosivorans sp. nov., a novel human intestinal acetogenic and butyrogenic bacterium with a versatile metabolism.

    abstract::A novel anaerobic, non-spore-forming bacterium was isolated from a faecal sample of a healthy adult. The isolate, designated strain YIT, was cultured in a basal liquid medium under a gas phase of H2/CO2 supplemented with yeast extract (0.1 g l-1). Cells of strain YIT were short rods (0.4-0.7×2.0-2.5 µm), appearing sin...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003028

    authors: Feng Y,Stams AJM,Sánchez-Andrea I,de Vos WM

    更新日期:2018-10-04 00:00:00

  • Genetic diversity of Agrobacterium species isolated from nodules of common bean and soybean in Brazil, Mexico, Ecuador and Mozambique, and description of the new species Agrobacterium fabacearum sp. nov.

    abstract::Agrobacterium strains are associated with soil, plants and animals, and known mainly by their pathogenicity. We studied 14 strains isolated from nodules of healthy soybean and common bean plants in Brazil, Mexico, Ecuador and Mozambique. Sequence analysis of the 16S rRNA gene positioned the strains as Agrobacterium, b...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004278

    authors: Delamuta JRM,Scherer AJ,Ribeiro RA,Hungria M

    更新日期:2020-07-01 00:00:00

  • Halovenus salina sp. nov., an extremely halophilic archaeon isolated from a saltern.

    abstract::An extremely halophilic archaeon was isolated from a water sample of Isla Bacuta saltern in Huelva, Spain. Strain ASP54(T) is a novel red-pigmented, motile, rod-shaped, Gram-stain-negative and strictly aerobic haloarchaeon. Strain ASP54(T) grew in media containing 15-30% (w/v) salts and optimally with 25% (w/v) salts....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000370

    authors: Infante-Domínguez C,Corral P,Sánchez-Porro C,Ventosa A

    更新日期:2015-09-01 00:00:00

  • Brevirhabdus pacifica gen. nov., sp. nov., isolated from deep-sea sediment in a hydrothermal vent field.

    abstract::A Gram-stain-negative, motile, aerobic bacterial strain, designated 22DY15T, was isolated from a deep-sea sediment sample collected from a hydrothermal vent field located in the East Pacific Rise. The isolate was a short rod with a single flagellum and was positive for catalase and oxidase activities. Q-10 was the pre...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000469

    authors: Wu YH,Xu L,Zhou P,Wang CS,Oren A,Xu XW

    更新日期:2015-10-01 00:00:00

  • Pedobacter ardleyensis sp. nov., isolated from soil in Antarctica.

    abstract::A red-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, strain R2-28T, was isolated from a soil sample collected from Ardley Island, South Shetland Islands, Antarctica, and was characterized taxonomically by using a polyphasic approach. The organism grew optimally at 18 °C in TSB. On the basis of phylo...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000504

    authors: Da X,Jiang F,Chang X,Ren L,Qiu X,Kan W,Zhang Y,Deng S,Fang C,Peng F

    更新日期:2015-11-01 00:00:00

  • Highly organized structure in the non-coding region of the psbA minicircle from clade C Symbiodinium.

    abstract::The chloroplast genes of dinoflagellates are distributed among small, circular dsDNA molecules termed minicircles. In this paper, we describe the structure of the non-coding region of the psbA minicircle from Symbiodinium: DNA sequence was obtained from five Symbiodinium strains obtained from four different coral host...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02594-0

    authors: Moore RB,Ferguson KM,Loh WK,Hoegh-Guldberg O,Carter DA

    更新日期:2003-11-01 00:00:00