Caspofungin reduces the incidence of fungal contamination in cell culture.

Abstract:

:Fungal contamination is a major problem in cell culture, and the antifungal compounds currently in use can affect cultured cells. Echinocandins are antifungal drugs that inhibit fungal cell wall synthesis by targeting an enzyme that has no counterpart in mammalian cells. We evaluated whether the echinocandin caspofungin affected the growth or morphology of six murine cell lines (a macrophage-like cell line (J774.16) and five hybridoma lines), or primary human endothelial cells. The antifungal did not influence cellular characteristics at concentrations less than 512 microg/ml, while effectively reducing the incidence of fungal contamination. Also, caspofungin did not affect the production of antibody by hybridoma cells, or alter the cytokine production of J774.16 cells, although modest increases in IL-4 and IFN-gamma occurred upon LPS stimulation. Hence, echinocandins appear to be relatively non-toxic, and protect against fungal contamination in cell culture.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Martinez LR,Ntiamoah P,Casadevall A,Nosanchuk JD

doi

10.1007/s11046-007-9063-2

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

279-86

issue

6

eissn

0301-486X

issn

1573-0832

journal_volume

164

pub_type

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