Abstract：
:In mammals ghrelin has a diverse range of effects including stimulation of gut motility but although present in teleost fish its effects on motility have not been investigated. The present study used bioinformatics to search for fish paralogues of the ghrelin receptor and the closely related motilin receptor, and investigated the effects of ghrelin and motilin on gut motility in zebrafish, Danio rerio. Fish paralogues of the human ghrelin and motilin receptor genes were identified, including those from the zebrafish. In addition, a third gene was identified in three species of pufferfish (the only fish genome completely sequenced), which is distinct from the ghrelin and motilin receptors but more closely aligned to these receptors relative to other G-protein coupled receptors. Immunohistochemistry demonstrated strong ghrelin receptor-like reactivity in the muscle of the zebrafish intestine. In isolated intestinal bulb and mid/distal intestine preparations, ghrelin, motilin, and the motilin receptor agonist erythromycin all evoked contraction; these responses ranged between 9% and 51% of the contractions evoked by carbachol (10(-6) M). There were some variations in the concentrations found to be active in the different tissues, e.g., whereas motilin and rat ghrelin caused contraction of the intestinal bulb circular muscle at concentrations as low as 10(-8) M, human ghrelin (10(-8) to 10(-6) M) was without activity. Neither ghrelin (10(-7) M) nor erythromycin (10(-5) M) affected the contractions evoked by electrical field stimulation. The results suggest that both ghrelin and motilin can regulate intestinal motility in zebrafish and most likely other teleosts, and are discussed in relation to the evolution of these regulatory peptides.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Olsson C,Holbrook JD,Bompadre G,Jönsson E,Hoyle CH,Sanger GJ,Holmgren S,Andrews PLdoi
10.1016/j.ygcen.2007.05.016subject
Has Abstractpub_date
2008-01-01 00:00:00pages
217-26issue
1eissn
0016-6480issn
1095-6840pii
S0016-6480(07)00176-1journal_volume
155pub_type
杂志文章abstract：:In many fishes, dopamine (DA) strongly inhibits luteinizing hormone (LH) release by direct action at the pituitary level. In this study, the effect of DA on LH synthesis was examined by detecting its β-subunit mRNA level in immature grass carp pituitary cells. Results showed that DA inhibited LHβ mRNA expression and i...
journal_title：General and comparative endocrinology
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abstract：:Insulin (INS) plays a critical role in the growth, development, and metabolism of vertebrates. In this study, a cDNA encoding a novel insulin receptor (IR) subtype was isolated, cloned, and sequenced from the liver of rainbow trout. A 1525-bp cDNA encoding a partial amino acid sequence of the β-subunit including the t...
journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7687
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1050
更新日期：1993-03-01 00:00:00
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journal_title：General and comparative endocrinology
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更新日期：2008-11-01 00:00:00
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1089
更新日期：1993-06-01 00:00:00
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journal_title：General and comparative endocrinology
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更新日期：1986-04-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1152
更新日期：1993-11-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90106-0
更新日期：1984-08-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90037-8
更新日期：1988-03-01 00:00:00
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.10.008
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
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journal_title：General and comparative endocrinology
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更新日期：2016-03-01 00:00:00
abstract：:Relaxin 3 (RLN3) is a newly-discovered member of the insulin superfamily. We isolated three RLN3-like cDNAs from the brain of the Japanese eel (Anguilla japonica). The deduced amino acid sequences of the RLN3-like cDNAs contained the two-chain structure common to relaxin including a RXXXRXXI/V motif in the B-chain. Ph...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
abstract：:The penultimate instar larvae of Mamestra brassicae have one ecdysteroid peak 2 days after ecdysis which induces larval ecdysis. Allatectomy deleted this peak and caused a larger peak 7 days later just before precocious pupation, in a pattern similar to the ecdysteroid titer of the last instar. Application of juvenile...
journal_title：General and comparative endocrinology
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