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The GLP large scale preparation of immunotoxins containing deglycosylated ricin A chain and a hindered disulfide bond.

Abstract：

:The large scale preparation of two second generation immunotoxins containing murine monoclonal antibodies and deglycosylated ricin A chain is described. The procedure for the preparation of immunotoxins consists of the derivatization of antibody with SMPT and reduction of dgA with DTT followed by their reaction to establish a hindered interchain disulfide bond. The purification of the immunotoxin includes affinity chromatography on Blue-Sepharose to remove the free antibody and gel filtration on Sephacryl S-200HR to remove any high molecular weight material and free dgA. The two immunotoxins were prepared by GLP procedures and tested for yield, composition, purity, sterility and biological activity.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Ghetie V,Thorpe P,Ghetie MA,Knowles P,Uhr JW,Vitetta ES

doi

10.1016/0022-1759(91)90110-2

subject

Has Abstract

pub_date

1991-09-13 00:00:00

pages

223-30

issue

2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(91)90110-2

journal_volume

142

pub_type

杂志文章

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