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Injection of nuclear factor-kappa B decoy into the sciatic nerve suppresses mechanical allodynia and thermal hyperalgesia in a rat inflammatory pain model.

Abstract：

STUDY DESIGN:In vitro and in vivo study of a rat inflammatory pain model using nuclear factor-kappa B decoy. OBJECTIVES:To investigate transduction efficiency of nuclear factor-kappa B decoy into dorsal root ganglion, both in vivo and in vitro, and to assess the suppression of inflammatory pain by nuclear factor-kappa B decoy. SUMMARY OF BACKGROUND DATA:Transcription factor nuclear factor-kappa B is reported to play a crucial role in regulating pro-inflammatory cytokine gene expression. We hypothesized that inhibiting nuclear factor-kappa B gene expression with nuclear factor-kappa B decoy may suppress inflammatory pain. METHODS:Nuclear factor-kappa B decoy-fluorescein isothiocyanate (FITC) was induced in explant culture, endoneurally injected into the sciatic nerve, and its transduction efficiency into dorsal root ganglion measured. For behavioral testing, 12 rats received plantar injections of complete Freund's adjuvant and were divided into 3 groups: decoy group, single endoneural injection of 10 microL of nuclear factor-kappa B decoy (n = 4); saline group, single endoneural injection of 10 microL of saline (n = 4); and naïve group, untreated (n = 4). Behavioral testing was performed using von Frey filaments and a Hargreaves device with a heat source. RESULTS:Total transduction efficiency of nuclear factor-kappa B decoy-FITC was 53.6% in vitro and 20.5% in vivo. No statistical differences were observed with respect to types of cell size distributions of all FITC-positive neurons. In behavioral testing, withdrawal latencies or thresholds significantly differed between the decoy group and the saline group from 2 to 14 days after surgery in the mechanical allodynia experiments, and from 2 to 3 days after surgery in the thermal hyperalgesia experiments. CONCLUSIONS:Nuclear factor-kappa B decoy was conveyed and transduced into dorsal root ganglion both in vivo and in vitro. Additionally, nuclear factor-kappa B decoy reduced mechanical allodynia and thermal hyperalgesia in the rat inflammatory pain model, suggesting that inhibition of nuclear factor-kappa B with nuclear factor-kappa B decoy may represent a key mechanism for mediating inflammation or reducing inflammatory pain.

journal_name

Spine (Phila Pa 1976)

journal_title

Spine

authors

Inoue G,Ochiai N,Ohtori S,Nakagawa K,Gemba T,Doya H,Ito T,Koshi T,Moriya H,Takahashi K

doi

10.1097/01.brs.0000248424.46652.67

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

2904-8

issue

eissn

0362-2436

issn

1528-1159

pii

00007632-200612010-00005

journal_volume

pub_type

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Injection of nuclear factor-kappa B decoy into the sciatic nerve suppresses mechanical allodynia and thermal hyperalgesia in a rat inflammatory pain model.