Abstract:
:A detailed characterization of two humic fractions was performed: One with low relative molecular mass (LMr<3,500 Da) and one with high relative molecular mass (HMr>3,500 Da). Distinct (1)H NMR spectroscopic patterns were observed for the two fractions. HMr showed an aromatic proton region, an intense and broad region (3.0-5.0 ppm) attributed to sugar-like and polyether components, and an intense doublet at 1.33 ppm (identified as protons of the beta-CH(3) in lactate). In contrast, LMr did not show resonances due to aromatic protons and was characterized by a broad unresolved region, assigned to sugar-like components. The (13)C NMR spectra showed that the LMr humic fraction was richer in carboxylic and aliphatic C groups compared to HMr fraction. These substances were fluorescein-labeled [fluorescein isothiocyanate (FITC)], and their interaction with carrot cells in culture was monitored for 10 d, and compared to FITC-indole-3-acetic acid (IAA) to clarify their mechanisms of biological activity. After different incubation times, fluorescein staining of carrot cells and decrease of fluorescein concentration in the culture medium were evaluated. Fluorescent membrane staining was only present in IAA and the LMr humic fraction treated cell cultures. A consequential decrease of fluorescein concentration in the culture media was also observed. Pretreatment of carrot cells with unconjugated IAA or LMr humic fraction markedly reduced fluorescein staining of both FITC-IAA and FITC-LMr humic fraction. Blocking tests gave indirect evidence of possible binding of the LMr humic fraction to IAA cell membrane receptors. These results indicate that the two humic fractions behave differently. Only LMr humic fraction, like IAA, interacts with cellular membranes in carrot cell cultures.
journal_name
J Chem Ecoljournal_title
Journal of chemical ecologyauthors
Muscolo A,Sidari M,Francioso O,Tugnoli V,Nardi Sdoi
10.1007/s10886-006-9206-9subject
Has Abstractpub_date
2007-01-01 00:00:00pages
115-29issue
1eissn
0098-0331issn
1573-1561journal_volume
33pub_type
杂志文章abstract::The sequence of arrival of the bark beetlesIps typographus andPityogenes chalcographus (Coleoptera: Scolytidae) at traps baited with their synthetic pheromones was monitored with a portable fraction collector. Histograms of the natural arrival patterns of both species were nonrandom and clumped at shorter time scales ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02040099
更新日期:1996-11-01 00:00:00
abstract::Information processing in the olfactory pathway underlying the perception of semiochemicals by insects is discussed. Both the chemical message for mates and the message for food consist of blends of chemicals. Olfactory receptors in an insect species are tuned to the detection of those compounds which comprise such ch...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01014246
更新日期:1988-11-01 00:00:00
abstract::TheMaladera matrida beetle (Coleoptera, Scarabaeidae, Melolonthinae), a relatively new species to science, was first identified in Israel in 1983. In the course of field observations it was found that adultM. matrida beetles emerged from the soil at sunset to feed and mate. During the first 20 min of flight, most of t...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02036200
更新日期:1994-10-01 00:00:00
abstract::Extracts of Amphimallon solstitiale (L.), a well known, widely distributed and rather common European scarab beetle, were analyzed by GC-MS and GC-EAD. Acetoin - (R):(S) > 9:1 - as well as 2,3-butanediol - (2R,3R): (2S,3S):meso = 1:1:9 - were present in extracts of both males and females. Although (2S,3S)-butanediol d...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/a:1022992516854
更新日期:2003-04-01 00:00:00
abstract::The salticid spider Cosmophasis bitaeniata preys on the larvae of the green tree ant Oecophylla smaragdina. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) reveal that the cuticle of C. bitaeniata mimics the mono- and dimethylalkanes of the cuticle of its prey. Recognition bioassays with extra...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/a:1015249012493
更新日期:2002-04-01 00:00:00
abstract::Conifers possess a suite of physiochemical defenses that protect their subcortical tissues from bark beetle - fungal complexes. These defenses include rapid induction of terpenoids and phenolics at the site of attack. Studies of the distribution, induction, and bioactivity of conifer terpenoids have focused heavily on...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-015-0588-4
更新日期:2015-06-01 00:00:00
abstract::Hard clams, Mercenaria mercenaria, are sessile, filter-feeding organisms that are heavily preyed upon by blue crabs, which find their clam prey using chemical cues. Clams may evade blue crabs by reducing their pumping (feeding) behavior when a threat is perceived. The purpose of this study was to determine the type of...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-005-9021-8
更新日期:2006-03-01 00:00:00
abstract::Several plants and invertebrates interact and communicate by means of volatile organic compounds (VOCs). These compounds may play the role of infochemicals, being able to carry complex information to selected species, thus mediating inter- or intra-specific communications. Volatile organic compounds derived from the w...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-015-0610-x
更新日期:2015-08-01 00:00:00
abstract::Feeding of theophylline to hornet workers from their moment of eclosion during the summer results in the following effects on the hornets: loss of appetite for proteins, inhibited ovarial development, no cell-building activity, negative phototaxis, gradual slow-down of spontaneous motor activity, difficulty in flight ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00984011
更新日期:1982-01-01 00:00:00
abstract::Cuticular aphrodisiacs fromD. melanogaster females were further characterized and the male response specificity towards such natural and synthetic unsaturated hydrocarbons was investigated. The behavioral activity seems to be correlated with some chain-length requirement and double-bond position; at least one double b...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01012116
更新日期:1985-12-01 00:00:00
abstract::Twenty ent-labdane diterpenes, isolated from the aquatic plants Ruppia maritima and Potamogeton natans, were tested to detect their effects on aquatic organisms from different trophic levels. Toxicity tests were performed on aquatic producers (the alga Selenastrum capricornutum), and consumers including a rotifer (Bra...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/a:1016213630957
更新日期:2002-06-01 00:00:00
abstract::Volatile monoterpenes such as 1,8-cineole inhibit the growth of Brassica campestris seedlings in a dose-dependent manner, and the growth-inhibitory effects are more severe for roots than hypocotyls. The preferential inhibition of root growth may be explained if the compounds inhibit cell proliferation more severely th...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-011-9919-2
更新日期:2011-03-01 00:00:00
abstract::Previous analyses of the scent gland secretions of snakes have focused on the nonvolatile components. Gas chromatography-mass spectrometry of secretions from North American garter snakes (Thamnophis butleri, T. couchi, T. elegans, T. melanogaster, andT. sirtalis) indicated the following seven major volatile components...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02036652
更新日期:1995-02-01 00:00:00
abstract::The chemical compositions of anal sac secretions of seven mustelid species were examined by thin-layer chromatography, gas chromatography, and mass spectrometry. The analyses showed great similarities between species belonging to the genusMustela, i.e.,M. erminea,M. nivalis,M. vison, andM. putorius, whereasMaries mart...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00988779
更新日期:1983-06-01 00:00:00
abstract::The attractiveness of different synthetic host odors and a synthetic aggregation pheromone (grandisoic acid [GA]) to overwintered adult plum curculios (PCs), Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), was examined using two types of traps (sticky panels and black pyramids) placed in border areas surr...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/b:joec.0000008017.16911.aa
更新日期:2003-12-01 00:00:00
abstract::Cyclohexanone, a naturally occurring component of male Asian elephant temporal gland secretion, was tested as a candidate elicitor of bioresponses from female Asian elephants (Elephas maximus). Four female Asian elephants were presented with synthetic cyclohexanone samples during a standardized bioassay. Four types of...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02098394
更新日期:1994-11-01 00:00:00
abstract::Crude extracts of root of the forage legumesLotus pedunculatus andCoronilla varia (crownvetch) were toxic when administered orally to 3rd instarCostelytra zealandica larvae. A group of 3-nitropropanoyl-D-glucopyranoses was isolated from active fractions of the crude extracts. These toxins, some of which were already k...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00987645
更新日期:1984-01-01 00:00:00
abstract::Most studies on rhizosphere chemicals are carried out in substrate-free set-ups or in artificial substrates using sampling methods that require an air flow and may thus cause disturbance to the rhizosphere. Our study aimed to develop a simplified and inexpensive system that allows analysis of rhizosphere chemicals at ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-015-0559-9
更新日期:2015-03-01 00:00:00
abstract::Olfactory responses of 72- to 96-hr-old, 24-hr starved adult male, female, or mixed-sex groups of the red flour beetle,Tribolium castaneum (Herbst), to volatiles from wheat and millet kernels, certain milled fractions, and solvent extracts were recorded by using a lightsensitive apparatus. Wheat-germ volatiles from in...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00989103
更新日期:1982-12-01 00:00:00
abstract::Several unsaturated aldehydes are produced from polyunsaturated fatty acids via the lipoxygenase pathway when soybean (Glycine max) plants are wounded mechanically or by pathogens. The effects of four of these aldehydes were examined on the growth of isolated fungal cultures ofColletotrichum truncatum, Rhizoctonia sol...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00979668
更新日期:1993-10-01 00:00:00
abstract::The flower bug Orius sauteri is a generalist predator that occurs throughout Japan, and is a promising indigenous natural enemy for micro-pests such as thrips, aphids, and spider mites. We aimed to manipulate the attraction, dispersal, and settlement behavior of Orius bugs using natural chemical substances emitted by ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-019-01104-1
更新日期:2019-10-01 00:00:00
abstract::Jasmonic acid (JA) is a key molecule initiating plant defensive responses to attack by pathogens and herbivores. This phytohormone is produced at sites of insect damage and is ingested by feeding insects, but its subsequent occurrence in insect tissues remains to be studied. We report the presence of JA in eggs and ne...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-005-8553-2
更新日期:2005-11-01 00:00:00
abstract::The cuticular lipid composition of lower and upper leaves of five genotypes of field-grown corn,Zea mays L., was determined by combined gas chromatography-mass spectrometry. Surface lipids of the upper leaves had a higher proportion ofn-alkanes (45-52%) than the lower leaves, while the lower leaves had higher percenta...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00983808
更新日期:1993-09-01 00:00:00
abstract::The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-μl aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad Bradford dye reagen...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01015193
更新日期:1989-03-01 00:00:00
abstract::In order to test a previous conclusion that chain-elongated analogs of (Z)-5-decenyl acetate(1), a pheromone component of the turnip moth,Agrotis segetum, adopt a loop conformation of the terminal alkyl chain in the bioactive conformation, a series of alkyl ether and enol ether analogs of1 and (Z)-5-dodecenyl acetate(...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02033463
更新日期:1995-06-01 00:00:00
abstract::The symbiotic fungus Amylostereum areolatum is essential for growth and development of larvae of the invasive woodwasp, Sirex noctilio. In the nutrient poor xylem of pine trees, upon which Sirex feeds, it is unknown whether Amylostereum facilitates survival directly through consumption (mycetophagy) and/or indirectly ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-012-0222-7
更新日期:2013-01-01 00:00:00
abstract::The major component of the rectal glandular extract and volatile emission of maleBactrocera cacuminatus is racemic 1,7-dioxaspiro[5.5]undecane. l-Hydroxy-5-nonanone as its open chain form, together with 6-n-butyl-3,4-dihydro-2H-pyran are minor components. 1,7-Dioxaspiro[5.5]undecan-4-ol is present at a low level and i...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00994347
更新日期:1991-02-01 00:00:00
abstract::The outcome of plant-mediated interactions among herbivores from several feeding guilds has been studied intensively. However, our understanding on the effects of nematode root herbivory on leaf miner oviposition behavior and performance remain limited. In this study, we evaluated whether Meloidogyne incognita root he...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-016-0810-z
更新日期:2017-02-01 00:00:00
abstract::We characterized the variation in cuticular hydrocarbon mixtures between seven colonies of the Formosan subterranean termite,Coptotermes formosanus Shiraki, from the same population. We report differences between castes, between colonies, and within the population over time to assess seasonality. Colonies ofC. formosa...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02028506
更新日期:1996-10-01 00:00:00
abstract::Age-related changes of antennal-active components of male labial gland extracts were studied in two closely related bumblebee species, Bombus terrestris and B. lucorum. In B. terrestris, compounds eliciting electroantennogram (EAG) responses of virgin queens were ethyl dodecanoate, 2,3-dihydrofarnesal, 2,3-dihydrofarn...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-009-9650-4
更新日期:2009-06-01 00:00:00