Measuring plant protein with the Bradford assay : 1. Evaluation and standard method.

Abstract:

:The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-μl aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad Bradford dye reagent (Coomassie brilliant blue G-250) diluted 1:4 and containing 3 mg/ml soluble polyvinylpyrollidone. Absorbance at 595 nm is recorded after 15 min against an NaOH blank. Samples are calibrated against a ribulose 1,5-diphosphate carboxylase-oxygenase standard in NaOH. Procedures for plant preparation, extraction stability, the effects of phenol removal and quinone formation, and assay recovery are evaluated. Assay absorbance stability and techniques for increasing absorbance stability are reported. Changes in protein quality are briefly discussed.

journal_name

J Chem Ecol

authors

Jones CG,Daniel Hare J,Compton SJ

doi

10.1007/BF01015193

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

979-92

issue

3

eissn

0098-0331

issn

1573-1561

journal_volume

15

pub_type

杂志文章
  • Sexual dimorphism in territorial scent marking by adult Eurasian beavers (Castor fiber).

    abstract::Mammalian scent marking is often associated with territorial defense. However, males and females may demonstrate different activity patterns and play different roles. Female mammals nurture the young during lactation, while males purportedly perform other tasks more frequently, such as territorial maintenance and defe...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-006-9087-y

    authors: Rosell F,Thomsen LR

    更新日期:2006-06-01 00:00:00

  • Olfactory sensitivity of two sympatric species of rice leaf folders (Lepidoptera: Pyralidae) to plant volatiles.

    abstract::Electroantennograms (EAGs) were recorded from male and female moths of two sympatric leaf folder species,Cnaphalocrocis medinalis (Gue-née) andMarasmia patnalis Bradley (Lepidoptera: Pyralidae) to 91 volatile chemicals of plant origin. Responses of both leaf folder species were similar to all compounds except to three...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00988076

    authors: Ramachandran R,Khan ZR,Caballero P,Juliano BO

    更新日期:1990-09-01 00:00:00

  • Reproductive performance and population dynamics of cowpea aphid (Homoptera: Aphididae) on leaf extracts of resistant and susceptible cowpeas.

    abstract::Membrane feeding studies were conducted to determine the effects of raw juices and chemical extracts of leaves of aphid-resistant (ICV-12) and aphid-susceptible (ICV-1) cultivars of cowpea [Vigna unguiculata L. (Walp.)], on the survival, growth, and reproduction of cowpea aphidAphis craccivora Koch. Life table and dem...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF02266971

    authors: Annan IB,Tingey WM,Schaefers GA,Saxena KN

    更新日期:1996-07-01 00:00:00

  • Separation and identification of phenolic acids and related compounds by gas chromatography and fourier transform infrared spectroscopy.

    abstract::Phenolic acids and related compounds were separated by gas chromatography using three separate columns. One of these columns was coupled to a Fourier transform infrared spectrometer. The trimethylsilyl derivatives could be separated and identified by comparing the relative retention times of the three different column...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF01025891

    authors: Melhuish JH Jr,Willis RB,Wright CS

    更新日期:1987-02-01 00:00:00

  • Response of the clerid predatorThanasimus dubius (F.) to bark beetle pheromones and tree volatiles in a wind tunnel.

    abstract::Tree volatiles and pheromones produced by southern bark beetles were bioassayed for response by the clerid predatorThanasimus dubius (F.). Upwind flights in a laboratory olfactometer, modified from Visser (1976), were used to determine the attractiveness of compounds. Differences in response to a solvent control and p...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00987655

    authors: Mizell RF 3rd,Frazier JL,Nebeker TE

    更新日期:1984-01-01 00:00:00

  • Acceptance threshold hypothesis is supported by chemical similarity of cuticular hydrocarbons in a stingless bee, Melipona asilvai.

    abstract::The ability to discriminate nestmates from non-nestmates in insect societies is essential to protect colonies from conspecific invaders. The acceptance threshold hypothesis predicts that organisms whose recognition systems classify recipients without errors should optimize the balance between acceptance and rejection....

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-012-0194-7

    authors: Nascimento DL,Nascimento FS

    更新日期:2012-11-01 00:00:00

  • Chemistry of Glossodoris nudibranchs: specific occurrence of 12-keto scalaranes.

    abstract::With the aim of establishing whether the oxidation of the tricyclic carbon skeleton of scalaradial (1) is specific to Glossodoris mollusks, the chemical investigation of specimens of Glossodoris pallida from two distinct geographical areas (China and Guam) and of Glossodoris vespa and Glossodoris averni from Eastern A...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-007-9387-x

    authors: Manzo E,Gavagnin M,Somerville MJ,Mao SC,Ciavatta ML,Mollo E,Schupp PJ,Garson MJ,Guo YW,Cimino G

    更新日期:2007-12-01 00:00:00

  • (+)-cis-3-Pinen-2-ol: Attractant for male cerambycid beetle,Monochamus alternatus hope.

    abstract::A monoterpene alcohol was isolated from paraquat-induced lightwood in pines and identified as (+)-cis-3-pinen-2-ol [(+)-3]. More than a certain amount of (+)-3 induced a laboratory flight response by the male cerambycid beetle,Monochamus altematus Hope, but (+)-3 stimulated no response by the female at any dose level ...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00994198

    authors: Sakai M,Toruyamasaki T

    更新日期:1991-04-01 00:00:00

  • Kairomone strains of Euclytiaflava (Townsend), a parasitoid of stink bugs.

    abstract::Tachinid flies commonly use the pheromones and allomones of stink bugs (Pentatomidae) as host-finding kairomones. Pheromone-baited traps for predaceous (Podisus spp.) and phytophagous (Euschistus spp.) pentatomids were used to obtain tachinid parasitoids in order to study the semiochemical relationships between these ...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1023/a:1019972312132

    authors: Aldrich JR,Zhang A

    更新日期:2002-08-01 00:00:00

  • Development and evaluation of systems to collect volatile semiochemicals from insects and plants using a charcoal-infused medium for air purification.

    abstract::A system is described for the collection of volatiles produced by plants and insects that minimizes stresses on the plant or insect in an environment that is free from chemical impurities. Air entering a volatile collection chamber containing insects and/or plants was purified using a nonwoven fabric medium infused wi...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00980075

    authors: Heath RR,Manukian A

    更新日期:1992-07-01 00:00:00

  • Nerol: An alarm substance of the stingless bee,Trigona fulviventris (Hymenoptera: Apidae).

    abstract::Bees of the genusTrigona and subgenusTrigona possess volatile materials in their mandibular glands, used as alarm substances and as marking pheromones. Heads of workers ofTrigona fulviventris were analyzed by gas chromatography-mass spectrometry. The two major volatile components were nerol (∼ 50%), and octyl caproate...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00990750

    authors: Johnson LK,Wiemer DF

    更新日期:1982-09-01 00:00:00

  • Attractive and inhibitory pheromones produced in the bark beetle,Dendroctonus brevicomis, during host colonization: Regulation of inter- and intraspecific competition.

    abstract::Quantities of attractive (exo-brevicomin and frontalin) and inhibitory (trans-verbenol, verbenone, and ipsdienol) pheromones were monitored in both sexes ofDendroctonus brevicomis during their colonization of a ponderosa pine. Verbenone was found in males in the greatest amounts at the time of landing, and it declined...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00987969

    authors: Byers JA,Wood DL,Craig J,Hendry LB

    更新日期:1984-06-01 00:00:00

  • Food experience on the predatory behavior of the ant Myrmica rubra towards a specialist moth, Acrolepiopsis assectella.

    abstract::Entomophagous insects are often repelled by the secondary compounds of the plants eaten by their prey. These compounds, therefore, take on a defensive role for the phytophagous species that sequester them. Given that numerous entomophagous species are capable of learning, the effects on the foraging behavior of a repe...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1023/a:1021009618298

    authors: Le Roux AM,Le Roux G,Thibout E

    更新日期:2002-11-01 00:00:00

  • Defense of parsnip webworm against phototoxic furanocoumarins: Role of antioxidant enzymes.

    abstract::The parsnip webworm,Depressaria pastinacella (Lepidoptera: Oecophoridae), feeds on plants rich in furanocoumarins, phototoxic allomones. Final-instar larvae possess high levels of activities of antioxidant enzymes (Superoxide dismutase, catalase, glutathione reductase), which detoxify oxygen radicals generated from th...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF01017468

    authors: Lee K,Berenbaum MR

    更新日期:1990-08-01 00:00:00

  • Byrsonic acid--the clue to floral mimicry involving oil-producing flowers and oil-collecting bees.

    abstract::Tetrapedia diversipes and other Apidae (Anthophoridae) may be deceived by floral similarities between Malpighiaceae and Orchidaceae of the Oncidiinae subtribe. The latter do not usually exudate floral oils. Thus, visitors may pollinate the flowers in a deceit/food/pollination syndrome. We studied the chemical composit...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-007-9309-y

    authors: Reis MG,de Faria DA,dos Santos IA,Amaral Mdo C,Marsaioli AJ

    更新日期:2007-07-01 00:00:00

  • The Origin and Ecological Function of an Ion Inducing Anti-Predator Behavior in Lithobates Tadpoles.

    abstract::In aquatic environments, chemical cues are believed to be associated with prey response to predation risk, yet few basic cue compositions are known despite the pronounced ecological and evolutionary significance of such cues. Previous work indicated that negatively-charged ions of m/z 501 are possibly a kairomone that...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-018-0925-5

    authors: Austin CE,March RE,Stock NL,Murray DL

    更新日期:2018-02-01 00:00:00

  • Specificity of herbivore-induced hormonal signaling and defensive traits in five closely related milkweeds (Asclepias spp.).

    abstract::Despite the recognition that phytohormonal signaling mediates induced responses to herbivory, we still have little understanding of how such signaling varies among closely related species and may generate herbivore-specific induced responses. We studied closely related milkweeds (Asclepias) to link: 1) plant damage by...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-014-0449-6

    authors: Agrawal AA,Hastings AP,Patrick ET,Knight AC

    更新日期:2014-07-01 00:00:00

  • Impact of elevated levels of atmospheric CO2 and herbivory on flavonoids of soybean (Glycine max Linnaeus).

    abstract::Atmospheric levels of carbon dioxide (CO2) have been increasing steadily over the last century. Plants grown under elevated CO2 conditions experience physiological changes, particularly in phytochemical content, that can influence their suitability as food for insects. Flavonoids are important plant defense compounds ...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-009-9727-0

    authors: O'Neill BF,Zangerl AR,Dermody O,Bilgin DD,Casteel CL,Zavala JA,DeLucia EH,Berenbaum MR

    更新日期:2010-01-01 00:00:00

  • Inactivation and mutagenesis by phototoxins usingEscherichia coli strains differing in sensitivity to near- and far-ultraviolet light.

    abstract::FourEscherichia coli strains carrying all the possible combinations of genes controlling sensitivity to near-UV (NUV;nur versusnur (+)) and far-UV (FUV;uvrA6 versusuvrA (+)) were inactivated with broad-spectrum NUV together with specific phototoxins. The inactivation kinetics of the four strains are consistent with th...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF01020262

    authors: Tuveson RW,Berenbaum MR,Heininger EE

    更新日期:1986-04-01 00:00:00

  • Lipoxygenase-derived aldehydes inhibit fungi pathogenic on soybean.

    abstract::Several unsaturated aldehydes are produced from polyunsaturated fatty acids via the lipoxygenase pathway when soybean (Glycine max) plants are wounded mechanically or by pathogens. The effects of four of these aldehydes were examined on the growth of isolated fungal cultures ofColletotrichum truncatum, Rhizoctonia sol...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00979668

    authors: Vaughn SF,Gardner HW

    更新日期:1993-10-01 00:00:00

  • Identification of female-produced sex pheromone from banded cucumber beetle,Diabrotica balteata leconte (Coleoptera: Chrysomelidae).

    abstract::A sex pheromone produced by female banded cucumber beetle adults,Diabrotica balteata LeConte, was isolated from volatiles trapped on Porapak Q and identified as 6,12-dimethylpentadecan-2-one. The structure was elucidated by spectroscopic analyses and confirmed by synthesis. The synthesized racemic compound was equal t...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF00980203

    authors: Chuman T,Guss PL,Doolittle RE,McLaughlin JR,Krysan JL,Schalk JM,Tumlinson JH

    更新日期:1987-07-01 00:00:00

  • Male and female noctuid moths attracted to synthetic lures in Europe.

    abstract::In field tests in Europe, traps baited with a blend of isoamyl alcohol, acetic acid, and isobutanol (compounds previously found attractive to a number of noctuids in North America) caught the following noctuid moths: Agrotis segetum Schiff., Agrotis crassa Hbn., Agrotis exclamationis L., Amathes (Xestia) c-nigrum L., ...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-010-9789-z

    authors: Tóth M,Szarukán I,Dorogi B,Gulyás A,Nagy P,Rozgonyi Z

    更新日期:2010-06-01 00:00:00

  • The Male Produced Aggregation Pheromone of a Strawberry Sap Beetle, Lobiopa insularis (Coleoptera: Nitidulidae).

    abstract::The nitidulid beetle Lobiopa insularis is an important pest of strawberry crops in the United States and Brazil. Both larvae and adults feed on ripe strawberries, causing 20-70% loss in production during serious infestations. Aiming at the development of efficient, clean, and highly specific pest management systems, s...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-017-0851-y

    authors: Moliterno AAC,Martins CBC,Szczerbowski D,Zawadneak MAC,Zarbin PHG

    更新日期:2017-06-01 00:00:00

  • Testing the effects of drying methods on willow flavonoids, tannins, and salicylates.

    abstract::In this study, we compared the effects of several preservation methods on the secondary phenolics of the mature leaves of purple willow (Salix purpurea L., salicaceae) with results obtained with fresh leaf analyses. Conventional freeze-drying, in which the leaves were first frozen with liquid nitrogen and then placed ...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1023/a:1010358120482

    authors: Julkunen-Tiitto R,Sorsa S

    更新日期:2001-04-01 00:00:00

  • Protected by fumigants: beetle perfumes in antimicrobial defense.

    abstract::Beetles share with other eukaryotes an innate immune system that mediates endogenous defense against pathogens. In addition, larvae of some taxa produce fluid exocrine secretions that contain antimicrobial compounds. In this paper, we provide evidence that larvae of the brassy willow leaf beetle Phratora vitellinae co...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-007-9416-9

    authors: Gross J,Schumacher K,Schmidtberg H,Vilcinskas A

    更新日期:2008-02-01 00:00:00

  • Plant growth regulatory activities of artemisinin and its related compounds.

    abstract::Artemisinin, a sesquiterpene lactone produced in the leaves ofArtemisia annua, was evaluated for its phytotoxicity in mono- and dicotyledonous plants. Artemisinin inhibited seed germination, seedling growth, and root induction in all species tested. The concentration of artemisinin required for 50% inhibition ofLemna ...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/BF01020500

    authors: Chen PK,Leather GR

    更新日期:1990-06-01 00:00:00

  • Salivary proteins as a defense against dietary tannins.

    abstract::Tannins, a diverse group of water-soluble phenolics with high affinity to proteins, are widely distributed in various parts of plants, and have negative effects in herbivores after ingestion. Some mammalian species are thought to counteract tannins by secreting tannin-binding salivary proteins (TBSPs). Several types o...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章,评审

    doi:10.1007/s10886-006-9077-0

    authors: Shimada T

    更新日期:2006-06-01 00:00:00

  • Fruit Odor as A Ripeness Signal for Seed-Dispersing Primates? A Case Study on Four Neotropical Plant Species.

    abstract::Fleshy fruits contain a myriad of secondary metabolites that may fulfill various non-mutually exclusive ecological functions. Among them are defense against pathogens and herbivores, manipulation of frugivores' gut retention time, or controlling the germination process. In addition, it has been suggested that aroma co...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-016-0687-x

    authors: Nevo O,Heymann EW,Schulz S,Ayasse M

    更新日期:2016-04-01 00:00:00

  • The involvement of volatile infochemicals from spider mites and from food-plants in prey location of the generalist predatory mite Neoseiulus californicus.

    abstract::We investigated volatile infochemicals possibly involved in location of the generalist predatory mite Neoseiulus californicus to plants infested with spider mites in a Y-tube olfactometer. The predators significantly preferred volatiles from lima bean leaves infested with Tetranychus urticae to uninfested lima bean le...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-005-6075-6

    authors: Shimoda T,Ozawa R,Sano K,Yano E,Takabayashi J

    更新日期:2005-09-01 00:00:00

  • The effect of sampling methods on the apparent constituents of ink from the squid Sepioteuthis australis.

    abstract::Results of experiments conducted on ink recovered from the squid Sepioteuthis australis indicate that there is no epinephrine or protein naturally present in the ink as it would be ejected in vivo. Protein content was effectively zero when ink was syringed from the duct end of the ink sac of freshly killed animals. By...

    journal_title:Journal of chemical ecology

    pub_type: 杂志文章

    doi:10.1007/s10886-010-9869-0

    authors: Madaras F,Gerber JP,Peddie F,Kokkinn MJ

    更新日期:2010-11-01 00:00:00