Abstract:
:The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-μl aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad Bradford dye reagent (Coomassie brilliant blue G-250) diluted 1:4 and containing 3 mg/ml soluble polyvinylpyrollidone. Absorbance at 595 nm is recorded after 15 min against an NaOH blank. Samples are calibrated against a ribulose 1,5-diphosphate carboxylase-oxygenase standard in NaOH. Procedures for plant preparation, extraction stability, the effects of phenol removal and quinone formation, and assay recovery are evaluated. Assay absorbance stability and techniques for increasing absorbance stability are reported. Changes in protein quality are briefly discussed.
journal_name
J Chem Ecoljournal_title
Journal of chemical ecologyauthors
Jones CG,Daniel Hare J,Compton SJdoi
10.1007/BF01015193subject
Has Abstractpub_date
1989-03-01 00:00:00pages
979-92issue
3eissn
0098-0331issn
1573-1561journal_volume
15pub_type
杂志文章abstract::Mammalian scent marking is often associated with territorial defense. However, males and females may demonstrate different activity patterns and play different roles. Female mammals nurture the young during lactation, while males purportedly perform other tasks more frequently, such as territorial maintenance and defe...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-006-9087-y
更新日期:2006-06-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00988076
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF02266971
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01025891
更新日期:1987-02-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00987655
更新日期:1984-01-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-012-0194-7
更新日期:2012-11-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-007-9387-x
更新日期:2007-12-01 00:00:00
abstract::A monoterpene alcohol was isolated from paraquat-induced lightwood in pines and identified as (+)-cis-3-pinen-2-ol [(+)-3]. More than a certain amount of (+)-3 induced a laboratory flight response by the male cerambycid beetle,Monochamus altematus Hope, but (+)-3 stimulated no response by the female at any dose level ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00994198
更新日期:1991-04-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/a:1019972312132
更新日期:2002-08-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00980075
更新日期:1992-07-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00990750
更新日期:1982-09-01 00:00:00
abstract::Quantities of attractive (exo-brevicomin and frontalin) and inhibitory (trans-verbenol, verbenone, and ipsdienol) pheromones were monitored in both sexes ofDendroctonus brevicomis during their colonization of a ponderosa pine. Verbenone was found in males in the greatest amounts at the time of landing, and it declined...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00987969
更新日期:1984-06-01 00:00:00
abstract::Entomophagous insects are often repelled by the secondary compounds of the plants eaten by their prey. These compounds, therefore, take on a defensive role for the phytophagous species that sequester them. Given that numerous entomophagous species are capable of learning, the effects on the foraging behavior of a repe...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/a:1021009618298
更新日期:2002-11-01 00:00:00
abstract::The parsnip webworm,Depressaria pastinacella (Lepidoptera: Oecophoridae), feeds on plants rich in furanocoumarins, phototoxic allomones. Final-instar larvae possess high levels of activities of antioxidant enzymes (Superoxide dismutase, catalase, glutathione reductase), which detoxify oxygen radicals generated from th...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01017468
更新日期:1990-08-01 00:00:00
abstract::Tetrapedia diversipes and other Apidae (Anthophoridae) may be deceived by floral similarities between Malpighiaceae and Orchidaceae of the Oncidiinae subtribe. The latter do not usually exudate floral oils. Thus, visitors may pollinate the flowers in a deceit/food/pollination syndrome. We studied the chemical composit...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-007-9309-y
更新日期:2007-07-01 00:00:00
abstract::In aquatic environments, chemical cues are believed to be associated with prey response to predation risk, yet few basic cue compositions are known despite the pronounced ecological and evolutionary significance of such cues. Previous work indicated that negatively-charged ions of m/z 501 are possibly a kairomone that...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-018-0925-5
更新日期:2018-02-01 00:00:00
abstract::Despite the recognition that phytohormonal signaling mediates induced responses to herbivory, we still have little understanding of how such signaling varies among closely related species and may generate herbivore-specific induced responses. We studied closely related milkweeds (Asclepias) to link: 1) plant damage by...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-014-0449-6
更新日期:2014-07-01 00:00:00
abstract::Atmospheric levels of carbon dioxide (CO2) have been increasing steadily over the last century. Plants grown under elevated CO2 conditions experience physiological changes, particularly in phytochemical content, that can influence their suitability as food for insects. Flavonoids are important plant defense compounds ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-009-9727-0
更新日期:2010-01-01 00:00:00
abstract::FourEscherichia coli strains carrying all the possible combinations of genes controlling sensitivity to near-UV (NUV;nur versusnur (+)) and far-UV (FUV;uvrA6 versusuvrA (+)) were inactivated with broad-spectrum NUV together with specific phototoxins. The inactivation kinetics of the four strains are consistent with th...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01020262
更新日期:1986-04-01 00:00:00
abstract::Several unsaturated aldehydes are produced from polyunsaturated fatty acids via the lipoxygenase pathway when soybean (Glycine max) plants are wounded mechanically or by pathogens. The effects of four of these aldehydes were examined on the growth of isolated fungal cultures ofColletotrichum truncatum, Rhizoctonia sol...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00979668
更新日期:1993-10-01 00:00:00
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journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF00980203
更新日期:1987-07-01 00:00:00
abstract::In field tests in Europe, traps baited with a blend of isoamyl alcohol, acetic acid, and isobutanol (compounds previously found attractive to a number of noctuids in North America) caught the following noctuid moths: Agrotis segetum Schiff., Agrotis crassa Hbn., Agrotis exclamationis L., Amathes (Xestia) c-nigrum L., ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-010-9789-z
更新日期:2010-06-01 00:00:00
abstract::The nitidulid beetle Lobiopa insularis is an important pest of strawberry crops in the United States and Brazil. Both larvae and adults feed on ripe strawberries, causing 20-70% loss in production during serious infestations. Aiming at the development of efficient, clean, and highly specific pest management systems, s...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-017-0851-y
更新日期:2017-06-01 00:00:00
abstract::In this study, we compared the effects of several preservation methods on the secondary phenolics of the mature leaves of purple willow (Salix purpurea L., salicaceae) with results obtained with fresh leaf analyses. Conventional freeze-drying, in which the leaves were first frozen with liquid nitrogen and then placed ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1023/a:1010358120482
更新日期:2001-04-01 00:00:00
abstract::Beetles share with other eukaryotes an innate immune system that mediates endogenous defense against pathogens. In addition, larvae of some taxa produce fluid exocrine secretions that contain antimicrobial compounds. In this paper, we provide evidence that larvae of the brassy willow leaf beetle Phratora vitellinae co...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-007-9416-9
更新日期:2008-02-01 00:00:00
abstract::Artemisinin, a sesquiterpene lactone produced in the leaves ofArtemisia annua, was evaluated for its phytotoxicity in mono- and dicotyledonous plants. Artemisinin inhibited seed germination, seedling growth, and root induction in all species tested. The concentration of artemisinin required for 50% inhibition ofLemna ...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/BF01020500
更新日期:1990-06-01 00:00:00
abstract::Tannins, a diverse group of water-soluble phenolics with high affinity to proteins, are widely distributed in various parts of plants, and have negative effects in herbivores after ingestion. Some mammalian species are thought to counteract tannins by secreting tannin-binding salivary proteins (TBSPs). Several types o...
journal_title:Journal of chemical ecology
pub_type: 杂志文章,评审
doi:10.1007/s10886-006-9077-0
更新日期:2006-06-01 00:00:00
abstract::Fleshy fruits contain a myriad of secondary metabolites that may fulfill various non-mutually exclusive ecological functions. Among them are defense against pathogens and herbivores, manipulation of frugivores' gut retention time, or controlling the germination process. In addition, it has been suggested that aroma co...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-016-0687-x
更新日期:2016-04-01 00:00:00
abstract::We investigated volatile infochemicals possibly involved in location of the generalist predatory mite Neoseiulus californicus to plants infested with spider mites in a Y-tube olfactometer. The predators significantly preferred volatiles from lima bean leaves infested with Tetranychus urticae to uninfested lima bean le...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-005-6075-6
更新日期:2005-09-01 00:00:00
abstract::Results of experiments conducted on ink recovered from the squid Sepioteuthis australis indicate that there is no epinephrine or protein naturally present in the ink as it would be ejected in vivo. Protein content was effectively zero when ink was syringed from the duct end of the ink sac of freshly killed animals. By...
journal_title:Journal of chemical ecology
pub_type: 杂志文章
doi:10.1007/s10886-010-9869-0
更新日期:2010-11-01 00:00:00